A microplate spectrophotometric method for analysis of indole-3-carbinol in dietary supplements using -dimethylaminocinnamaldehyde (DMACA) as a chromogenic reagent.

This work presents the development of a microplate spectrophotometric method for determination of indole-3-carbinol in dietary supplements. The colorimetric procedure is based on the reaction of indole-3-carbinol with the -dimethylaminocinnamaldehyde (DMACA) reagent under acidic conditions. The absorbance of the colored product measured at 675 nm was used to determine the target analyte.