Publications by authors named "Sung Min Bae"

Chalcones are recognized for their diverse pharmacological properties, including anti-inflammatory and anti-melanogenic effects. However, studies on 2'-hydroxy-2-methoxychalcone derivatives remain limited. This study investigated the anti-inflammatory and melanin synthesis-inhibitory effects of three derivatives: 2'-hydroxy-2,4-dimethoxychalcone (2,4-DMC), 2'-hydroxy-2,5'-dimethoxychalcone (2,5'-DMC), and 2'-hydroxy-2,6'-dimethoxychalcone (2,6'-DMC).

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As PCV2d infection has been continuously reported in swine farms in which pigs were vaccinated with PCV2a- or 2d-based vaccines, we attempted to develop a novel vaccine using a PCV2d-based capsid to enhance its protective efficacy. In this study, recombinant virus-like particles (VLPs) of rPCV2a, rPCV2b and rPCV2d were synthesized from the capsid proteins of PCV2a, PCV2b and PCV2d field isolates, respectively. A cross-neutralization assay between the VLPs induced antisera and the field isolates demonstrated the broad cross-neutralizing activities of the rPCV2d-induced antisera.

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Anemia is a frequent complication of chronic kidney disease (CKD) that causes an increase in morbidity and mortality and accelerates the rate of disease progression. Treatment with recombinant human erythropoietin (rhEPO) is a major breakthrough in the therapy of renal anemia. HM10760A, a long-acting EPO, has been developed as a treatment for anemia in CKD patients.

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  • - It is commonly believed that the gene ORF1629 is crucial for baculovirus replication in insect cells, leading to the development of recombinant viruses through a baculovirus expression system.
  • - Researchers discovered that a bacmid (a modified baculovirus) could still proliferate in insect cells even when it lacked a complete ORF1629, indicating that the gene is not strictly necessary for replication.
  • - While ORF1629 is not essential, its absence resulted in slower growth rates and lower yields of the virus, along with larger but fewer occlusion bodies, affecting the overall production efficiency of baculoviruses like AcMNPV and BmNPV.
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  • The baculovirus expression system (BES) is effective for producing tough vertebrate proteins, and Ssp DnaB mini-intein serves as a beneficial fusion partner that can self-cleave under controlled conditions.
  • Recombinant viruses were created to express multiple proteins fused to the mini-intein, resulting in successful intracellular cleavage during infection, although the timing varied with each protein.
  • Overall, using Ssp DnaB mini-intein significantly boosted protein production, helping to counterbalance the loss from self-cleavage, and demonstrates its potential for easier purification and enhanced yields in BES applications.
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Mamestra brassicae nucleopolyhedrovirus-K1 (MabrNPV-K1) was isolated from naturally infected M. brassicae (Lepidoptera: Noctuidae) larvae in Korea. The full genome sequences of MabrNPV-K1 were determined, analysed and compared to those of other baculoviruses.

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To enhance the production efficiency of foreign protein in baculovirus expression systems, the effects of polyhedrin fragments were investigated by fusion expressing them with the enhanced green fluorescent protein (EGFP). Recombinant viruses were generated to express EGFP fused with polyhedrin fragments based on the previously reported minimal region for self-assembly and the KRKK nuclear localization signal (NLS). Fusion expressions with polyhedrin amino acids 19 to 110 and 32 to 110 lead to localization of recombinant protein into the nucleus and mediate its assembly.

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Insulin conjugates in which the B1Phe residue has been chemically modified often exhibit a reduced tendency to associate into hexamers due to weakened interactions between subunits. The purpose of this study was to prepare a hexamer formulation for such insulin conjugates by using Co(III) as a coordinating metal ion. PEGylated insulin in which monomethoxypoly(ethylene glycol) (mPEG, M(r) 5000 or 20,000) had been site-specifically attached to B1Phe was chosen as a model conjugate.

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  • - Monochamus saltuarius is a pest that spreads pine wilt disease, causing significant harm to pine trees in Korea.
  • - A fungus, identified as Beauveria bassiana and named MsW1, was isolated from dead M. saltuarius and showed potential as a biocontrol agent.
  • - Virulence tests indicated that B. bassiana MsW1 resulted in 100% mortality of M. saltuarius adults and larvae after 11 and 13 days, respectively.
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