Publications by authors named "Sundeep Chakladar"

Apicomplexan parasites use Ca-regulated exocytosis to secrete essential virulence factors from specialized organelles called micronemes. Ca-dependent protein kinases (CDPKs) are required for microneme exocytosis; however, the molecular events that regulate trafficking and fusion of micronemes with the plasma membrane remain unresolved. Here, we combine sub-minute resolution phosphoproteomics and bio-orthogonal labeling of kinase substrates in to identify 163 proteins phosphorylated in a CDPK1-dependent manner.

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Successful infection strategies must balance pathogen amplification and persistence. In the obligate intracellular parasite Toxoplasma gondii this is accomplished through differentiation into dedicated cyst-forming chronic stages that avoid clearance by the host immune system. The transcription factor BFD1 is both necessary and sufficient for stage conversion; however, its regulation is not understood.

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Apicomplexan parasites use Ca-regulated exocytosis to secrete essential virulence factors from specialized organelles called micronemes. Ca-dependent protein kinases (CDPKs) are required for microneme exocytosis; however, the molecular events that regulate trafficking and fusion of micronemes with the plasma membrane remain unresolved. Here, we combine sub-minute resolution phosphoproteomics and bio-orthogonal labeling of kinase substrates in to identify 163 proteins phosphorylated in a CDPK1-dependent manner.

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