NeoAI 1.0: Machine learning-based paradigm for prediction of neonatal and infant risk of death.

Background: The Neonatal mortality rate in the United States is 3.8 deaths per 1000 live births, which is comparably higher than other nations.

Purpose: The aim of the proposed study is to design and develop Artificial Intelligence (AI) models (NeoAI 1.

Protein kinase Cζ phosphorylates occludin and promotes assembly of epithelial tight junctions.

Protein kinases play an important role in the regulation of epithelial tight junctions. In the present study, we investigated the role of PKCζ (protein kinase Cζ) in tight junction regulation in Caco-2 and MDCK (Madin-Darby canine kidney) cell monolayers. Inhibition of PKCζ by a specific PKCζ pseudosubstrate peptide results in redistribution of occludin and ZO-1 (zona occludens 1) from the intercellular junctions and disruption of barrier function without affecting cell viability.