Pulsed Electric Fields Promote Liposome Buddings.

Liposomes have been a useful tool to analyze membrane behavior. Various studies have attempted to induce biological activities, for example, buddings, divisions, and endocytosis, on liposomes, focusing on lipid rafts that move along electric fields. Liposomes consisting of soybean lecithin, phosphatidylcholine, and cholesterol were prepared, with inner and outer liquid conductivities of 0.

Author Correction: 1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation.

Numerous theoretical studies have been conducted on the effects of high-voltage electric fields on proteins, but few have produced experimental evidence. To acquire experimental data for the amyloid disassemble theory, we exposed transthyretin aggregates to 1,000 ns 1.26 MV/cm pulsed electric fields (PEFs) to promote transthyretin degradation.

Intense Pulsed Electric Fields Denature Urease Protein.

This article describes the effects of nanosecond pulsed electric fields (nsPEFs) on the structure and enzyme activity of three types of proteins. Intense (up to 300 kV/cm) 5-ns-long electrical pulses were applied for 500 times at 3 Hz to solutions of lysozyme, albumin, and urease. We analyzed covalent bonds (peptide bonds and disulfide bonds) of lysozyme and albumin, and also the tertiary and quaternary structures of urease as well as urease activity.

Variations of Intracellular Ca Mobilization Initiated by Nanosecond and Microsecond Electrical Pulses in HeLa Cells.

Goal: Herein, the variations in transient Ca mobilizations in HeLa cells exposed to a single, non-thermal pulsed electric field (PEF) are described.

Methods: Three PEF waveforms categorized by pulse duration and intensity were used to deduce the kinetics involved in Ca mobilization. A fast microscopic fluorescent imaging system and a fluorescent molecular probe were used to observe transient intracellular Ca mobilization after pulse exposure.

Spatio-temporal dynamics of calcium electrotransfer during cell membrane permeabilization.

Pulsed electric fields (PEFs) are applied as physical stimuli for DNA/drug delivery, cancer therapy, gene transformation, and microorganism eradication. Meanwhile, calcium electrotransfer offers an interesting approach to treat cancer, as it induces cell death easier in malignant cells than in normal cells. Here, we study the spatial and temporal cellular responses to 10 μs duration PEFs; by observing real-time, the uptake of extracellular calcium through the cell membrane.

Activation of the JNK pathway by nanosecond pulsed electric fields.

Nanosecond pulsed electric fields (nsPEFs) are increasingly recognized as a novel and unique tool in various life science fields, including electroporation and cancer therapy, although their mode of action in cells remains largely unclear. Here, we show that nsPEFs induce strong and transient activation of a signaling pathway involving c-Jun N-terminal kinase (JNK). Application of nsPEFs to HeLa S3 cells rapidly induced phosphorylation of JNK1 and MKK4, which is located immediately upstream of JNK in this signaling pathway.