Beyond for modern lager brews: Exploring non- hybrids with heterotic maltotriose consumption and novel aroma profile.

Non-domesticated, wild yeasts have promising characteristics for beer diversification, particularly when used in the generation of interspecific hybrids. A major motivation for the current work was the question whether attractive novel interspecific hybrids can be created for the production of exotic lager beers without using the genomic resources of the ale yeast . Importantly, maltotriose utilization is an essential characteristic typically associated with domesticated ale/lager brewing strains.

Himalayan Genome Sequences Reveal Genetic Markers Explaining Heterotic Maltotriose Consumption by Saccharomyces pastorianus Hybrids.

strains are hybrids of and that have been domesticated for centuries in lager beer brewing environments. As sequences and structures of genomes are being resolved, molecular mechanisms and evolutionary origins of several industrially relevant phenotypes remain unknown. This study investigates how maltotriose metabolism, a key feature in brewing, may have arisen in early × hybrids.

Phenotype-Independent Isolation of Interspecies Hybrids by Dual-Dye Fluorescent Staining and Fluorescence-Activated Cell Sorting.

Interspecies hybrids of species are found in a variety of industrial environments and often outperform their parental strains in industrial fermentation processes. Interspecies hybridization is therefore increasingly considered as an approach for improvement and diversification of yeast strains for industrial application. However, current hybridization methods are limited by their reliance on pre-existing or introduced selectable phenotypes.

In vivo recombination of Saccharomyces eubayanus maltose-transporter genes yields a chimeric transporter that enables maltotriose fermentation.

Saccharomyces eubayanus is the non-S. cerevisiae parent of the lager-brewing hybrid S. pastorianus.

Selection of Pof Variants for the Construction of × Hybrids With Reduced 4-Vinyl Guaiacol Formation.

is an interspecies hybrid between and . The identification of the parental species of enabled the reconstruction of hybrids that could potentially combine a wide array of phenotypic traits. Lager yeasts are characterized by their inability to decarboxylate ferulic acid present in wort, a phenotype also known as Pof (phenolic off-flavor).

A potentially immunologically inert derivative of the reverse tetracycline-controlled transactivator.

The archetypical system for regulating heterologous gene expression in mammalian cells involves tetracycline-activated transactivators (rtTA). Binding of such transactivators to tet-operator-controlled promoters induces transcription. Immune responses directed against the transactivator proteins may limit the applicability of this system in immune-competent hosts.