Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi007-A) from a patient with Kennedy disease.

Abnormal trinucleotide CAG repeat expansions in exon 1 of the Androgen Receptor (AR) gene has been identified as the cause of Kennedy disease (KD). We generated and characterized a human induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells (PBMC) of a patient with genetically confirmed KD. The pluripotency of these iPSCs was verified by the expression of several pluripotency markers at both RNA and protein levels, as well as their capability to differentiate into all three germ layers.

Engineering an Ionic Aggregation-Induced Luminescence-Labeled Fluorescence Lateral Flow Immunoassay for C-Reactive Protein in Human Plasma.

The surge of lateral flow immunoassays (LFAs) stimulates researchers to explore the novel vibrant aggregation-induced emission luminogen (AIEgen)-doped nanoparticles to improve the accuracy and reliability of LFAs. However, the loading amount of AIEgens currently used for the LFA in microspheres is limited due to their symmetrical large conjugated skeleton structure, which significantly reduces the fluorescence brightness of the signal reporter in the LFA. Herein, an ionic AIEgens with a donor-acceptor type was developed as the signal reporter of the LFA for C-reactive protein (CRP).

Spatiotemporal-controllable ROS-responsive camptothecin nano-bomb for chemo/photo/immunotherapy in triple-negative breast cancer.

Chemotherapy is still one of the major approaches in triple-negative breast cancer (TNBC) treatment. The development of new formulations for classic chemotherapeutic drugs remains interests in studies. Camptothecin (CPT) is powerful antitumor agents in TNBC treatment though its clinic applications are limited by its low water solubility and systemic toxicity.

Manipulating Toughness and Microstructure in Polyelectrolyte Complex Hydrogels with Competitive Surfactant Micelles.

Polyelectrolyte complex (PEC) hydrogels provide a promising strategy to develop a class of physically cross-linked networks characterized by exceptional toughness and self-healing properties. However, the precise control of the microstructure and the enhancement of mechanical properties still pose challenges in the field of PEC hydrogels. Herein, we propose a strategy to manipulate the structure of PEC with competitively charged surfactant micelles, leveraging the spatially confined surface charge and excluded volume effects to overcome coacervation issues associated with the PEC, thus achieving a simple one-step preparation of macroscopically uniform and tough PEC hydrogels.

Pyridazine and pyridazinone compounds in crops protection: a review.

Pyridazine and pyridazinone belong to the same group of six-membered heterocyclic compounds, and both structurally feature two adjacent nitrogen atoms. Pyridazine and pyridazinone derivatives are frequently used as core structures in the development of new green agrochemicals due to their high activity and environmental friendliness, attracting significant attention from researchers in recent years. Over the past 20 years, significant developments have occurred in the field of pyridazine and pyridazinone derivatives, which exhibit insecticidal, fungicidal, herbicidal, antiviral, and plant growth regulating activities.

Carbon Dots-Modified Hollow Mesoporous Photonic Crystal Materials for Sensitivity- and Selectivity-Enhanced Sensing of Chloroform Vapor.

Chloroform and other volatile organic pollutants have garnered widespread attention from the public and researchers, because of their potential harm to the respiratory system, nervous system, skin, and eyes. However, research on chloroform vapor sensing is still in its early stages, primarily due to the lack of specific recognition motif. Here we report a mesoporous photonic crystal sensor incorporating carbon dots-based nanoreceptor (HMSS@CDs-PCs) for enhanced chloroform sensing.

[Transcriptomic differences between the spleens of mice immunized with inactivated antigens of foot-and-mouth disease virus and Senecavirus A].

The aim of this study was to compare the immune responses of C57BL/6 mice immunized with two pathogens, foot-and-mouth disease virus (FMDV) and Senecavirus A (SVA), and to provide clues for revealing the regulatory mechanisms of acquired immunity. Inactivated and purified FMDV and SVA antigens were used to immunize C57BL/6 mice respectively, and the mice immunized with PBS were taken as the control. The percentages of Th1 and Th2 cells in the spleen lymphocytes of mice in each group were analyzed by flow cytometry at 14 and 28 days after immunization.

Novel Nanoliposomes Synergistically Modulated by Sitogluside and Dioscin: Stability, Bioavailability, and Capacity To Alleviate Hyperuricaemia.

Cholesterol (Cho) is commonly used to stabilize nanoliposomes; however, there is controversy on the relationship between Cho and health. In this study, we developed a novel multifunctional nanoliposome utilizing structurally similar sitogluside (SG) and dioscin (Dio) instead of Cho to anchor the phospholipid bilayer and synergistically modulate the membrane properties of the nanoliposome (DPPC or DOPC). The storage and gastrointestinal tract stability experiment demonstrated that the changes of physical and chemical properties, including the significantly reduced size and Dio retention rate of nanoliposomes synergistically modulated by SG and Dio compared to those of SG alone, regulated nanoliposomes.

Laparoscopic Radical Antegrade Modular Pancreatosplenectomy with Portal Vein Reconstruction and Celiac Axis Resection for Pancreatic Neck-Body Cancer.

Background: Laparoscopic radical antegrade modular pancreatosplenectomy combined with celiac axis resection and portal vein reconstruction is a new procedure for the treatment of pancreatic cancer. This surgical technique may offer patients with pancreatic cancer involving the portal vein and celiac axis an opportunity for radical surgical resection. We aim to evaluate the short- and long-term efficacy and describe the surgical details of this technique.

The NFATC2/Nrf2 cascade regulates spinal cord ischemia-reperfusion injury by controlling inflammation, apoptosis and oxidative stress.

Spinal cord ischemia/reperfusion (IR) injury (SCII) can cause major autonomic, sensory, and motor damage and loss. The upregulation of Nrf2, a primary orchestrator of the oxidative stress response, has beneficial effects in SCII. Here, we aimed to uncover a SCII-related transcription factor that is able to elevate Nrf2 expression.

[Association of dietary diversity and anxiety in Chinese people aged 65 years and older in 2018].

Objective: To estimate the prevalence of anxiety and examine the association between the dietary diversity and anxiety among older Chinese adults.

Methods: The data from the 2018 Chinese Longitudinal Healthy Longevity Survey(CLHLS) was used, a nationwide survey with 10 555 adults aged ≥65 years in 23 provinces of China. Dietary diversity score(DDS) was constructed based on 11 items of a food frequency questionnaire.

Innovative strategies to optimise colorectal cancer immunotherapy through molecular mechanism insights.

Background: Colorectal cancer (CRC) is a leading cause of cancer-related deaths globally. The heterogeneity of the tumor microenvironment significantly influences patient prognosis, while the diversity of tumor cells shapes its unique characteristics. A comprehensive analysis of the molecular profile of tumor cells is crucial for identifying novel molecular targets for drug sensitivity analysis and for uncovering the pathophysiological mechanisms underlying CRC.

Advanced characterization of fish skin gelatin-proanthocyanidins covalent and non-covalent composite emulsions for benzyl isothiocyanate delivery.

This research endeavored to engineer robust delivery matrices for bioactives, specifically benzyl isothiocyanate (BITC), by harnessing the synergistic covalent and non-covalent interactions between fish skin gelatin (FSG) and proanthocyanidins (PC) to synthesize novel composite emulsions. The objective was to delineate the influence of these molecular interactions on the emulsion's structural integrity and stability, which are pivotal for the efficacious encapsulation and controlled release of BITC. Employing a suite of analytical techniques, including Fourier transform infrared spectroscopy (FTIR), fluorescence spectroscopy, and contact angle measurements, the study delineated the predominant molecular forces at play within the FSG-PC complex, identifying electrostatic and hydrophobic interactions as the cornerstones of this interaction.

A novel self-assembling peptide nanofiber hydrogel with glucagon-like peptide-1 functionality enhances islet survival to improve islet transplantation outcome in diabetes treatment.

Islet transplantation is a promising therapy for diabetes, yet the limited survival and functionality of transplanted islet grafts hinder optimal outcomes. Glucagon-like peptide-1 (GLP-1), an endogenous hormone, has shown potential to enhance islet survival and function; however, its systemic administration can result in poor localization and undesirable side effects. To address these challenges, we developed a novel peptide-based nanofiber hydrogel incorporating GLP-1 functionality for localized delivery.

IL-33 and soluble ST2 in follicular fluid are associated with premature ovarian insufficiency.

Background: Premature ovarian insufficiency (POI) is a common reproductive disease that is associated with chronic inflammation in ovaries. Interleukin 33 (IL-33) is a pro-inflammatory IL-1 family cytokine, and functions as an alarmin reflecting inflammatory reaction. Our study aimed to investigate levels of IL-33 and its soluble receptor (sST2) in both follicular fluid (FF) and paired serum during different stages of POI, and evaluate their predictive potentials for POI.

Causal relationship between genetically predicted mental disorders and frailty: a bidirectional and multivariable mendelian randomization study.

Background: In observational studies, frailty has been strongly associated with mental disorders. However, the mechanisms underlying the association between frailty and mental disorders remain unclear.

Methods: We conducted a two-sample Mendelian randomization (MR) study to assess the causal relationship between frailty, as measured by the frailty index (FI), and ten common mental disorders.

Assembly of a biomimetic copper-based nanocomplex for alleviating hypoxia to enhance cuproptosis against osteosarcoma and lung metastasis.

Osteosarcoma tissues demonstrated elevated expression of proteins (FDX1 and DLAT) integral to cuproptosis in our preliminary study, indicating the potential effectiveness of anti-tumor strategies predicated on this process. Nevertheless, the overexpression of copper export proteins and the challenge of copper ion penetration may contribute to insufficient local copper ion concentration for inducing cuproptosis. Herein, we engineered a biomimetic copper-elesclomol-polyphenol network for the efficient delivery of copper ions and the copper ionophore elesclomol.

Identification of hub genes, diagnostic model, and immune infiltration in preeclampsia by integrated bioinformatics analysis and machine learning.

Purpose: This study aimed to identify novel biomarkers for preeclampsia (PE) diagnosis by integrating Weighted Gene Co-expression Network Analysis (WGCNA) with machine learning techniques.

Patients And Methods: We obtained the PE dataset GSE25906 from the gene expression omnibus (GEO) database. Analysis of differentially expressed genes (DEGs) and module genes with Limma and Weighted Gene Co-expression Network analysis (WGCNA).

Endocrine disruptor identification and multitoxicity level assessment of organic chemicals: An example of multiple machine learning models.

Endocrine-disrupting chemicals (EDCs) pollution is a major global environmental issue. Assessing the multiple toxic effects of EDCs is key to managing their risks. This study successfully developed an EDCs classification and recognition model based on recursive feature elimination and random forest coupling, which passed external validation.

Lanthanide binding peptide surfactants at air-aqueous interfaces for interfacial separation of rare earth elements.

Rare earth elements (REEs) are critical materials to modern technologies. They are obtained by selective separation from mining feedstocks consisting of mixtures of their trivalent cation. We are developing an all-aqueous, bioinspired, interfacial separation using peptides as amphiphilic molecular extractants.

Intravoxel incoherent motion diffusion-weighted imaging in nasopharyngeal carcinoma: comparison of the turbo spin-echo and echo-planar imaging techniques.

Background: Traditional echo-planar imaging with intravoxel incoherent motion (EPI-IVIM) exhibits significant magnetic susceptibility artifacts and geometric distortions, which limits its application in nasopharyngeal carcinoma (NPC). This study aimed to compare the qualitative and quantitative indicators between turbo spin echo with intravoxel incoherent motion (TSE-IVIM) and EPI-IVIM in patients with NPC and to provide optimal scanning strategies based on the relationships among these indicators.

Methods: A cross-sectional study was conducted between March 2022 and August 2022.

A bismuth oxide-modified copper host achieving bubble-free and stable potassium metal batteries.

Due to the minimal electrochemical oxidation-reduction potential, the potassium (K) metal anode has emerged as a focal in K-ion batteries. However, the reactivity of the K metal anode leads to significant side reactions, particularly gas evolution. Mitigating gas generation from K metal anodes has been a persistent challenge in the field.