Atropisomeric Racemization Kinetics of MRTX1719 Using Chiral Solvating Agent-Assisted F NMR Spectroscopy.

With renewed interest in atropisomerism of drug molecules, efficient methods to experimentally determine torsion rotational energy barriers are needed. Here, we describe use of the chiral phosphoric acid solvating agent (+)-TiPSY to resolve the signals of atropisomers in F NMR and to use the data to study the kinetics of racemization and determine the rotational energy barrier of clinical compound . This method is complimentary to traditional chiral high-performance liquid chromatography (HPLC) and enhances the toolkit for chiral analysis techniques.

"Docking sites": nanometer-scale organization of a reactive, protein-resistant, graft copolymer-based interface for macromolecule immobilization.

The signal-to-noise ratio of a sensor system is determined by the affinity of its active component for the analyte on one hand and its inertness with respect to unrelated stimuli (noise) on the other hand. Nonspecific interactions between the environment and biosensor components (typically constructed from glass, silica, or transition metal oxides) result in nonspecific adsorption onto the latter and constitute a major source of noise. We have previously introduced a polymeric interface for preventing nonspecific adsorption while allowing for high-affinity, specific interactions.

Tuned graft copolymers as controlled coatings for DNA microarrays.

DNA microarrays have become a powerful tool for expression profiling and other genomics applications. A critical factor for their sensitivity is the interfacial coating between the chip substrate and the bound DNA. Such a coating has to embrace the divergent requirements of tightly binding the capture probe DNA during the spotting process and of minimizing the nonspecific binding of target DNA during the hybridization assay.

Reduction of friction at oxide interfaces upon polymer adsorption from aqueous solutions.

Reduction of the interfacial friction for the contact of a silicon oxide surface with sodium borosilicate in aqueous solutions has been accomplished through the adsorption of poly(L-lysine)-graft-poly(ethylene glycol) on one or both surfaces. Spontaneous polymer adsorption has been achieved via the electrostatic attraction of the cationic polylysine polymer backbone and a net negative surface charge, present for a specific range of solution pH values. Interfacial friction has been measured in aqueous solution, in the absence of wear, and on a microscopic scale with atomic force microscopy.