Effect of heat stress and diet composition on performance of White Leghorn hens.

A total of 192 Single Comb White Leghorn pullets were divided into three groups to study the effect of heat stress and diet composition on feed intake and laying performance at 21, 25, and 33 weeks of age. For each age group all birds were fed a control diet [17.1% crude protein (CP), 2770 kcal metabolizable energy (ME)/kg] for a 7-day period.

Experimental transmission of duck hepatitis B virus.

Susceptibility to experimental infection with duck hepatitis B virus (DHBV) was explored, with the objective of defining procedures that were both rapid and reproducible. For the purpose of these experiments, a small flock of DHBV-free breeders was established as a source of susceptible eggs and ducklings, since ca. 10% of the ducks (all ages) from commercial flocks were DHBV infected.

Characterization of the genome of the agent of erythrocyte aplasia permits its classification as a human parvovirus.

It has recently been established that infection with a virus is the most common cause of transient arrest of erythrocyte production in the bone marrow, leading to aplastic crisis, in persons suffering chronic haemolytic anaemias. The physical characteristics of this human virus have suggested that it may be a member of the Parvoviridae. We report here that extraction of nucleic acid from this virus under annealing conditions yielded a single species of double-stranded DNA 5.

The multidisciplinary team approach to exenteration of the pelvis.

The experience of a newly formed, multidisciplinary, pelvic exenteration team is presented which consists of a gynecologic oncologist, a general surgeon and a urologist. The first 20 exenterations performed by this team are reviewed. Nine patients had no postoperative problems, and of the 11 patients who did have complications, all but two, which resulted in death, were corrected without resultant debilitation.

Identical genetic profiles in primary and metastatic bladder tumors.

Transitional cell carcinoma of the bladder has been shown repeatedly to possess abnormal genetic profiles. During long periods recurrent tumors in the bladder consistently show the same type of genetic profile with each recurrence. We report on a patient with metastatic bladder cancer who had similar genetic profiles of the primary lesion and thoracocentesis fluid from the metastatic site.

Prognosis in carcinoma of the urinary bladder based upon tissue blood group abh and Thomsen-Friedenreich antigen status and karyotype of the initial tumor.

Several markers of initial bladder carcinomas described recently may be clinically significant predictors of biological behavior of future recurrences. Comparison of the marker systems and assessment of the value of using multiple markers have been difficult, because the various markers have been studied in different patients. In this study, we compared four markers [chromosome mode, marker chromosomes, and expression of the ABH "blood group" antigens and the Thomsen-Friedenreich antigen (using immunoperoxidase or lectin immunoperoxidase methods)] in 39 patients presenting initially with low-stage bladder carcinoma and followed 3 to 11 years or until deep muscle invasion occurred.

Consequence of increased feed allowance for growing broiler breeder pullets as a means of stimulating early maturity.

Two hundred and forty Hubbard X Hubbard breeder pullets, 3 weeks of age, received conventional quantities of feed according to the breeder's recommendation or 5, 10, or 20% extra feed allowance. On reaching an average weight of 2.1 kg, birds were moved to laying cages, subjected to 14 hr of light per day, and offered a breeder diet at the breeder's recommended allowance.

Protein covalently bound to minus-strand DNA intermediates of duck hepatitis B virus.

Analysis of duck hepatitis B viral DNA by gel electrophoresis, Southern blotting, and binding to benzoylated naphthoylated DEAE-cellulose showed that a protein is bound to the minus-strand virion DNA as well as to the full-length single strand, minus-strand species, and minus-strand DNA intermediates isolated from replicating complexes present in infected duck liver. By utilizing a modified dideoxynucleotidyl sequencing method, it was shown that the protein is covalently bound to the smallest detectable growing strands (ca. 30 bases) and that minus-strand synthesis begins at a unique site.

Novel forms of woodchuck hepatitis virus DNA isolated from chronically infected woodchuck liver nuclei.

We cloned several unique forms of woodchuck hepatitis virus, a DNA virus closely related to hepatitis B virus, from a chronically infected woodchuck liver. Each of the three clones contained more than two genome equivalents of viral sequences with extensive rearrangements and no detectable cellular sequences. From the frequency by which they were isolated from a library of recombinant clones, we estimate that they are present in approximately one copy per cell.

Effect of dietary protein and energy on broiler performance and production costs.

Varying levels of dietary protein (16, 20, 24, 28, 32, 36%) and energy (2600, 2800, 3000, 3200, 3400, 3600 kcal [metabolizable energy] ME/kg) were fed in a factorial arrangement to broilers to determine the effect of alteration in nutrient density on performance and economic traits. Body weight and feed efficiency improved with increased dietary protein or energy. However, a significant interaction between protein and energy indicated the importance of a balanced energy: protein ratio to achieve optimum performance.

Effect of dietary protein and energy on broiler carcass composition and efficiency of nutrient utilization.

Day-old broilers were offered diets varying in dietary protein (16, 20, 24, 28, 32, 36%) and energy (2600, 2800, 3000, 3200, 3400, 3600 kcal [metabolizable energy] ME/kg) to 49 days of age to determine the effect of alteration in nutrient density on carcass composition and efficiency of nutrient utilization. Abdominal and total carcass fat contents responded similarly to alterations in dietary protein and energy, whether expressed on a percentage or absolute weight basis. However, maximum protein deposition occurred with the 20% crude protein diet, in contrast to the increase in percent carcass protein observed above this level.

Use of single-stage low protein diets for growing Leghorn pullets.

Two experiments were conducted in which growing White Leghorn pullets were fed single-stage 14% CP diets from 0 to 19 weeks. In the first trial, diet treatments were 1) conventional step-down protein, 2) reverse-protein, 3) 14% CP single-stage with methionine and lysine adjusted to 2 and 5% of CP, respectively, 4) as per 3 to 12 weeks followed by 14% CP with no amino acid constraints to 19 weeks. Each treatment was tested with 14 replicated cages each of 11 commercial strain Leghorn pullets.

Asymmetric replication of duck hepatitis B virus DNA in liver cells: Free minus-strand DNA.

In order to study the replication of the DNA genome of duck hepatitis B virus, an avian virus related to human hepatitis B virus, we have characterized viral DNAs present in the livers of viremic ducks by agarose gel electrophoresis and the Southern blot procedure. In addition to relaxed circular DNA similar to virion DNA, livers contained a heterogeneous population of rapidly migrating species. The conformation of the rapidly migrating species was markedly sensitive to salt, suggesting that these species were largely single stranded.

Blood group precursor T-antigen expression in human urinary bladder carcinoma.

Tumor deletion of the ABH blood group antigens (BGAg) heralds an unfavorable prognosis in human bladder cancer. The T antigen (Thomsen-Friedenreich antigen: TAg), a precursor of other BGAg, has previously been found in malignant but not most normal cells, in which the TAg is cryptic but can be unmasked with neuraminidase (NMD). We investigated the prognostic significance of TAg expression in bladder cancer by staining paraffin sections with a T-specific lectin (peanut agglutinin [PNA]) immunoperoxidase technic.

Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from hepatocellular carcinomas of woodchucks.

Woodchuck hepatitis virus (WHV), like the related hepatitis B virus, induces in its natural host hepatocellular carcinomas that contain integrated viral sequences. As a first step in determining whether and how the integrated sequences contribute to formation of the tumors in which they are found, we have cloned two such integrations of WHV and have determined their structure by restriction mapping and heteroduplex electron microscopy. The identity of the cloned sequences was confirmed by comparison of restriction sites in the clones with those located by Southern blot analysis of tumor DNA.

Replication of the genome of a hepatitis B--like virus by reverse transcription of an RNA intermediate.

Duck hepatitis B virus, a DNA virus closely related to human hepatitis B virus, was studied in infected duck liver. Subviral particles resembling the viral nucleocapsid cores were isolated from persistently infected liver and shown to have a DNA polymerase activity that utilizes an endogenous template and synthesizes both plus- and minus-strand viral DNA. Synthesis of the viral minus-strand DNA utilized an RNA template that was degraded as it was copied.

Monte Carlo description of time- and space-resolved multiple forward scatter in natural water.

The universal scatter function for natural water produces beam spread patterns in multiple forward scatter which remain compact in time and angular subtense, even neglecting absorption.

Performance of laying hens subjected to intermittent lighting initiated at 24 weeks of age.

Leghorn pullets of a commercial strain were subjected to a conventional light program of constant photoperiod or an intermittent programming involving, during the house of conventional "lights-on", 45 min light (L): 15 min dark (D) from 24 to 27 weeks of age; 30 min L:30 min D from 28 to 35 weeks; and 14 min L:45 min D from 36 weeks to the duration of the trial. Each light treatment was tested with 12 replicate groups of 14 individually caged birds housed in adjacent rooms, with all birds fed a standard 15% crude protein, 2750 kcal ME/kg diet. Intermittent lighting resulted in a significant (P less than .