Bioengineering of glucan coated silver nanoparticles as dynamic biomedical compound; in vitro and in vivo studies.

The use of silver nanoparticles (AgNPs) gaining importance for the treatment of microbial infections and are in great demand due to their efficient broad antibacterial action but there is only one problem that silver nanoparticles can cause tissue damage. Therefore, the present study evaluated antimicrobial potential and intricacy of glucan coated silver nanoparticles in comparison with free silver nanoparticles. In this study, glucan coated silver nanoparticles (Glucan-AgNPs) by using Pleurotus spps.

Phytochemical profiling and anticancer potential of extracts against arsenic trioxide induced liver fibrosis in rat model.

Introduction: Arsenic trioxide (AsO) is an environmental contaminant that may cause hepatic injuries. AsO-induced liver injuries are detected as an underlying cause of hepatocellular carcinoma (HCC) around the globe. The present study aimed to investigate the potential of (GL) extracts against oxidative stress and apoptotic activity in HCC-induced rats and to explore molecular docking analysis of phytocompounds of .

Emerging trends and future challenges of advanced 2D nanomaterials for combating bacterial resistance.

The number of multi-drug-resistant bacteria has increased over the last few decades, which has caused a detrimental impact on public health worldwide. In resolving antibiotic resistance development among different bacterial communities, new antimicrobial agents and nanoparticle-based strategies need to be designed foreseeing the slow discovery of new functioning antibiotics. Advanced research studies have revealed the significant disinfection potential of two-dimensional nanomaterials (2D NMs) to be severed as effective antibacterial agents due to their unique physicochemical properties.

Synthesis, characterization and anti-breast cancer potential of an incensole acetate nanoemulsion from essential oil; , , and study.

The characteristics of phytocompounds and essential oils have undergone extensive research in the medical and pharmaceutical sectors due to their extensive usage. In spite of the fact that these molecules are widely used, terpenes, terpenoids, and their derivatives have not yet been well characterized. This study intends to evaluate the prospective activity of incensole acetate (IA), a compound identified and isolated from essential oil by GC/MS analysis and column chromatography, and to analyze the anticancer effect of an IA biosynthesized nanoemulsion against breast cancer.

Determination of Nutritional and Biochemical Composition of Selected spps.

The global demand for good quality food is going to be increased gradually. Mushrooms are broadly used as healthy nutritious meals. The nutritional values of extracts from four distinct species-, , , and -were determined in the current study.

Assessment of Hepatic Profile in Acquired Aplastic Anemia: An Experience From Pakistan.

Introduction:  Aplastic anemia (AA) is characterized by pancytopenia and hypocellular marrow in the absence of an abnormal infiltrate or increase in reticulin fibrosis. The diagnosis of AA is challenging at times due to decreased cellularity and overlapping morphological features with other bone marrow failure syndromes. Hepatitis-associated aplastic anemia (HAAA) is a rare variant in which patients typically present with jaundice and hepatitis followed by pancytopenia almost within 6 months.

Coronavirus Genomes and Unique Mutations in Structural and Non-Structural Proteins in Pakistani SARS-CoV-2 Delta Variants during the Fourth Wave of the Pandemic.

Genomic epidemiology of SARS-CoV-2 is imperative to explore the transmission, evolution, and also pathogenicity of viruses. The emergence of SARS-CoV-2 variants of concern posed a severe threat to the global public health efforts. To assess the potential consequence of these emerging variants on public health, continuous molecular epidemiology is of vital importance.

Identification of Peptides as Novel Inhibitors to Target IFN-, IL-3, and TNF- in Systemic Lupus Erythematosus.

Autoimmune disorder is a chronic immune imbalance which is developed through a series of pathways. The defect in B cells, T cells, and lack of self-tolerance has been greatly associated with the onset of many types of autoimmune complications including rheumatoid arthritis, systemic lupus erythematosus (SLE), multiple sclerosis, and chronic inflammatory demyelinating polyneuropathy. The SLE is an autoimmune disease with a common type of lupus that causes tissue and organ damage due to the wide spread of inflammation.

Biochemical Stress Markers, Antioxidants, and Infectious Wound-Healing Potential of UV Irradiation and Salt Stress Effects on the Pre-Treated Seed of Bitter Melon ( L.).

Purpose: The secondary metabolites in plants are the basis of defense and stress balance, which is an important aspect in plant growth. The UV-B treatment (a biotic stress) and salt stress on bitter melon ( L.) were studied, and the impact of pre-sowing seed treatment was evaluated on the basis of biochemical and enzymatic biomarkers, antioxidants, and wound-healing potential during early growth stages.

Association of Viruses in the Development of Cardiovascular Diseases.

Cardiovascular diseases (CVD), primarily inflammatory cardiomyopathy, are characterized by the infiltration of inflammatory cells into the myocardium. It has a relatively high risk of deteriorating heart function and has heterogeneous etiologies. Inflammatory cardiomyopathy is mainly mediated by viral infections but can also be mediated by protozoa, fungal or bacterial infections.

The emerging role and significance of circular RNAs in viral infections and antiviral immune responses: possible implication as theranostic agents.

Circular RNAs (circRNAs) are ubiquitously expressed, covalently closed rings, produced by pre-mRNA splicing in a reversed order during post-transcriptional processing. Circularity endows 3'-5'-linked circRNAs with stability and resistance to exonucleolytic degradation which raises the question whether circRNAs may be relevant as potential therapeutic targets or agents. High stability in biological systems is the most remarkable property and a major criterion for why circRNAs could be exploited for a range of RNA-centred medical applications.

Simultaneous fluorometric determination of the DNAs of Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus by using an ultrathin metal-organic framework (type Cu-TCPP).

Ultrathin (<10 nm) nanosheets of a metal-organic framework (MOF-NSs) were prepared in high-yield and scalable production by a surfactant-assisted one-step method. The MOF-NSs possess distinguished affinity for ssDNA but not for dsDNA. This causes the fluorescence of the labeled DNA to be quenched.

Anticancer, antithrombotic, antityrosinase, and anti-α-glucosidase activities of selected wild and commercial mushrooms from Pakistan.

Mushrooms have been accepted as nutraceutical foods because of their high nutritional and functional values. They have also gained interest due to their medicinal properties, economic importance, and organoleptic merit. In this study, wild and four commercial mushrooms, that is, and from Pakistan were screened for their biological activities such as anticancer, antityrosinase, anti-α-glucosidase, and antithrombotic activities from their methanol, ethanol, and water extracts.

Solution-Phase Synthesis of Platinum Nanoparticle-Decorated Metal-Organic Framework Hybrid Nanomaterials as Biomimetic Nanoenzymes for Biosensing Applications.

The synthesis of nanomaterials with specific properties and functions as biomimetic nanoenzymes has attracted extensive attention in the past decades due to their great potential to substitute natural enzymes. Herein, a facile and simple method for the preparation of platinum nanoparticle (PtNP)-decorated two-dimensional metal-organic framework (MOF) nanocomposites was developed. A ligand with heme-like structure, Fe(III) tetra(4-carboxyphenyl)porphine chloride (TCPP(Fe)), was applied to synthesize MOF nanosheets (denoted as Cu-TCPP(Fe) nanosheets) in high yield.

Structural and functional annotation of citrate synthase from Aspergillus niger ANJ-120.

Citrate synthase (CS) is involved in citric acid biosynthesis which is a well-established metabolic pathway. The condensation of acetyl-CoA with oxaloacetate is catalyzed by CS. Citric acid (CA) has a number of applications in pharmaceutical industry.

Wild Mushrooms: A Potential Source of Nutritional and Antioxidant Attributes with Acceptable Toxicity.

This paper describes in detail proximate composition, nutritional profile, phytochemical constituents, antioxidant activities, antimicrobial potential, and antihemolytic activity (towards human erythrocytes) of various fractions of wild . Proximate analysis established that wild comprises about 87.02±5.

Clinicohematological and cytogenetic profile of myelodysplastic syndromes in Pakistan-compare and contrast.

Background: Myelodysplastic syndromes (MDS) are clonal stem cell disorders exhibiting cytopenias, ineffective hematopoiesis and morphological dysplasia. Bone marrow cytogenetics, inspite of being incorporated as mandatory tool in diagnosis are done less frequently due to limited availability of this technique in Pakistan. The aim of the study was to study baseline clinicohematological and cytogenetic characteristics of patients presenting with de novo MDS.