Antioxidant activity of different parts of Dolsan Leaf Mustard.

This study aimed to investigate the sinigrin content and antioxidant properties of Dolsan mustard seeds (DMS) and Dolsan leaf mustard (DLM) extracted with 50% acetonitrile. The extracted substances were fractionated by HPLC using a preparative ODS column and then applied to measure sinigirn, total polyphenol (TP), and total flavonoid (TF) contents and antioxidant activities using 2,2-azino-bis(3-ethyl-benzothizoline-6-sulfonic acid) (ABTS), electron donating ability (EDA), and ferric reducing antioxidant power (FRAP) assays. The contents of sinigrin (53.

Novel embryoid body-based method to derive mesenchymal stem cells from human embryonic stem cells.

Application of human embryonic stem cells (hESCs) to stem-cell therapy is not feasible because of the risk of tumorigenicity and rejection. In contrast, human mesenchymal stem cells (hMSCs) are free from the risk of tumorigenicity and also have immune privilege. However, hMSCs obtained from adults have infinite variety in terms of the biological characteristics and functionality.

Efficient gene delivery in differentiated human embryonic stem cells.

Human embryonic stem (hES) cells are capable of differentiating into pluralistic cell types, however, spontaneous differentiation generally gives rise to a limited number of specific differentiated cell types and a large degree of cell heterogeneity. In an effort to increase the efficiency of specified hES cell differentiation, we performed a series of transient transfection of hES cells with EGFP expression vectors driven by different promoter systems, including human cellular polypeptide chain elongation factor 1 alpha (hEF1alpha), human cytomegalo-virus, and chicken beta-actin. All these promoters were found to lead reporter gene expression in undifferentiated hES cells, but very few drug-selectable transfectants were obtained and failed to maintain stable expression of the transgene with either chemical or electroporation methods.

Chromosomal constitution of embryos derived from tripronuclear zygotes studied by fluorescence in situ hybridization using probes for chromosomes 4, 13, 18, 21, X, and Y.

This study was designed to assess the chromosomal constitution and segregation patterns of cleaving embryos derived from tripronuclear zygotes. Thirty-two embryos obtained from 19 conventional IVF patients were analyzed by fluorescence in situ hybridization (FISH) using probes for chromosomes 4, 13, 18, 21, X, and Y. Sixteen embryos (50.