Detection of genomic alterations in human endometrial cancer by two-dimensional gel electrophoresis.

Objective: The purpose of this study was to detect genomic alterations in human endometrial cancer by two-dimensional gel electrophoresis.

Study Design: With use of a newly developed two-dimensional gel electrophoresis assay, we scanned 19 high-risk DNA fragments for alterations in human endometrial hyperplasias and adenocarcinomas. This method includes cleaving of high-molecular-weight DNA, radioactive labeling, and separating DNA fragments by two-dimensional gel electrophoresis.