A facile dual-mode aptasensor based on AuNPs@MIL-101 nanohybrids for ultrasensitive fluorescence and surface-enhanced Raman spectroscopy detection of tetrodotoxin.

The development of ultrasensitive, reliable, and facile detection technologies for trace tetrodotoxin (TTX) is challenging. We presented a facile dual-mode aptamer-based biosensor (aptasensor) for ultrasensitive fluorescence and surface-enhanced Raman spectroscopy (SERS) detection of TTX by using gold nanoparticles (AuNPs)-embedded metal-organic framework (MOF) nanohybrids (AuNPs@MIL-101) because of their superior properties. A TTX-specific aptamer labelled with fluorescence and Raman reporter cyanine-3 (Cy3-aptamer) was selected as the recognition element and signal probe.