Glucagon-like peptide-1 receptor signaling activation in alveolar type II cells enhances lung development in neonatal rats exposed to hyperoxia.

Background: Many studies have reported the important role of glucagon-like peptide-1 receptor (GLP-1R) in regulating glucose homeostasis. However, in addition to the pancreas, GLP-1R is distributed in organs such as the lungs. A few researches have reported the mechanism of action of GLP-1R in acute and chronic lung diseases.

The Effects of Sika Deer Antler Peptides on 3T3-L1 Preadipocytes and C57BL/6 Mice via Activating AMPK Signaling and Gut Microbiota.

(1) Background: To explore the anti-obesity effects and mechanisms of sika deer velvet antler peptides (sVAP) on 3T3-L1 preadipocytes and in high-fat diet (HFD)-induced obese mice. (2) Methods: sVAP fractions of different molecular weights were obtained via enzymatic hydrolysis and ultrafiltration. Their anti-lipid effects on 3T3-L1 cells were assessed with Oil Red O staining.

Preparation of polyvinyl alcohol/sodium alginate/Artemisia sphaerocephala Krasch gum hydrogels with excellent water absorption and its application in the preservation of Lateolabrax Japonicus fillets.

The natural high molecular polysaccharide Artemisia sphaerocephala Krasch gum (ASKG) was introduced into Polyvinyl alcohol (PVA) and Sodium alginate (SA), and the hydrogels with excellent absorbent properties were prepared by crosslinking method. The hydrogel form porous network structures through esterification of CA with PVA and ASKG, and chelation of Ca and SA. The water absorption, water retention, porosity and thermal stability of hydrogels are improved by ASKG.

Peptides from Dalian Stichopus japonicus: Antioxidant Activity and Melanogenesis Inhibition In Vitro Cell Models and In Vivo Zebrafish Models Guided by Molecular Docking Screening.

This article aims to reveal the optimal peptide segment with antioxidant activity from Dalian Stichopus japonicus, investigate its anti-melanogenesis effect, and elucidate its mechanisms of action both in vitro and in vivo. The best antioxidant alcalase hydrolysates, identified by the previous screening of proteases, was isolated by ultrafiltration; it was found that the components with a molecular weight of ≤ 3 kDa exhibited the best activity. The chemical components were characterized using LC-MS/MS.

Nanoscale CaO-Loaded Surface-Engineered Iodine-125 Seed with Sustained Self-Oxygenation for Sensitized Tumor Brachytherapy.

Iodine-125 (I) brachytherapy (BT) is renowned for its precision and effectiveness in delivering localized radiation doses to solid tumors. However, the therapeutic efficacy of traditionalI seed is often limited due to the inherent and acquired radioresistance. Based on the importance of tumor hypoxia in radioresistance, a novel "in situ oxygen-supplement" surface-modified radioactive I seed (I@TNT-CaO) is designed and constructed to overcome hypoxia-induced radioresistance in tumor BT.

Isolation and identification of a subtype C avian metapneumovirus in chickens in Jiangsu, China.

Avian metapneumovirus (aMPV) is a viral pathogen that mainly causes respiratory signs and drops in egg production in turkeys, chickens, and ducks. Here, an aMPV subgroup C (aMPV/C) strain, designated GX22-01, was isolated and identified from severe respiratory disease in broiler breeder chickens in 2022 in Jiangsu, China, as evidenced by indirect immunofluorescence and western blotting using specific anti-viral protein antibodies and by sequence analysis of viral nucleoprotein (N) gene. N gene sequencing indicated that the GX22-01 strain shares a high identity (94.

Glycyrrhizin Alleviates the Damage Caused by Zearalenone and Protects the Glandular Stomach of Chickens.

Zearalenone (ZEA) is a kind of mycotoxin that widely contaminates food and feed and poses a threat to poultry farming. As a natural extract, glycyrrhizin acid (GA) has antioxidant, antibacterial, and anti-inflammatory effects. Although studies have revealed the toxic effects of ZEA on the liver, the mechanism by which GA reduces ZEA's toxic protective glandular stomach remains unclear.

Comprehensive Molecular Profiling of Metastatic Pancreatic Adenocarcinomas.

Background: Pancreatic ductal adenocarcinoma (PDAC) is often diagnosed late, with an extremely poor prognosis. Treatment options like surgery, radiation, and chemotherapy are rarely curative. Tumor progression from primary to metastatic PDAC remains poorly understood at the molecular level.

Localized Alkaline Microenvironments Enhanced upon Tip Effects for Efficient Antibacterial Applications.

The electrochemical disinfection mechanism primarily relies on a high-voltage pulsed electric field and electrocatalytic generation of highly oxidative radicals but suffers from shortcomings of either high voltages (dozens of kV) or significant gas supply. This study proposes an in situ electrocatalytic sterilization strategy that forms localized alkaline microenvironments in neutral electrolytes under a constant current of -12 mA at a relatively lower voltage (-0.5 V vs.

Acceptance and commitment therapy for nurses' sleep, rumination, psychological flexibility, and it's neural mechanism: A randomized controlled fNIRS study.

Given nurses often face sleep problems, this study compares two internet-delivered Acceptance and Commitment Therapy (ACT) interventions to improve sleep quality (SQ), psychological flexibility (PF), reduce rumination, and explore neural mechanisms. 477 nurses were randomly assigned to ACT linear psychotherapy model (LINEAR), ACT loop psychotherapy model (LOOP) and wait-list group. SQ, rumination, and PF were assessed with questionnaires.

Antioxidant activities of three enzymatic hydrolysates from on HaCaT cells and zebrafish .

To explore the antioxidant activity of enzymatic hydrolysates of from Dalian and preliminarily elucidate their mechanisms of action both and . Samples were hydrolysed using alcalase, protamex, and neutrase. 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays showed that the alcalase hydrolysate had the highest antioxidant activity, with IC values of 4.

Quantification of Vascular Remodeling and Sinusoidal Capillarization to Assess Liver Fibrosis with Photoacoustic Imaging.

Background Photoacoustic microscopy (PAM) can be used to detect strong absorption from endogenous and exogenous contrast material, making it promising for detailed structural and functional imaging of hepatic sinusoids, including dynamic visualization of permeability. Purpose To evaluate whether PAM-based quantitative parameters of liver function and integrity (lacunarity, blood oxygen saturation [Sao], and Evans blue [EB] permeability) are associated with histopathologic indexes of fibrosis in a mouse model. Materials and Methods Between October 2022 and July 2023, a total of 35 male C57BL/6 mice were included in this study and received intraperitoneal injection of carbon tetrachloride to establish mouse models of progressive liver fibrosis, with seven mice in each group.

[Construction and optimization of 1, 4-butanediamine biosensor based on transcriptional regulator PuuR].

Biosensors have become powerful tools for real-time monitoring of specific small molecules and precise control of gene expression in biological systems. High-throughput sensors for 1, 4-butanediamine biosynthesis can greatly improve the screening efficiency of high-yielding 1, 4-butanediamine strains. However, the strategies for adapting the characteristics of biosensors are still rarely studied, which limits the applicability of 1, 4-butanediamine biosensors.

Integrating machine learning, suspect and nontarget screening reveal the interpretable fates of micropollutants and their transformation products in sludge.

Activated sludge enriches vast amounts of micropollutants (MPs) when wastewater is treated, posing potential environmental risks. While standard methods typically focus on target analysis of known compounds, the identity, structure, and concentration of transformation products (TPs) of MPs remain less understood. Here, we employed a novel approach that integrates machine learning for the quantification of nontarget TPs with advanced target, suspect, and nontarget screening strategies.

Changes in Volatile Compounds and Sensory Properties of Chicken with During the Pressure-Cooking Process.

Chicken with prepared via pressure cooking is a traditional Chinese delicacy with great potential for food development. Optimizing its cooking time is crucial. In this study, chicken and were pressure-cooked for different amounts of time (20 min, 25 min, 30 min, 35 min, and 40 min).

A comparative analysis of lumboperitoneal shunt outcomes in patients with post-hemorrhagic and post-traumatic hydrocephalus.

Hydrocephalus, whether arising from post-hemorrhagic or post-traumatic origins, poses significant challenges in clinical management. Lumboperitoneal shunting (LPS) emerges as a viable therapeutic intervention, yet comparative analyses between these etiologies remain scarce. This retrospective study aims to compare the efficacy and safety of LPS placement in patients with post-hemorrhagic (PHH) and post-traumatic hydrocephalus (PTH).

Activated DRP1 promotes mitochondrial fission and induces glycolysis in ATII cells under hyperoxia.

Backgroud: Recent studies have reported mitochondrial damage and metabolic dysregulation in BPD, but the changes in mitochondrial dynamics and glucose metabolic reprogramming in ATII cells and their regulatory relationship have not been reported.

Methods: Neonatal rats in this study were divided into model (FIO2:85%) and control (FIO2: 21%) groups. Lung tissues were extracted at 3, 7, 10 and 14 postnatal days and then conducted HE staining for histopathological observation.

The polycomb protein complex interacts with GATA-6/PPARα to inhibit α-MHC expression.

Transcription factors collaborate with epigenetic regulatory factors to orchestrate cardiac differentiation for heart development, but the underlying mechanism is not fully understood. Here, we report that GATA-6 induces cardiac differentiation but peroxisome proliferator-activated receptor α (PPARα) reverses GATA-6-induced cardiac differentiation, possibly because GATA-6/PPARα recruits the polycomb protein complex containing EZH2/Ring1b/BMI1 to the promoter of the cardiac-specific α-myosin heavy chain (α-MHC) gene and suppresses α-MHC expression, which ultimately inhibits cardiac differentiation. Furthermore, Ring1b ubiquitylates PPARα and GATA-6.

TFAP2C-mediated transcriptional activation of STEAP3 promotes lung squamous cell carcinoma progression by regulating the β-catenin pathway.

Six-transmembrane epithelial antigen of prostate 3 (STEAP3) is associated with the progression of several human malignancies. However, its role in lung squamous cell carcinoma (LUSC) remains unclear. We measured STEAP3 expression in LUSC cell lines and tissues.

Risk factors for diabetic retinopathy in young and middle-aged patients: a retrospective study.

Aims/introduction: To investigate the related risk factors of retinopathy in young and middle-aged diabetic patients in order to improve the prognosis of patients.

Materials And Methods: Using clinical practice data from a cohort study at our two research centers, we developed a bivariate logistic regression model to investigate the frightening risk factors potentially for retinopathy in young and middle-aged patients with diabetes, including diabetes type, physical activity level, treatment-related characteristics and laboratory tests.

Results: A total of 453 patients with diabetes were investigated, 197 (43.

Associations of elevated cardiac biomarkers with hyperuricemia and mortality in US adults without prevalent cardiovascular disease.

Background: NT-proBNP (N-terminal pro-B-type natriuretic peptide), high-sensitivity cardiac troponin T (hs-troponin T), and high-sensitivity cardiac troponin I (hs-troponin I) have been widely recognized as significant cardiac biomarkers, and are increasingly being recommended for early risk identification in cardiovascular high-risk populations. The aim of our study was to evaluate the prevalence of elevated cardiac biomarkers (NT-proBNP, hs-troponin T, hs-troponin I) and their association with the risk of hyperuricemia in the general US adults without known cardiovascular disease. We further studied whether elevated cardiac biomarkers are associated with an increased risk of all-cause and cardiovascular mortality in individuals with or without hyperuricemia.

Effect of SARS-CoV-2 infection on liver function in patients with hepatitis B.

Objective: To investigate the impact of SARS-CoV-2 infection on liver function and prognosis in patients with HBV infection.

Methods: A total of 154 HBV-positive patients (HBV ( +) group) and 154 HBV-negative patients (HBV (-) group) diagnosed with COVID-19 at Taizhou Hospital between December 10, 2022, and January 31, 2023, were included in this study. Clinical characteristics, treatment, and laboratory findings were collected from patients at three time points: before (T1), during (T2), and at the time of discharge (T3) from SARS-CoV-2 infection.

Baicalein disrupts the KEAP1-NRF2 interaction to alleviate oxidative stress injury by inhibiting M1 macrophage polarization.

Macrophages are key players in maintaining the balance of tissues and dealing with inflammation, carrying out vital functions specific to different tissues while safeguarding the body against infections. The intricate interplay between oxidative stress and macrophage polarization and how this contributes to pneumonia is not fully understood. Herein, a predominant accumulation of baicalein in lung macrophages of pathogen-infected mice was observed by an alkynyl-modified probe.

Paeoniflorin ameliorates reperfusion injury in H9C2 cells through SIRT1-PINK1/parkin-mediated mitochondrial autophagy.

Myocardial ischemia-reperfusion injury (MIRI) injury is a serious health problem, which can seriously affect the recovery of patients with myocardial infarction and even lead to death. Paeoniflorin (PF) is a potential therapeutic drug to prevent reperfusion injury. However, the mechanism of PF in MIRI is not clear.