Assessment of a liquid lens enabled in vivo optical coherence microscope.

The optical aberrations induced by imaging through skin can be predicted using formulas for Seidel aberrations of a plane-parallel plate. Knowledge of these aberrations helps to guide the choice of numerical aperture (NA) of the optics we can use in an implementation of Gabor domain optical coherence microscopy (GD-OCM), where the focus is the only aberration adjustment made through depth. On this basis, a custom-designed, liquid-lens enabled dynamic focusing optical coherence microscope operating at 0.

Gabor-based fusion technique for Optical Coherence Microscopy.

We recently reported on an Optical Coherence Microscopy technique, whose innovation intrinsically builds on a recently reported - 2 microm invariant lateral resolution by design throughout a 2 mm cubic full-field of view - liquid-lens-based dynamic focusing optical probe [Murali et al., Optics Letters 34, 145-147, 2009]. We shall report in this paper on the image acquisition enabled by this optical probe when combined with an automatic data fusion method developed and described here to produce an in-focus high resolution image throughout the imaging depth of the sample.

Three-dimensional adaptive microscopy using embedded liquid lens.

We report on the compact optical design of a high-resolution 3D scanning microscope with adaptive optics capability for refocusing with no moving parts designed for clinical research. The optical aberrations arising from refocusing are compensated for as part of the multiconfiguration optical design process. The lateral scanning is provided by a scanning mirror, and the depth scan is provided by an adaptive liquid lens embedded within the microscope as an integrated component of a custom optical design.

Optical design of a dynamic focus catheter for high-resolution endoscopic optical coherence tomography.

The optical system design of a dynamic focus endoscopic probe for optical coherence tomography is reported. The dynamic focus capability is based on a liquid lens technology that provides variable focus by changing its curvatures in response to an electric field variation. The effects of a cylindrical exit window present, in practice, for a catheter were accounted for.

Collaborative engineering: 3-D optical imaging and gas exchange simulation of in-vitro alveolar constructs.

This paper reports on the computational simulation and modeling of an in vitro alveolar construct system along the optical coherence microscopy (OCM) methods for visualizing engineered tissue. The optical imaging methods will be compared to immunohistochemical light microscopy samples of engineered alveolar constructs. Results show depth images of the alveolar tissue construct for a bilayer construct, as well as predictions of the gas exchange process in a simple model of a bio-reactor hosting the construct.