Whole-Genome Methylation Sequencing Analysis and Functional Verification of LIM-Homeobox Family Genes in Cervical Cancer.

Background: Gene methylation in cells is an important factor in tumorigenesis, and radiotherapy can change DNA methylation in cells. In this study, complete genome methylation sequencing (BS-Seq) technology was used to analyze the genome-wide methylation of patients with cervical cancer before and after radiotherapy.

Methods: Three pairs of cervical squamous cell carcinoma samples were collected from patients before and after radiotherapy in July 2020.

Tenascin-C promotes bone regeneration via inflammatory macrophages.

During the early stage of tissue injury, macrophages play important roles in the activation of stem cells for further regeneration. However, the regulation of macrophages during bone regeneration remains unclear. Here, the extracellular matrix (ECM) tenascin-C (TNC) is found to express in the periosteum and recruit inflammatory macrophages.

Structural Similarity in Natural Products Leading to Sample Misidentification: A Case Study of the Bisbenzylisoquinoline Alkaloids Oxyacanthine and Berbamine.

The similar structures of natural compounds and the absence of NMR data for commercial products raise the risk of misidentification. This work reports a case in which purchased samples labeled as "berbamine" from 14 suppliers are oxyacanthine (). The NMR data of all purchased samples were consistent.

Synthesis of three-dimensional covalent organic frameworks through a symmetry reduction strategy.

Three-dimensional (3D) covalent organic frameworks (COFs) hold significant promise for a variety of applications. However, conventional design approaches using regular building blocks limit the structural diversity of 3D COFs. Here we design and synthesize two 3D COFs, designated as JUC-644 and JUC-645, through a methodology that relies on using eight-connected building blocks with reduced symmetry.

Mechanisms of thermal, acid, desiccation and osmotic tolerance of spp.

spp. exhibit remarkable resilience to extreme environmental stresses, including thermal, acidic, desiccation, and osmotic conditions, posing significant challenges to food safety. Their thermotolerance relies on heat shock proteins (HSPs), thermotolerance genomic islands, enhanced DNA repair mechanisms, and metabolic adjustments, ensuring survival under high-temperature conditions.

Supervised multi-frame dual-channel denoising enables long-term single-molecule FRET under extremely low photon budget.

Camera-based single-molecule techniques have emerged as crucial tools in revolutionizing the understanding of biochemical and cellular processes due to their ability to capture dynamic processes with high precision, high-throughput capabilities, and methodological maturity. However, the stringent requirement in photon number per frame and the limited number of photons emitted by each fluorophore before photobleaching pose a challenge to achieving both high temporal resolution and long observation times. In this work, we introduce MUFFLE, a supervised deep-learning denoising method that enables single-molecule FRET with up to 10-fold reduction in photon requirement per frame.

The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella.

Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood. In this study, 78 stable salinomycin-resistant strains were generated through experimental evolution approaches.

Intermittent catheter clamping combined with active urination training (ICCAUT) to reduce the risk of urinary dysfunction in patients after proctectomy: a single-center cohort study.

Background: Intermittent urethral catheter clamping is widely used to reduce secondary catheterization in patients after proctectomy; however, its effectiveness is unclear.

Materials And Methods: This study investigated the effects of intermittent catheter clamping combined with active urination training (ICCAUT) on postoperative urinary dysfunction in patients after proctectomy. This retrospective cohort study analyzed data on patients who underwent laparoscopic/robotic-assisted proctectomy at a single medical center in China between July 2023 and January 2024.

1.25(OH)2D3 decreases PCNA and mTOR expression and alleviates renal injury in Thy-1 nephritis rat model.

Objective: This study investigated the role and mechanisms of 1.25(OH)2D3 in proliferative glomerulonephritis and its effect on the regulation of mesangial cells.

Methods: Sixty male SD rats were randomly divided into four groups: control (CG), nephritis (NG), nephritis + 1.

The diagnosis and treatment progress of infectious endophthalmitis.

Endophthalmitis is a blinding disease that may lead to permanent vision loss. The diagnosis of endophthalmitis relies on clinical findings. It is crucial to identify causative microorganisms in time for subsequent treatment and saving vision.

Reproductive Tract Mucus May Influence the Sex of Offspring in Cattle: Study in Cows That Have Repeatedly Calved Single-Sex Offspring.

This study aimed to investigate the selective effect of the reproductive tract mucus in cows that have consistently produced offspring of a single sex on X/Y spermatozoa. We collected mucus from the reproductive tract of cows that had given calvings to offspring of the same sex, or alternated between sexes, for more than five consecutive calvings. We evaluated the pH of reproductive tract mucus.

Niche-specific evolution and gene exchange of Salmonella in retail pork and chicken.

Salmonella exhibits extensive genetic diversity, facilitated by horizontal gene transfer occurring within and between species, playing a pivotal role in this diversification. Nevertheless, most studies focus on clinical and farm animal isolates, and research on the pangenome dynamics of Salmonella isolates from retail stage of the animal food supply chain is limited. Here, we investigated the genomes of 950 Salmonella isolates recovered from retail chicken and pork meats in seven provinces and one municipality of China in 2018.

Event-Enhanced Snapshot Compressive Videography at 10K FPS.

Video snapshot compressive imaging (SCI) encodes the target dynamic scene compactly into a snapshot and reconstructs its high-speed frame sequence afterward, greatly reducing the required data footprint and transmission bandwidth as well as enabling high-speed imaging with a low frame rate intensity camera. In implementation, high-speed dynamics are encoded via temporally varying patterns, and only frames at corresponding temporal intervals can be reconstructed, while the dynamics occurring between consecutive frames are lost. To unlock the potential of conventional snapshot compressive videography, we propose a novel hybrid "intensity + event" imaging scheme by incorporating an event camera into a video SCI setup.

Discovery of peptidomimetic spiropyrrolidine derivatives as novel 3CL inhibitors against SARS-CoV -2.

Given the high pathogenicity and rapid mutation rates of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is imperative to sustain efforts in drug research and development. Herein, we present the design, synthesis, and evaluation of peptidomimetic spiropyrrolidine derivatives as potent 3CL inhibitors against SARS-CoV-2. Among the synthesized derivatives, several compounds exhibited high potency in inhibiting 3CL, with IC values ranging from 21 nM to 53 nM.

Prognostic role of prostate specific antigen kinetics in primary high volume metastatic hormonal sensitive prostate cancer treated with novel hormonal therapy agents.

The prognostic value of prostate-specific antigen (PSA) kinetics in primary high-volume metastatic hormone-sensitive prostate cancer (mHSPC) patients treated with novel hormonal therapy agents is still unclear. Here, we retrospectively reviewed the data of 102 patients with primary high-volume mHSPC who received novel hormonal therapy agents. The median follow-up was 32.

Selection, inheritance, life-history traits, and flight capacity of Grapholita molesta (Lepidoptera: Tortricidae) resistant to lambda-cyhalothrin.

Grapholita molesta (Busck) (Lepidoptera: Tortricidae) is a globally significant fruit pest traditionally managed using chemical insecticides such as lambda-cyhalothrin. However, repeated applications have led to the development of resistance in field populations. The mechanisms and fitness costs associated with lambda-cyhalothrin resistance in G.

The role and mechanism of TgCWIN2-mediated changes of photo-assimilates in modulating early development of Torreya grandis seeds.

Early seed development is vital for plant reproduction, but the processes behind this in gymnosperms like Torreya grandis, which has a low rate of normal early-developed seeds, are not well understood. To fill this knowledge gap, we embarked on a comprehensive investigation encompassing the morphology and phenology of seed development in T. grandis.

High-Performance Polyimide Covalent Organic Frameworks for Lithium-Ion Batteries: Exceptional Stability and Capacity Retention at High Current Densities.

Organic polymers are considered promising candidates for next-generation green electrode materials in lithium-ion batteries (LIBs). However, achieving long cycling stability and capacity retention at high current densities remains a significant challenge due to weak structural stability and low conductivity. In this study, we report the synthesis of two novel polyimide covalent organic frameworks (PI-COFs), COF-JLU85 and COF-JLU86, by combining truxenone-based triamine and linear acid anhydride through polymerization.