The mechanism of AMPA receptor subunit GluR1 in electroacupuncture treatment of acute spinal cord injury in rats.

Glutamate excitotoxicity plays a role in spinal cord injury (SCI). This study aimed to explore whether electroacupuncture (EA) improved the functional recovery of spinal cord anterior horn neurons of rats with acute SCI by regulating the GluR1 AMPA subunit in the SCI area. Eighty Sprague-Dawley rats were randomly divided into 5 groups: sham operation, model, AMPA antagonist (DNQX), EA and DNQX + EA group (n = 16/group).

The mechanism of AMPA receptor subunit GluR1 in electroacupuncture treatment of acute spinal cord injury in rats.

Glutamate excitotoxicity plays a role in spinal cord injury (SCI). This study aimed to explore whether electroacupuncture (EA) improved the functional recovery of spinal cord anterior horn neurons of rats with acute SCI by regulating the GluR1 AMPA subunit in the SCI area. Eighty Sprague-Dawley rats were randomly divided into 5 groups: sham operation, model, AMPA antagonist (DNQX), EA and DNQX+EA group (n=16/group).

Fluorometric determination of zinc(II) by using DNAzyme-modified magnetic microbeads.

A fluorometric assay for zinc ion is described that relies (a) on the use of an isothermal cycle to amplify the fluorescence signal, and (b) of magnetic beads (MBs) to completely remove unreacted DNA detection probes. Biotin and fluorophore-labeled substrate (Zn-Sub) strands acting as detection probes were first assembled on MBs. Next, Zn(II)-specific DNAzyme (Zn-Enz) strands were hybridized with the Zn-Sub strands.