Phytochlorin-Based Sonosensitizers Combined with Free-Field Ultrasound for Immune-Sonodynamic Cancer Therapy.

Phytochlorins, a class of plant-derived tetrapyrroles, show great potential as sonosensitizers in sonodynamic therapy (SDT). The development of new phytochlorin-based sonosensitizers has significantly improved SDT, yet the absence of specialized sonodynamic systems limits their clinical translation. Herein, a dedicated ultrasound system along with a detailed step-by-step sonodynamic process from in vitro to in vivo is developed to activate phytochlorin-based sonosensitizers.

Sovleplenib in patients with primary or secondary warm autoimmune haemolytic anaemia: results from phase 2 of a randomised, double-blind, placebo-controlled, phase 2/3 study.

Background: Spleen tyrosine kinase inhibitors are potential treatment options for warm autoimmune haemolytic anaemia. This study aimed to assess the preliminary efficacy and safety of sovleplenib-an oral spleen tyrosine kinase inhibitor-in patients with warm autoimmune haemolytic anaemia in China. Here we report on the phase 2 results.

Saccharomyces cerevisiae inactivation during water disinfection by underwater plasma bubbles: Preferential reactive species production and subcellular mechanisms.

The escalating challenges posed by water resource contamination, especially exacerbated by health concerns associated with microbial fungi threats, necessitate advanced disinfection technologies. Within this context, non-thermal plasma generated within bubble column reactors emerges as a promising antifungal strategy. The effects of direct plasma bubbles within different discharge modes and thus-produced plasma activated water (PAW) on the inactivation of Saccharomyces cerevisiae are investigated.

Adverse events of 20-22G second-generation endoscopic ultrasound-guided fine-needle biopsy needles for solid lesions in the upper gastrointestinal tract and adjacent organs: Systematic review and meta-analysis.

Objectives: Previous research has conducted meta-analyses on the diagnostic accuracy of endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB). However, studies on adverse events (AEs) have been limited and sporadic and have included a highly diverse group of patients (with upper and lower gastrointestinal tract issues) and needles of varying sizes (19-22-25G). The purpose of this systematic review and meta-analysis was to determine the incidence of AEs related to the utilization of 20-22G second-generation EUS-FNB needles subsequent to puncture of the upper gastrointestinal tract and adjacent organs.

Single-Cell Heterogeneity of EGFR Pathway Is Linked to Unique Signatures of Drug Response and Malignancy in Patient Derived Glioblastoma Stem Cells.

Purpose: In glioblastoma, the therapeutically intractable and resistant phenotypes can be derived from glioma stem cells, which often have different underlying mechanisms from non-stem glioma cells. Aberrant signaling across the EGFR-PTEN-AKT-mTOR pathways have been shown as common drivers of glioblastoma. Revealing the inter and intra-cellular heterogeneity within glioma stem cell populations in relations to signaling patterns through these pathways may be key to precision diagnostic and therapeutic targeting of these cells.

Unlocking dual-mode enzyme activities on bacterial surface: Directional recognition and swift capture of Alicyclobacillus acidoterrestris from fruit juices.

The acidophilic and heat-resistant characteristics of Alicyclobacillus acidoterrestris (A. acidoterrestris) pose significant challenges to fruit juice production. Traditional thermal removal methods are often ineffective against this resilient bacterium.

CD63-high macrophage-derived exosomal miR-6876-5p promotes hepatocellular carcinoma stemness via PTEN/Akt-mediated EMT pathway.

Objective: Accumulating evidence suggests that microRNAs derived from macrophage exosomes can regulate the stemness and progression of cancer. However, the interaction mechanisms between HCC cells and tumor-associated macrophages remain unclear.

Methods: Exosomes were extracted from control or CD63 overexpression macrophages and co-cultured with HCC cells.

In situ structural-functional synchronous dissection of dynamic neuromuscular system via an integrated multimodal wearable patch.

Neuromuscular abnormality is the leading cause of disability in adults. Understanding the complex interplay between muscle structure and function is crucial for effective treatment and rehabilitation. However, the substantial deformation of muscles during movement (up to 40%) poses challenges for accurate assessment.

Integral-Omics: Serial Extraction and Profiling of Metabolome, Lipidome, Genome, Transcriptome, Whole Proteome and Phosphoproteome Using Biopsy Tissue.

The integrative multiomics characterization of minute amounts of clinical tissue specimens has become increasingly important. Here, we present an approach called Integral-Omics, which enables sequential extraction of metabolites, lipids, genomic DNA, total RNA, proteins, and phosphopeptides from a single biopsy-level tissue specimen. We benchmarked this method with various samples, applied the workflow to perform multiomics profiling of tissues from six patients with colorectal cancer, and found that tumor tissues exhibited suppressed ferroptosis pathways at multiomics levels.

The Phenotype Changes of Astrocyte During Different Ischemia Conditions.

Objectives: Dementia is becoming a major health problem in the world, and chronic brain ischemia is an established important risk factor in predisposing this disease. Astrocytes, as one major part of the blood-brain barrier (BBB), are activated during chronic cerebral blood flow hypoperfusion. Reactive astrocytes have been classified into phenotype pro-inflammatory type A1 or neuroprotective type A2.

Immune-related thyroid dysfunction as a positive prognostic factor for patients with lung cancer in China: a real-world retrospective study.

Introduction: The relationship between immune-related thyroid dysfunction (irTD) and survival rates in cancer patients remains unclear. Furthermore, the impact of variations in immunotherapy line numbers and pathological types among lung cancer patients on this relationship has not been fully elucidated. This study aims to evaluate the potential of irTD as a prognostic marker for immunotherapy in Chinese patients with lung cancer.

Historical record of trace elements since MIS 2 in a sediment core of Laizhou Bay, China.

The concentration of trace elements in sediments is a critical element in the quality of nearshore environments. Geochemical background values are the normal concentrations of trace elements in the natural environment, and the use of different background values has resulted in different evaluations. Trace element (Cu, Pb, Zn, Cr, Cd, As, and Hg) concentration profiles along a sediment core were investigated to obtain background values and to assess the depositional processes and contamination levels in Laizhou Bay.

Synthesis of bioisosteres of caffeic acid phenethyl ester: 1,3,4-oxadiazole derivatives containing a catechol fragment with anti-inflammatory activities in vitro and in vivo.

Aimed to enhance the anti-inflammatory activity of caffeic acid phenethyl ester (CAPE), the oxadiazole derivatives were synthesized by substituting its ester group. The structure-activity relationships revealed that the electron-withdrawing group in the phenethyl moiety enhanced anti-inflammatory activity. The order of activity potency was F ≥ CF > Cl > NO > CN.

Development of dual polymerase spiral reaction for detection of Listeria monocytogenes and Staphylococcus aureus simultaneously.

Listeria monocytogenes and Staphylococcus aureus are prevalent foodborne pathogens responsible for poisoning humans with food. The present study was devoted to the establishment of a method based on dual polymerase spiral reaction (dual-PSR) and melting curve analysis for concurrent identification L. monocytogenes and S.

Improved ovalbumin accurate quantitative performance in processed foods by full-length isotope-labeled protein.

Ovalbumin (OVA) is a high-risk allergen with complex tertiary structure in food samples. Here, we developed an accurate UPLC-MS/MS-based assay to improve OVA quantitative performance in processed foods. Full-length isotope-labeled OVA proteins (OVA-I) were synthesized using stable isotope labeling by amino acids in cell culture (SILAC) technique and employed as functional internal standards to ensure similar cleavage sites between internal standards and analytes.

The application of ChatGPT in nursing: a bibliometric and visualized analysis.

Objective: With the development of ChatGPT, the number of studies within the nursing field has increased. The sophisticated language capabilities of ChatGPT, coupled with its exceptional precision, offer significant support within the nursing field, which includes clinical nursing, nursing education, and the clinical decision-making process. Preliminary findings suggest positive outcomes, underscoring its potential as a valuable resource for enhancing clinical care.

Identification of common diagnostic genes and molecular pathways in endometriosis and systemic lupus erythematosus by machine learning approach and in vitro experiment.

Growing research suggests that endometriosis and systemic lupus erythematosus (SLE) are both chronic inflammatory diseases and closely related, but no studies have explored their common molecular characteristics and underlying mechanisms. Based on GEO datasets, differentially expressed genes in the endometriosis cohort and the SLE cohort were screened using Limma and weighted gene co-expression network analysis (WGCNA), and prediction signatures were constructed using LASSO logistic regression analysis, respectively. Four co-diagnostic genes (PMP22, QSOX1, REV3L, SP110) were identified for endometriosis and SLE.

Oxygen adsorption and activation control the photochemical activity of common iron oxyhydroxide polymorphs in mediating oxytetracycline degradation under visible light.

The natural minerals with semiconducting properties possess photochemical activity through generating reactive oxygen species (ROSs) and affect the fate of adsorbed organic pollutants. Iron oxyhydroxides occur in different polymorphic structures under various geological and climatic conditions in natural environment. However, the difference in their photoactivity has not been well understood.

Corrigendum: Apolipoprotein A-I attenuates peritoneal fibrosis associated with peritoneal dialysis by inhibiting oxidative stress and inflammation.

[This corrects the article DOI: 10.3389/fphar.2023.

Knowledge, attitudes and practices among patients with impacted wisdom teeth towards teeth extraction in Jinan, Shandong Province, China: a cross-sectional study.

Objectives: This study aimed to assess the knowledge, attitudes and practices (KAP) of patients with impacted wisdom teeth towards tooth extraction, with the intention of identifying both gaps and opportunities for improved dental health education and practices.

Design: A cross-sectional study using a web-based questionnaire.

Setting: The study was conducted at the Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, and Jinan Stomatological Hospital.

Bioinformatics Identification of angiogenesis-related biomarkers and therapeutic targets in cerebral ischemia-reperfusion.

Promoting vascular endothelial cell regeneration can enhance recovery from cerebral ischemia reperfusion injury (CIRI), but there is a lack of bioinformatic studies on angiogenesis-related biomarkers in CIRI. In this study, we utilized the GSE97537 and GSE61616 datasets from GEO to identify 181 angiogenesis-related genes (ARGs) and analyzed differentially expressed genes (DEGs) between CIRI and control groups. We converted ARGs to 169 rat homologues and intersected them with DEGs to find DE-ARGs.

Toward Green Liquid Nitrogen Fertilizer Synthesis: Plasma-Driven Nitrogen Oxidation and Partial Electrocatalytic Reduction.

Liquid fertilizers, particularly when integrated with precision irrigation systems, offer a more efficient and sustainable alternative to traditional solid nitrogen fertilizers. The industrial production of ammonium nitrate (NHNO) is environmentally detrimental due to its reliance on fossil fuels. This study introduces an innovative air-to-NOx-to-NHNO pathway for synthesizing liquid nitrogen fertilizer.

Current Progress on Postoperative Cognitive Dysfunction: An Update.

Postoperative cognitive dysfunction (POCD) represents a significant clinical concern, particularly among elderly surgical patients. It is characterized by a decline in cognitive performance, affecting memory, attention, coordination, orientation, verbal fluency, and executive function. This decline in cognitive abilities leads to longer hospital stays and increased mortality.

Ratiometric sensor based on Ag-mediated luminescence of Tb-DNA complexes for visual detection of 4-aminophenol.

Development of accurate, convenient and portable methods for monitoring 4-aminophenol (4-AP) is extremely important because of its strong toxicity. Here, a ratiometric fluorescence sensor based on Ag-enhanced luminescence of Tb-DNA complexes has been presented for the detection of 4-AP. The luminescence of Tb-DNA complexes is enhanced about 30 times by Ag, which can trigger energy transfer from DNA to Tb more efficiently.