RNA-seq reveals temporal differences in the transcriptome response to acute heat stress in the Atlantic salmon (Salmo salar).

Acute heat stress is common in aquaculture and can affect diverse physiological processes in fish; however, different species of fish have various mechanisms for heat stress adaptation. In this study, we profiled the transcriptome responses of the Atlantic salmon (Salmo salar) to heat stress at 23 °C for 6 or 24 h, compared with that of fish at a normal temperature of 13 °C. The liver was selected as the target tissue for this analysis.

iTRAQ-based quantitative proteomic analysis of embryonic developmental stages in Amur sturgeon, Acipenser schrenckii.

The Amur sturgeon, Acipenser schrenckii, is an important aquaculture species in China with annual production of about 150 thousand tons in 2015. In this study, we investigated the regulatory proteins and pathways affecting embryonic development of Amur sturgeon, by analyzing of the differential proteomes among four embryonic developmental stages using isobaric tags for relative and absolute quantitation (iTRAQ), combined with the analysis of effects of microelements and antioxidants on embryonic development. Seventy-four, 77, and 76 proteins were differentially expressed according to iTRAQ analysis between the fertilized egg and blastula, blastula and neurula, and neurula and heart-beat stages, respectively.

Identification of genes in the hypothalamus-pituitary-gonad axis in the brain of Amur sturgeons (Acipenser schrenckii) by comparative transcriptome analysis in relation to kisspeptin treatment.

Kisspeptin plays an important role in the reproduction and onset of puberty in vertebrates through stimulation of gonadotropin-releasing hormone (GnRH). However, the mechanisms whereby kisspeptin-related genes regulate sexual differentiation in teleosts are poorly understood. We aimed to study the relationship between the hypothalamus-pituitary-gonad (HPG) axis and sexual differentiation in relation to kisspeptin in the sturgeon Acipenser schrenckii.

Complete mitochondrial genome of Acipenser schrenckii (Acipenseriformes, Acipenseridae).

Abstract The complete nucleotide sequence of Acipenser schrenckii mtDNA is 16,592 bp long (Accession No. KC820796). The total mitochondrial genome is consisted of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region.

[Analysis of organochlorine pesticides and pyrethroid pesticides in vegetables by gas chromatography-electron capture detection coupled with solid-phase extraction using multiwalled carbon nanotubes as adsorbent].

A multi-residue analytical method based on solid-phase extraction (SPE) with multiwalled carbon nanotubes (MWCNTs) as adsorbent was developed. The determination of 6 organochlorine pesticides and 7 pyrethroid pesticides in vegetables (including cucumber, cherry tomato, cabbage, lettuce, purple cabbage, leek, shallot and onion) was carried out by gas chromatography-electron capture detection (GC-ECD). The GC-ECD method used two columns (HP-50 and HP-1) and two ECD detectors.

[The effect of hyperbaric oxygen therapy to different degree of hearing loss and types of threshold curve in sudden deafness patients].

Objective: To investigate the effect of hyperbaric oxygen therapy to different degrees of hearing loss and different types of threshold curve in sudden deafness patients, and establish a personalized therapy, so as to promote clinical effect to sudden deafness.

Method: One hundred and twenty cases were divided into two groups respectively (each group 60 cases). Drug treatment had been used in group A for two weeks.