Physicochemical Characterization and Antioxidant and Hypolipidaemic Activities of a Polysaccharide From the Fruit of (Lem.) A. C. Smith.

fruit, a novel fruit resource, has attracted wide interest, but the physicochemical characteristics and biological activities of its polysaccharides remain unclear. This study investigated the physicochemical properties of a polysaccharide extracted from fruit polysaccharide (KCFP) and evaluated its antioxidant and hypolipidaemic activities and . KCFP is an amorphous, thermally stable pectin heteropolysaccharide with an average molecular weight of 204.

Ultrasensitive fluorescent detection of nucleic acids based on label-free enzymatic-assisted cascade signal amplification.

A simple and homogenous label-free fluorescent method based on target-triggered and enzymatic-assisted isothermal cascade signal amplification was established for nucleic acid detection. This amplification method consists of two circuits that depend on polymerase and nicking endonuclease. In the presence of a target, a hairpin probe was opened to initiate the polymerization and nicking reaction, producing target analogues and G-rich sequences, and releasing the original target DNA.

A sensitive strategy for the fluorescence detection of DNA methyltransferase activity based on the graphene oxide platform and T7 exonuclease-assisted cyclic signal amplification.

In this work, a simple fluorescence strategy based on the graphene oxide (GO) platform and T7 exonuclease (T7 Exo)-assisted cyclic signal amplification is developed for the fast and sensitive detection of DNA methyltransferase (MTase) activity and inhibition. In the sensing design, Dam MTase was used as a model analyte. In the presence of Dam MTase, a hairpin probe (HP) was methylated, and then specially recognized and cleaved by Dpn I endonuclease, releasing a ssDNA fragment.