Genome-Wide Identification and Expression Analysis of HSP70 Gene Family Under High-Temperature Stress in Lettuce ( L.).

The heat shock protein 70 (HSP70) family plays an important role in the growth and development of lettuce and in the defense response to high-temperature stress; however, its bioinformatics analysis in lettuce has been extremely limited. Genome-wide bioinformatics analysis methods such as chromosome location, phylogenetic relationships, gene structure, collinearity analysis, and promoter analysis were performed in the gene family, and the expression patterns in response to high-temperature stress were analyzed. The mechanism of in heat resistance in lettuce was studied by virus-induced gene silencing (VIGS) and transient overexpression techniques.

Characterization and Transcriptional Regulation of the 2-Ketogluconate Utilization Operon in .

JUIM01 is an industrial 2-keto-d-gluconate (2KGA)-producing strain. However, its regulation mechanism of 2KGA metabolism remains to be clarified. Among other reported species, the 2-ketogluconate utilization operon ( operon) plays key roles in 2KGA catabolism.

Lithium salt-derived artificial near-surface reconfiguration to stabilize high-voltage LiCoO.

Pushing the limit of the charging cut-off voltage inevitably leads to the instability of bulk and interfacial structures. Herein, one-step dual-modified LiCoO (LCO) is achieved by thermodynamic decomposition of lithiuim salts on the surface, featuring F-doped bulk and LiF & LiBO coating layers. Notably, such artificial near-surface reconfiguration effectively suppresses Co dissolution, structural deconstruction and electrolyte side reactions during repeated lithiation/delithiation processes.

Transcriptomic analysis of the HPT axis in a model of oligoasthenozoospermia induced by Adenine in rats.

Male infertility is most commonly caused by oligozoospermia, and its pathogenesis is still poorly understood at the molecular level. This study used RNA sequencing (RNA-Seq) technology to identify candidate genes and regulatory pathways that regulate semen quality in the hypothalamic, pituitary, and testicular tissues of healthy rats and Adenine-induced oligozoospermia model rats. Semen quality testing and histological analysis of testicular tissues were performed on both groups of rats.

Fungal β-1, 3-glucanosyltransferases: A comprehensive review on classification, catalytic mechanism and functional role.

β-1,3-Glucans form the major carbohydrate component of fungal cell walls, playing a vital role in cell viability, stress response, virulence, and even healthy functions such as immuno-enhancement. The elongation and branching of β-1,3-glucans is a mystery. More evidence proved the β-1, 3-glucantransferases belonging to GH72 or GH17 family to branch and remodel the synthesized linear β-1, 3-glucan chain by cleaving its internal β-1, 3-linkage and transfer the cleaved fragment to the nonreducing end of another β-1, 3-glucan acceptor.

Enzymatic and structural properties of a novel oxalate decarboxylase BsOxdC from Bacillus safensis and its potential pH-dependent catalytic mechanism.

Oxalate decarboxylase converts oxalate to formate and CO without requiring organic cofactors, making it biotechnologically relevant for applications in food, agriculture, and diagnostics. Its activity is highly dependent on pH; however, the pH-dependent catalytic mechanism remains poorly understood. This study identified a novel oxalate decarboxylase, BsOxdC, from Bacillus safensis and investigated its catalytic properties through heterologous expression and enzymatic assays.

Divergent responses of an armored and an unarmored dinoflagellate to ocean acidification.

Dinoflagellates, both armored and unarmored, with distinct cell wall difference, are being affected by elevated CO-induced ocean acidification (OA). However, their specific responses to OA are not well understood. In this study, we investigated the physiological and molecular response of the armored species Prorocentrum obtusidens and the unarmored species Karenia mikimotoi to OA over a 28-day period.

Review of Foam with Novel CO-Soluble Surfactants for Improved Mobility Control in Tight Oil Reservoirs.

CO-soluble surfactant foam systems have gained significant attention for their potential to enhance oil recovery, particularly in tight oil reservoirs where conventional water-soluble surfactants face challenges such as poor injectability and high reservoir sensitivity. This review provides a comprehensive explanation of the basic theory of CO-soluble surfactant foam, its mechanism in enhanced oil recovery (EOR), and the classification and application of various CO-soluble surfactants. The application of these surfactants in tight oil reservoirs, where low permeability and high water sensitivity limit traditional methods, is highlighted as a promising solution to improve CO mobility control and increase oil recovery.

Quantitative assessments of white matter hyperintensities and plasma biomarkers can predict cognitive impairment and cerebral microbleeds in cerebral small vessel disease patients.

The objective of this study is to examine the efficacy of magnetic resonance imaging (MRI) features and peripheral blood biomarkers in assessing cognitive function in patients with cerebral small vessel disease (CSVD). A total of 58 CSVD patients were recruited. Six features of white matter hyperintensities (WMHs) were derived from MRI scans.

The Functional Characterization of the 6-Phosphogluconate Dehydratase Operon in 2-Ketogluconic Acid Industrial Producing Strain JUIM01.

Genus bacteria mainly consume glucose through the Entner-Doudoroff (ED) route due to a lack of a functional Embden-Meyerhof-Parnas (EMP) pathway. In the present study, a 6-phosphogluconate dehydratase () operon in the ED route was well investigated to find its structural characteristics and roles in the regulation of glucose consumption and 2-ketogluconic acid (2KGA) metabolism in the industrial 2KGA-producer JUIM01. The operon contained four structural genes of , , , and , encoding 6-PG dehydratase Edd, glucokinase Glk, response regulatory factor GltR, and histidine kinase GtrS, respectively.

Boosting the Photoresponse of Azobenzene Single-Molecule Junctions via Mechanical Interlock and Dynamic Anchor.

As the most classic photoisomerization system, azobenzene has been widely utilized as a building unit in various photoswitching applications. However, attempts to build azobenzene-based single-molecule photoswitches have met with limited success, giving low on/off ratios. Herein, we demonstrate two designs of azobenzene-based photoresponsive single-molecule junctions, based on mechanically interlocked diazocine and azobenzene-based dynamic anchors, respectively.

Sediment accretion and nutrient enrichment enhance the growth and vegetative propagation of growing within a stand.

Sediment accretion (burial) and nutrient enrichment may exert a synergistic influence on the growth and distribution of macrophytes in floodplain wetlands; however, this phenomenon has rarely been examined. In this study, we investigated the effects of sediment accretion and nutrient enrichment on the growth and vegetative propagation of within a stand (one ramet within 25 C ramets) using a factorial sediment burial (0, 3, and 6 cm) and nutrient addition (low, medium, and high) experimental design. High sediment burial (6 cm) without nutrient addition decreased the aboveground and total biomass of but did not affect , indicating that is more tolerant to sediment burial than .

Integrated Omnidirectional Design of Non-Volatile Solid Additive Enables Binary Organic Solar Cells with Efficiency Exceeding 19.5 .

Solid additives have drawn great attention due to their numerous appealing benefits in enhancing the power conversion efficiencies (PCEs) of organic solar cells (OSCs). To date, various strategies have been reported for the selection or design of non-volatile solid additives. However, the lack of a general design/evaluation principles for developing non-volatile solid additives often results in individual solid additives offering only one or two efficiency-boosting attributes.

Oxidative Cleavage of Aromatic C-O Linkages by Oxoammonium Salts.

Oxidative cleavage of aromatic C(sp)-O bond is important to the conversion of biomass and plastic wastes into value-added chemicals. Here we put forward the oxidative cleavage of para-C-O bonds in phenolic compounds in use of oxoammonium salts as oxidant and water as the oxygen source. The mechanism is that oxoammonium cation activates water to form hydroxy-oxoammonium adduct and thus realizes the ipso-substitution of 4-alkoxyphenol, which is proved by substituent effect, isotope labelling experiments, and kinetic analysis.

Deubiquitinase USP5 regulates RIPK1 driven pyroptosis in response to myocardial ischemic reperfusion injury.

Background: Gasdermin D (GSDMD) mediated pyroptosis plays a significant role in the pathophysiology of myocardial ischemia/reperfusion (I/R) injury. However, the precise mechanisms regulating pyroptosis remain unclear. In the study, we aimed to investigate the underlying mechanism of pyroptosis in myocardial I/R injury.

Development and application of an artificial intelligence-assisted endoscopy system for diagnosis of Helicobacter pylori infection: a multicenter randomized controlled study.

Background: The early diagnosis and treatment of Heliobacter pylori (H.pylori) gastrointestinal infection provide significant benefits to patients. We constructed a convolutional neural network (CNN) model based on an endoscopic system to diagnose H.

Roles of the NR2F Family in the Development, Disease, and Cancer of the Lung.

The NR2F family, including NR2F1, NR2F2, and NR2F6, belongs to the nuclear receptor superfamily. NR2F family members function as transcription factors and play essential roles in the development of multiple organs or tissues in mammals, including the central nervous system, veins and arteries, kidneys, uterus, and vasculature. In the central nervous system, NR2F1/2 coordinate with each other to regulate the development of specific brain subregions or cell types.

TOB1 inhibits the gastric cancer progression by focal adhesion pathway and ERK pathway based on transcriptional and metabolic sequencing.

Gastric cancer is one of the most malignant digestive tract tumors worldwide and its progression is associated with gene expression and metabolic alteration. We revealed that the gastric cancer patients with lower expression level of TOB1 exhibited poorer overall survivals according to the data in Kaplan-Meier Plotter. The unphosphorylated TOB1 protein which is effective expressed lower in gastric cancer cells.

Effects of fermentation conditions on molecular weight, production, and physicochemical properties of gellan gum.

Gellan gum has been widely used in many industries due to its excellent physical properties. In this study, the effects of different fermentation conditions on molecular weight and production of gellan gum were analyzed, and the optimized fermentation conditions for a high molecular weight gellan gum (H-GG: 6.42 × 10 Da) were obtained, which increased the molecular weight and yield of gellan gum by 201.

MEF2A, a gene associated with mitochondrial biogenesis, promotes drug resistance in gastric cancer.

Chemotherapeutic resistance is a major obstacle to the effectiveness of cisplatin-based chemotherapy for gastric cancer (GC), leading to treatment failure and poor survival rates. However, the underlying mechanisms are not fully understood. Our study demonstrated that the transcription factor myocyte enhancer factor 2A (MEF2A) plays a role in chemotherapeutic drug resistance by regulating the transcription of PGC1α and KEAP1, promoting mitochondrial biogenesis.