Effects of cell entrapment on nucleic acid content and microbial diversity of mixed cultures in biological wastewater treatment.

The effects of entrapment on nucleic acid content and microbial diversity of mixed cultures in biological municipal wastewater treatment were investigated. Deoxyribonucleic acid content increased 1.6-5.

A new method to determine initial viability of entrapped cells using fluorescent nucleic acid staining.

Entrapped bacterial cells are widely used in several biotechnological applications. Cell entrapment procedures are known to affect the viability of bacterial cells. To determine the effect of entrapment procedures on viability of bacterial cells, dissolution of the entrapment matrices using chelating agents or heat is required immediately after the entrapment is completed.

Nitrate removal from agricultural infiltrate by bioaugmented free and alginate entrapped cells.

A bench-scale sand column experiment was conducted to investigate nitrate removal from synthetic agricultural infiltrate by denitrifying bacterial cells entrapped in calcium alginate compared to free cells. The effects of methanol as a carbon source and cell loading were examined. Low (0 to 50%) nitrate removal was observed in both entrapped and free-cell columns without methanol supplement.

Mineralization and biodegradability enhancement of natural organic matter by ozone-VUV in comparison with ozone, VUV, ozone-UV, and UV: effects of pH and ozone dose.

The increase in mineralization and biodegradability of natural organic matter (NOM) by ozone-vacuum ultraviolet (VUV) in comparison with ozone, VUV, ozone-ultraviolet (UV), and UV were investigated. The effects of operating parameters including pH and ozone dose were evaluated. Results showed that the mineralization rate of dissolved organic carbon (DOC) provided by the processes tested was in the following order: ozone-VUV > VUV > ozone-UV > ozone > UV.

A feasibility study of immobilized and free mixed culture bioaugmentation for treating atrazine in infiltrate.

A feasibility study of phosphorylated-polyvinyl alcohol immobilized and free mixed bacterial culture bioaugmentation for removing atrazine in agricultural infiltrate was conducted utilizing a sand column setup. The effects of bacterial cell loading and infiltration rate on atrazine degradation were investigated by short-term tests in which the amount of synthetic infiltrate fed through was five times of the void volume (five pore volumes) of the sand column. In addition, the loss of the inoculated atrazine-degrading cultures and the change of bacterial community were determined.

Atrazine remediation in agricultural infiltrate by bioaugmented polyvinyl alcohol immobilized and free Agrobacterium radiobacter J14a.

Bench-scale sand column breakthrough experiments were conducted to examine atrazine remediation in agricultural infiltrate by Agrobacterium radiobacter J14a (J14a) immobilized in phosphorylated-polyvinyl alcohol compared to free J14a cells. The effects of cell loading and infiltration rate on atrazine degradation and the loss of J14a were investigated. Four sets of experiments, i) tracers, ii) immobilized dead cells, iii) immobilized cells, and iv) free cells, were performed.

Atrazine removal in agricultural infiltrate by bioaugmented polyvinyl alcohol immobilized and free Agrobacterium radiobacter J14a: a sand column study.

Bench-scale sand column breakthrough experiments were conducted to examine atrazine removal in agricultural infiltrate by Agrobacterium radiobacter J14a (J14a) immobilized in phosphorylated-polyvinyl alcohol compared to free J14a cells. The effects of cell loading and infiltration rate on atrazine degradation and the loss of J14a were investigated. Four sets of experiments, (i) tracers, (ii) immobilized dead cells, (iii) immobilized cells, and (iv) free cells, were performed.