Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering.

In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content. P(HB-50HV) was biosynthesized from bacteria H16 and the in vitro proliferation of dental cells for tissue engineering application was evaluated. Comparisons were made with scaffolds prepared by poly(hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-12%hydroxyvalerate) (P(HB-12HV)), and polycaprolactone (PCL).

Comparison of methods for wavelength combination selection from multi-wavelength fluorescence spectra for on-line monitoring of yeast cultivations.

The on-line monitoring with two-dimensional (2D) fluorescence spectroscopy of Saccharomyces cerevisiae batch cultivations was applied to monitor glucose, ethanol, and biomass concentrations. The measurement of one spectrum by the 2D fluorescence spectrometer has 120 fluorescence intensity values of excitation and emission wavelength combinations (WLCs); scattered light is not considered here. To identify which WLCs of the multi-wavelength fluorescence spectra carry important and relevant information regarding the analyte concentrations, three different methods were compared: a method based on loadings, variable importance in projection, and ant colony optimization.