Stem Cell-Laden Engineered Patch: Advances and Applications in Tissue Regeneration.

Stem cell-based therapies are emerging as significant approaches in tissue engineering and regenerative medicine, applicable to both fundamental scientific research and clinical practice. Despite remarkable results in clinical studies, challenges such as poor standardization of graft tissues, limited sources, and reduced functionality have hindered the effectiveness of these therapies. In this review, we summarize the engineering approaches involved in fabricating stem cell assisted patches and the substantial strategies for designing stem cell-laden engineered patches (SCP) to complement the existing stem cell-based therapies.

sp. nov. and sp. nov., isolated from the mudflat of colonies.

Two novel strains were isolated from the mudflat of colonies in Incheon, Republic of Korea. Designated as DGU5 and DGU6, these strains were Gram-stain-negative, facultatively anaerobic and rod-shaped and had yellowish colonies. Both strains were determined to belong to the genus through phylogenetic analysis of their 16S rRNA sequences and draft genomes.

sp. nov. and sp. nov., novel carotenoid-producing bacterial species isolated from farm soil.

Two yellow-pigmented novel strains, designated HF-S3 and HF-S4, were isolated from farm soil in Paju, Republic of Korea. Cells of the two strains are characteristically Gram-stain-negative, facultatively anaerobic, catalase- and oxidase-positive, non-motile and rod-shaped. Strain HF-S3 grew at 10-37 °C, while HF-S4 grew at 15-35 °C.

sp. nov. and sp. nov., isolated from the roots of Makino and W. Becker with plant growth-promoting potential.

Two novel Gram-positive strains, chi10 and PFR10, were isolated from the roots of Makino and W. Becker, respectively. The strains are facultatively aerobic, rod-shaped, motile via peritrichous flagella and endospore-forming.

A novel exopolysaccharide-producing bacterium, sp. nov. isolated from , enhances the growth of subsp. under drought and salinity stress.

Biological and abiotic stresses in plant growth are associated with reduced crop yields. Therefore, improving plant stress resistance can be a crucial strategy to improve crop production. To overcome these problems, plant growth-promoting bacteria are emphasized as one of the alternative tools for sustainable agriculture.

Gelatin Nanoparticles can Improve Pesticide Delivery Performance to Plants.

Nanomaterials associated with plant growth and crop cultivation revolutionize traditional concepts of agriculture. However, the poor reiterability of these materials in agricultural applications necessitates the development of environmentally-friendly approaches. To address this, biocompatible gelatin nanoparticles (GNPs) as nanofertilizers with a small size (≈150 nm) and a positively charged surface (≈30 mV) that serve as a versatile tool in agricultural practices is designed.

sp. nov. and sp. nov., isolated from artificial pond water and sp. nov., isolated from a root.

Two novel strains, designated APW6 and APW11, were isolated from artificial pond water, and one novel strain, designated PFR6, was isolated from a root. These strains were found to be Gram-negative, rod-shaped, motile by means of flagella, and oxidase-positive. Growth conditions of the type strains were as follows: APW6, 15-43 °C (optimum, 28 °C), pH 6.

Roseateles caseinilyticus sp. nov. and Roseateles cellulosilyticus sp. nov., isolated from rice paddy field soil.

Two novel Gram-stain-negative strains designated P7 and P8, were isolated from the soil of a paddy field in Goyang, Republic of Korea, and identified as new species within the genus Roseateles through a polyphasic taxonomic approach. These aerobic, rod-shaped, non-sporulating strains demonstrated optimal growth at 30 °C, pH 7, and in the absence of NaCl (0% w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated close relationships with Roseateles saccharophilus DSM654 (98.

sp. nov. and sp. nov., isolated from a pond.

Two Gram-negative bacteria, designated as strains LF1 and HM2-2, were isolated from an artificial pond in a honey farm at Hoengseong-gun, Gangwon-do, Republic of Korea. The 16S rRNA sequence analysis results revealed that strain LF1 belonged to the genus and had the highest sequence similarity to GH41-7 (99.0 %), CJ29 (98.

Pedobacter rhodius sp. nov. and Pedobacter punctiformis sp. nov., isolated from soil.

Two Gram-staining negative, catalase- and oxidase-positive, pinkish-colored and rod-shaped strains, designated SJ11 and HCMS5-2, were isolated from soil in South Korea. The growth of strain SJ11 was observed from 15℃ to 35℃ (optimum, 30℃), from pH 6.0 to 11.

Draft Genome Sequence Analyses of Two Novel sp. nov. and sp. nov. Isolated from the Root of Makino.

Gram-negative, rod-shaped, and aerobic bacteria designated chi1 and chi5 were isolated from the root of Makino. Phylogenetics utilizing 16S rRNA and whole-genome sequences of the two novel strains chi1 and chi5 confirmed that they were related to the genera and , respectively. For the novel strains chi1 and chi5, the digital DNA-DNA hybridization values (19-20% and 22.

Micro/nanoengineered agricultural by-products for biomedical and environmental applications.

Agriculturally derived by-products generated during the growth cycles of living organisms as secondary products have attracted increasing interest due to their wide range of biomedical and environmental applications. These by-products are considered promising candidates because of their unique characteristics including chemical stability, profound biocompatibility and offering a green approach by producing the least impact on the environment. Recently, micro/nanoengineering based techniques play a significant role in upgrading their utility, by controlling their structural integrity and promoting their functions at a micro and nano scale.

Diagnosis of posterior staphyloma using the radius of steepest curvature among retinal pigment epithelium segmentation line measured by optic coherent tomography.

Purpose: To investigate a novel marker to diagnose posterior staphylomas by measuring the radius of the steepest curvature on the retinal pigment epithelium (RPE) segmentation line using optical coherence tomography (OCT).

Study Design: Retrospective Cross-sectional Study.

Methods: The authors developed a prototype software to measure the radius of curvature on the RPE segmentation line of OCT.

Graphene Hybrid Tough Hydrogels with Nanostructures for Tissue Regeneration.

Over the past few decades, hydrogels have attracted considerable attention as promising biomedical materials. However, conventional hydrogels require improved mechanical properties, such as brittleness, which significantly limits their widespread use. Recently, hydrogels with remarkably improved toughness have been developed; however, their low biocompatibility must be addressed.

sp. nov. and sp. nov., isolated from wetland soils.

Two novel strains were isolated from wetland soils in Goyang, Republic of Korea. The two Gram-stain-positive, facultatively anaerobic, rod-shaped bacterial-type strains were designated MW4 and MW9. Phylogenomic analysis based on whole-genome sequences suggested that both strains belonged to the genus .

Piezoelectrically and Topographically Engineered Scaffolds for Accelerating Bone Regeneration.

Bone regeneration remains a critical concern across diverse medical disciplines, because it is a complex process that requires a combinatorial approach involving the integration of mechanical, electrical, and biological stimuli to emulate the native cellular microenvironment. In this context, piezoelectric scaffolds have attracted considerable interest owing to their remarkable ability to generate electric fields in response to dynamic forces. Nonetheless, the application of such scaffolds in bone tissue engineering has been limited by the lack of a scaffold that can simultaneously provide both the intricate electromechanical environment and the biocompatibility of the native bone tissue.

Engineering Considerations on Large-Scale Cultured Meat Production.

In recent decades, cultured meat has received considerable interest as a sustainable alternative to traditional meat products, showing promise for addressing the inherent problems associated with conventional meat production. However, current limitations on the scalability of production and extremely high production costs have prevented their widespread adoption. Therefore, it is important to develop novel engineering strategies to overcome the current limitations in large-scale cultured meat production.

Graphene-encapsulated yeast cells in harsh conditions.

Yeast, as a versatile microorganism, holds significant importance in various industries and research fields due to its remarkable characteristics. In the pursuit of biotechnological applications, cell-surface engineering including encapsulation has been proposed as a new strategy to interface with individual living yeast cells. While previous researches of yeast encapsulation with materials have shown promise, it often involves complex processes and lacks confirmation of condition-dependent yeast viability under harsh conditions.

sp. nov., isolated from the fermented fruit of a medicinal plant.

A facultative anaerobic and Gram-negative strain, designated RP14, was isolated from the fruit of fermented for 60 days at 25°C. Strain RP14 showed 98.0 % 16S rRNA similarity to IFO 15243, but in the phylogenetic tree, NIBRBAC000500504 was its closest neighbour.

sp. nov. and sp. nov., two novel actinomycetes isolated from .

Two novel Gram-positive bacteria designated as strains STR2 and STR3 were isolated from the rhizosphere of a sample collected from Goyang-si, Republic of Korea. Strains STR2 and STR3 were aerobic, rod shaped, non-sporulated, catalase negative, oxidase negative and non-motile bacteria. They grew at 15-37 °C (optimum, 25-30 °C), at pH 6.

sp. nov., sp. nov. and sp. nov., isolated from freshwater at Jucheon River, and emended description of comb. nov.

Three Gram-stain-negative, facultatively anaerobic, rod-shaped, catalase-positive, oxidase-negative bacterial strains were designated as hw1, hw8 and hw3. Strains hw1, hw8 and hw3 grew at 15-28 °C (optimum, 25 °C), 15-35 °C (optimum, 30 °C) and 4-28 °C (optimum, 20 °C), respectively, and at pH 7.0-12.

sp. nov. and sp. nov., isolated from a tidal mudflat.

Strains chi3 and sf7 were collected from a tidal mudflat around Dongmak beach in Ganghwa, Republic of Korea. Both strains were Gram-stain-negative, aerobic or facultatively anaerobic, and rod-shaped. Results of phylogenetic tree analysis based on 16S rRNA and whole-genome sequences suggested that strains chi3 and sf7 belong to the genera and , respectively.

Planobacterium oryzisoli sp. nov., a novel bacterium isolated from roots of rice plant.

A Gram-negative, aerobic, short rod-shaped, non-motile, non-spore forming bacterium, designated strain GCR5, was isolated from soil of paddy field. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GCR5 belongs to the genus Planobacterium and is related to Planobacterium taklimakanense NCTC 13490 (96.1%, 16S rRNA gene sequence similarity).

Transplantable stem cell nanobridge scaffolds for accelerating articular cartilage regeneration.

Microfracture technique for treating articular cartilage defects usually has poor clinical outcomes due to critical heterogeneity and extremely limited in quality. To improve the effects of current surgical technique (i.e.