Publications by authors named "Sungkuk Hong"
Background: Tissue clearing enables deep imaging in various tissues by increasing the transparency of tissues, but there were limitations of immunostaining of the large-volume tissues such as the whole brain.
Methods: Here, we cleared and immune-stained whole mouse brain tissues using a novel clearing technique termed high-speed clearing and high-resolution staining (HCHS). We observed neural structures within the cleared brains using both a confocal microscope and a light-sheet fluorescence microscope (LSFM).
Article Synopsis
- - Blood vessels have a complex 3D structure that's difficult to analyze with traditional methods, which often damage their topological arrangement.
- - This study used tissue optical clearing techniques combined with light-sheet and confocal microscopy to create high-resolution 3D images of rat brain vasculature after inducing a temporary stroke.
- - Findings indicated that the ischemic area showed reduced blood vessel diameter and density, along with notable neuronal loss and increased astrocyte activity, highlighting the effectiveness of these methods for studying cerebrovascular and neuronal changes in brain disorders.
Article Synopsis
- Vitamin D3 up-regulated protein 1 (VDUP1) acts as a growth suppressor, preventing tumor cell growth and the progression of the cell cycle when it's highly expressed.
- Previous research showed that mice lacking VDUP1 (VDUP1 KO) had faster liver regeneration due to better management of cell cycle regulators.
- In this study, VDUP1 KO mice displayed earlier and stronger activation of key proteins involved in liver regeneration (NF-κB, JNK, and STAT-3) following partial liver removal, suggesting that VDUP1 is crucial for starting the liver regeneration process.