Fish-Bite structure by three-dimensional hydrogen-bond acceptor: IR spectroscopy of pyrrole and N-methylpyrrole binary clusters.

The N-H...

In vivo tissue response and degradation behavior of PLLA and stereocomplexed PLA nanofibers.

Biocompatibility of PLLA and stereocomplexed PLA nanofibers was evaluated by subcutaneous implantation in rats for 4-12 weeks. Characterization of the nanofibers was performed by GPC, SEM, wide-angle X-ray diffraction, and optical microscopy of hematoxylin-eosin stained ultrathin sections of explanted nanofibers. Stereocomplexed PLA nanofiber showed slower degradation than PLLA nanofiber and thus retained their shape after prolonged implantation.

Scaffolds from electrospun polyhydroxyalkanoate copolymers: fabrication, characterization, bioabsorption and tissue response.

Polyhydroxyalkanoate (PHA) copolymers of poly[(R)-3-hydroxybutyrate-co-5mol%-(R)-3-hydroxyhexanoate], poly[(R)-3-hydroxybutyrate-co-7mol%-4-hydroxybutyrate] and poly[(R)-3-hydroxybutyrate-co-97mol%-4-hydroxybutyrate] were electrospun to fabricate scaffolds with enhanced biocompatibility and bioabsorption. Subcutaneous implantation of the fibers in rats was performed to investigate their bioabsorption behavior and tissue response. The fibers before and after the in vivo experiments were characterized using gel permeation chromatography, scanning electron microscopy, X-ray diffraction and tensile test.

Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces.

Primary rat hepatocytes were co-cultured with bone marrow cells (BMCs) in a tissue culture-treated culture well (TCW) or a collagen-coated culture well (CCW). Although a medium containing serum was used, the co-cultured cells formed spheroids on the TCW within 2 days, and ammonia metabolism and albumin secretion activities were well maintained for 3 weeks. The co-cultured cells formed a monolayer on the CCW within 2 days, and liver functions were maintained for 3 weeks.