Role of Non-Binding T63 Alteration in IL-18 Binding.

Engineered interleukin-18 (IL-18) has attracted interest as a cytokine-based treatment. However, knowledge-based mutagenesis of IL-18 has been reported for only a few regions of the protein structures, including binding sites I and II. When coupled with the binding region mutant (E6K), the non-binding residue of IL-18, Thr63 (T63), has been shown to increase the flexibility of the binding loop.

Correction: Advanced biomaterial agent from chitosan/poloxamer 407-based thermosensitive hydrogen containing biosynthesized silver nanoparticles using Eucalyptus camaldulensis leaf extract.

[This corrects the article DOI: 10.1371/journal.pone.

Gall Ethanolic Extract Accelerates Wound Healing through Attenuating Inflammation and Oxidative Injuries in Skin Fibroblasts.

Olivier (Fagaceae) nutgall, a traditional Asian medicine, is renowned for its efficacy in treating wounds and skin disorders. Although the gall extract has shown promising results in accelerating wound healing in diabetic animal models, its mechanisms, particularly the effects on redox balance, remain poorly understood. This study aims to investigate the effects and mechanisms of gall ethanolic extract (QIG) on wound healing in fibroblasts, with a specific emphasis on its modulation of oxidative stress.

Bioactive substance contents and therapeutic potential for skin inflammation of an herbal gel containing and .

Context: Craib. and L., of the Fabaceae, have been used as active components in Thai herbal formulas for the treatment of fever and skin diseases.

Antibacterial and Anti-Biofilm Efficacy of Endolysin LysAB1245 against a Panel of Important Pathogens.

Infections caused by antibiotic-resistant bacteria pose a significant global challenge. This study explores the antibacterial effects of a bacteriophage-derived endolysin, LysAB1245, against important pathogens, including , , , and We determined the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for all tested isolates. A time-kill study was conducted to evaluate the reduction in bacterial survival following treatment with LysAB1245.

A Novel Antibiotic, Rhodomyrtone: Pharmacokinetic Studies in a Murine Model and Optimization and Validation of High-Performance Liquid Chromatographic Method for Plasma Analysis.

Rhodomyrtone has indisputable and undeniable potential as a new antibiotic for antibiotic-resistant Gram-positive bacteria. Therefore, the main objective of this study was to determine the pharmacokinetics profiles of orally administered rhodomyrtone in rats. A reverse-phase HPLC-UV method was developed, optimized and validated for the analysis of rhodomyrtone concentrations in rat plasma.

Characterization of a novel bacteriophage endolysin (LysAB1245) with extended lytic activity against distinct capsular types associated with Acinetobacter baumannii resistance.

Capsular polysaccharides are considered as major virulence factors associated with the ability of multidrug-resistant (MDR) Acinetobacter baumannii to cause severe infections. In this study, LysAB1245, a novel bacteriophage-encoded endolysin consisting of a lysozyme-like domain from phage T1245 was successfully expressed, purified, and evaluated for its antibacterial activity against distinct capsular types associated with A. baumannii resistance.

Transferosomes stabilized hydrogel incorporated rhodomyrtone-rich extract from Rhodomyrtus tomentosa leaf fortified with phosphatidylcholine for the management of skin and soft-tissue infections.

Rhodomyrtus tomentosa leaf (RT)-incorporated transferosomes were developed with lecithin and cholesterol blends with edge activators at different ratios. RT-transferosomes were characterized and employed in transferosomal gel formulations for the management of skin and soft-tissue infections. The optimized formulation entrapped up to 81.

Effectiveness of plant-based hand sanitizer incorporating Quercus infectoria gall extract.

Aims: Quercus infectoria (Qi), a traditional herbal plant with a broad spectrum of activities on multidrug-resistant bacteria, has been developed for hand sanitizer applications.

Methods And Results: Antimicrobial activity was evaluated using agar-well diffusion and broth microdilution method. Bactericidal activity was determined following the European Standard 1276 antibacterial suspension test.

Synergistic effects of polymyxin and vancomycin combinations on carbapenem- and polymyxin-resistant and their molecular characteristics.

This study provides insights into the mechanisms of polymyxin resistance in clinical isolates and demonstrates potential strategies of polymyxin and vancomycin combinations for combating this resistance. We also identified possible mechanisms that might be associated with the treatment of these combinations against carbapenem- and polymyxin-resistant clinical isolates. The findings have significant implications for the development of alternative therapies and the effective management of infections caused by these pathogens.

Advanced biomaterial agent from chitosan/poloxamer 407-based thermosensitive hydrogen containing biosynthesized silver nanoparticles using Eucalyptus camaldulensis leaf extract.

Context: The emergence of multidrug-resistant (MDR) pathogens poses a significant challenge for global public health systems, increasing hospital morbidity and mortality and prolonged hospitalization.

Objective: We evaluated the antimicrobial activity of a thermosensitive hydrogel containing bio-synthesized silver nanoparticles (bio-AgNPs) based on chitosan/poloxamer 407 using a leaf extract of Eucalyptus calmadulensis.

Results: The thermosensitive hydrogel was prepared by a cold method after mixing the ingredients and left at 4°C overnight to ensure the complete solubilization of poloxamer 407.

Genomic and Transcriptional Profiling Analysis and Insights into Rhodomyrtone Yield in (Aiton) Hassk.

is a source of a novel antibiotic, rhodomyrtone. Because of the increasing industrial demand for this compound, germplasm with a high rhodomyrtone content is the key to sustainable future cultivation. In this study, rhodomyrtone genotypes were verified using the plastid genomic region marker and nuclear ribosomal internal transcribed spacer ITS.

Camaldulensals A-C, the First Meroterpenoids Possessing Two Spatially Separated Formyl Phloroglucinols Conjugated to a Terpene Core from the Leaves of Dehnh.

Three new bis-formyl phloroglucinol-meroterpenoids (-), three new euglobal type formyl phloroglucinol-meroterpenoids (-), and one new dimeric formyl phloroglucinol () were isolated from the leaves of . Camaldulensal A () is the first bis-isovaleryl-formyl-phloroglucinol-sesquiterpenoid. It features a novel 6/6/10/3/6/6 fused ring system and contains six stereogenic centers.

In vitro and in vivo comparative analysis of antibacterial activity of green-synthesized silver nanoparticles.

This study aims to compare antibacterial effects of green-synthesized silver nanoparticles (AgNPs) with silver nitrate (AgNO ). AgNPs were successfully synthesized using Eucalyptus camaldulensis leaf extract as a reducing and stabilizing agent. Minimum inhibitory concentrations (MIC) of AgNPs and AgNO against Staphylococcus aureus and Pseudomonas aeruginosa ranged between 4.

Control of biomaterial-associated infections through biofabrication of gold nanoparticles using Musa sapientum extract.

Biofilm-associated infections are a critical element in infectious diseases and play an important role in antibiotic resistance. Biosynthesized gold nanoparticles (AuNPs) using ethanolic extract of Musa sapientum unripe fruit were performed. The nanoparticles demonstrated an absorption peak at 554 nm with particle sizes ranging from 5.

Complete Genome of Rose Myrtle, , and Its Population Genetics in Thai Peninsula.

Several parts of rose myrtle, , exhibited profound antibacterial and anti-inflammatory activities, suggesting its potential in healthcare and cosmetics applications. During the past few years, the demand for biologically active compounds in the industrial sectors increased. Therefore, gathering comprehensive information on all aspects of this plant species is essential.

Rifampicin Enhanced Carbapenem Activity with Improved Antibacterial Effects and Eradicates Established Biofilms.

Biofilm-mediated infections are critical to public health and a leading cause of resistance among pathogens, amounting to a prolonged hospital stay and increased mortality rate in the intensive care unit. In this study, the antibacterial and antibiofilm activities of rifampicin or carbapenem monotherapies were compared with rifampicin and carbapenem combination therapies against rifampicin-resistant and carbapenem-resistant isolates. Among 29 CRAB isolates, 24/29 (83%) were resistant to rifampicin, with MIC values between 2-256 µg/mL.

Potential applications of leaf extract as natural anti-staphylococcal additive in food systems: Efficacy and safety evaluation.

This work aimed to explore the potential use of ethanol leaf extract (RTEL) as an alternative food preservative agent for controlling the growth of . Antibacterial activities against food-isolated were performed using disc diffusion and broth microdilution assays, followed by evaluating subacute oral toxicity of the extract. Salad dressing was used as a food model to study bactericidal properties and consumer acceptability.

Interaction of lytic phage T1245 with antibiotics for enhancement of antibacterial and anti-biofilm efficacy against multidrug-resistant .

Biofilms associated with multidrug-resistant (MDR) on medical devices remain a big clinical problem. Antibiotic susceptibility tests were performed with eight commonly employed antibiotics against clinical isolates. The effects of antibiotics in combination with well-characterized lytic phage T1245 were studied to assess their antibacterial and anti-biofilm efficacy.

Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital.

COVID-19 patients have often required prolonged endotracheal intubation, increasing the risk of developing ventilator-associated pneumonia (VAP). A preventive strategy is proposed based on an endotracheal tube (ETT) modified by the in situ deposition of eucalyptus-mediated synthesized silver nanoparticles (AgNPs). The surfaces of the modified ETT were embedded with AgNPs of approximately 28 nm and presented a nanoscale roughness.

Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation.

Catheter-associated urinary tract infections (CAUTIs) are significant complications among catheterized patients, resulting in increased morbidity, mortality rates, and healthcare costs. Foley urinary catheters coated with synthesized silver nanoparticles (AgNPs) using leaf extract were developed using a green chemistry principle. In situ-deposited AgNPs with particle size ranging between 20 and 120 nm on the catheter surface were illustrated by scanning electron microscopy.

A new alkaloid and a new benzaldehyde derivative from the twig of (Roxb.) DC.

A new alkaloid, 2-acetyl-4-methoxyfuro[2,3-]quinoline (), and a new benzaldehyde derivative, (2')-4-(2'-hydroxy-3'-methyl-3'-butenoxy)benzaldehyde (), were isolated from the twig of (Roxb.) DC. along with twenty-six known compounds ().

New acylphloroglucinols from a crude acetone extract of Dehnh. leaf.

Acylphloroglucinols are well-known secondary metabolites which exhibit a variety of structures and bioactivities. The investigation of a crude acetone extract of leaves led to the isolation of two new acylphloroglucinols, eucalypcamals O and P ( and ) together with seven phloroglucinols (-), and a benzene derivative (). Their chemical structures were elucidated by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy and mass spectroscopy.

Surface cysteine to serine substitutions in IL-18 reduce aggregation and enhance activity.

Background: Interleukin-18 (IL-18) is prone to form multimers resulting in inactive aggregates, making this cytokine unstable for clinical use. Therefore, mutations have been introduced into recombinant IL-18 to overcome this issue.

Methods: To prevent the formation of disulfide bonds between the IL-18 molecules, multiple mutations targeting surface cysteines (C38, C68, C76, and C127) were introduced into our previously modified human IL-18 double mutant E6K+T63A (IL-18 DM) by direct gene synthesis.

Successful treatment of refractory erythrodermic psoriasis with traditional Thai herbal medicine.

Background: Thai herbal formulations have been used traditionally in Thailand for the treatment of psoriasis. However, there is still a lack of scientific data supporting the effects of Thai herbal formulations in psoriasis treatment.

Objectives: This study aimed to demonstrate the therapeutic effects of Thai herbal formulations for the treatment of erythrodermic psoriasis.