Proficient bioconversion of rice straw biomass to bioethanol using a novel combinatorial pretreatment approach based on deep eutectic solvent, microwave irradiation and laccase.

Current study is the first report of the combined application of chemical (deep eutectic solvent), physical (microwave irradiation) and biological (laccase) pretreatment strategies for enhancing the enzymatic digestibility of rice straw biomass. Pretreated rice straw biomass was saccharified by cellulase/xylanase from Aspergillus japonicus DSB2 to get a sugar yield of 252.36 mg/g biomass.

An eco-friendly novel approach for bioconversion of Saccharum spontaneum biomass to biofuel-ethanol under consolidated bioprocess.

Quest for renewable/eco-friendly energy sources has received immense focus in recent years. Current study involved consolidated bioprocessing of Saccharum spontaneum biomass (SSB) for biofuel-ethanol generation in a 'one pot consolidated bioprocess' (OPCB). SSB was pretreated with protic ionic liquid, triethylamine-bisulfate ([TEA][HSO]), saccharified in-situ with cellulase/xylanase enzymes, and the released sugars were fermented to ethanol.

Chemo-enzymatic approaches for consolidated bioconversion of Saccharum spontaneum biomass to ethanol-biofuel.

A novel strategy involving sodium dodecylsulfate (SDS) (SDS assisted tris (2-hydroxyethyl) methyl- ammonium methyl sulphate ([TMA][MeSO], ionic liquid) pretreatment of Saccharum spontaneum biomass (SSB) following its enzymatic saccharification, and conversion into ethanol-biofuel in a consolidated bioprocess (CBP) was developed. Ionic liquid stable enzyme preparation developed from Bacillus subtilis G was used for saccharification. Optimized pretreatment and saccharification variables enhanced the sugar yield (2.