Plant bioactive compounds driven microRNAs (miRNAs): A potential source and novel strategy targeting gene and cancer therapeutics.

Irrespective of medical technology improvements, cancer ranks among the leading causes of mortality worldwide. Although numerous cures and treatments exist, creating alternative cancer therapies with fewer adverse side effects is vital. Since ancient times, plant bioactive compounds have already been used as a remedy to heal cancer.

Crosstalk between protein misfolding and endoplasmic reticulum stress during ageing and their role in age-related disorders.

Maintaining the proteome is crucial to retaining cell functionality and response to multiple intrinsic and extrinsic stressors. Protein misfolding increased the endoplasmic reticulum (ER) stress and activated the adaptive unfolded protein response (UPR) to restore cell homeostasis. Apoptosis occurs when ER stress is prolonged or the adaptive response fails.

Standardized leaf extract induces the apoptotic HeLa cells death via microRNA regulation: identification, validation, and therapeutic potential.

var. angustifolia Thw. (Annonaceae), is a famous traditional medicinal plant in Asia.

GC-MS-based profiling and ameliorative potential of Stapf ex Haines fruit against cardiac and testicular toxicity: An study.

Fruit of Stapf ex Haines ( is a feed additive and is commonly used against cardiac dysfunction, fever, asthma, diarrhea, gastrointestinal ailments, and skin diseases. In this study, we aimed to evaluate the metabolic profile and antioxidant potential of fruit against carbon tetrachloride (CCl)-induced cardiotoxicity and testicular toxicity in rats. Gas Chromatoghraphy-Mass Spectrometry (GC-MS) analysis of fruit for the identification of potential metabolic profile, followed by methanolic extract of and its derived fractions including n-hexane, ethyl acetate, chloroform, butanol, and aqueous were used to assess the antioxidant potential of fruits.

Methanolic Extract Ameliorates CCl-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats.

The main objective of this study was to investigate the hepatoprotective potency of the methanol extract against CCl-induced liver damage in rats. HPLC technique was used to estimate the presence of polyphenols in the methanol extract of (PCM), while proximate analysis revealed the presence of carbohydrates, lipids, and moisture in the extract. The antioxidant potential of PCM was evaluated by 2,2-diphenylpicrylhydrazyl (DPPH) and reducing power assay, which showed a high percentage of inhibition against free radicals.

Synergistic Antimicrobial Activity of Ceftriaxone and Methanol (MEPL) Leaf Extract against Methicillin-Resistant and Modulation of Gene Presence.

Medicinal plants are an essential source of traditional curatives for numerous skin diseases. (Sonn.) Thwaites (Annonaceae family) used to cure skin illnesses.

Green Carbon Dots: Synthesis, Characterization, Properties and Biomedical Applications.

Carbon dots (CDs) are a new category of crystalline, quasi-spherical fluorescence, "zero-dimensional" carbon nanomaterials with a spatial size between 1 nm to 10 nm and have gained widespread attention in recent years. Green CDs are carbon dots synthesised from renewable biomass such as agro-waste, plants or medicinal plants and other organic biomaterials. Plant-mediated synthesis of CDs is a green chemistry approach that connects nanotechnology with the green synthesis of CDs.

Efficacy of citric acid chelate and Bacillus sp. in amelioration of cadmium and chromium toxicity in wheat.

Heavy metals contamination in agricultural soil is a major issue having drastic effects on plants and human health. To solve this issue, we have formulated and tested a new approach of fusion of inorganic (citric acid chelate) and organic (Bacillus sp.) amelioration methods for heavy metals.

Evaluation of antioxidant profile of various solvent extracts of Carissa opaca leaves: an edible plant.

Background: Carissa opaca leaves were conventionally recommended by local hakims in Pakistan for curing various human diseases including renal, hepatic and jaundice. In this work we arranged to study the antioxidant status of various fractions of C. opaca leaves through nine multifaceted assay systems.

Evaluation of Rumex hastatus leaves against hepatic fibrosis: a rat model.

Background: Rumex hastatus leaves have been widely used as food additive and for the treatment of various liver ailments. According to our previous studies, ethyle acetate (ERL) and methanolic (MRL) fractions of R. hastatus leaves are an accessible source of natural antioxidants.

Effect of Launaea procumbens extract on oxidative marker, p53, and CYP 2E1: a randomized control study.

Background: Ethyl acetate extracts of Launaea procumbens is used for the treatment of liver dysfunction as an herbal medicine in Pakistan. In this study, the protective effects of ethyl acetate extracts were evaluated against CCl4-induced liver injuries in rat.

Methods: To examine the protective effects against oxidative stress of carbon tetrachloride in rats, 30 male rats were equally divided into 5 groups (6 rats).

Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid peroxidation and trauma in rat.

Background: Carbon tetrachloride (CCl4) is a potent nephrotoxin, as it causes acute as well as chronic toxicity in kidneys. Therefore, this study was carried out to assess the pharmacological potential of different fractions of Carissa opaca fruits on CCl4-induced oxidative trauma in the kidney.

Methods: The parameters studied in this respect were the kidney function tests viz, serum profile, urine profile, genotoxicity, characteristic morphological findings, and antioxidant enzymatic level of kidneys.

CCl4 induced genotoxicity and DNA oxidative damages in rats: hepatoprotective effect of Sonchus arvensis.

Background: Sonchus arvesis is traditionally reported in various human ailments including hepatotoxicity in Pakistan. Presently we designed to assess the protective effects of methanolic extract of Sonchus arvesis against carbon tetrachloride induced genotoxicity and DNA oxidative damages in hepatic tissues of experimental rats.

Methods: 36 male Sprague-Dawley rats were randomly divided into 6 groups to evaluate the hepatoprotective effects of Sonchus arvensis against CCl4 induced genotoxicity, DNA damages and antioxidant depletion.

Cardioprotective role of leaves extracts of Carissa opaca against CCl4 induced toxicity in rats.

Background: Carissa opaca are used traditionally in Pakistan for the treatment of various human ailments. Therefore, the study is arranged out to assess the cardio protective potential of different fractions of Carissa opaca leaves on CCl4-induced oxidative trauma in kidney.

Methods: The parameters studied in this respect were the cardiac function test (CK (U/l), CKMB (U/l), genotoxicity (% DNA fragmentation), characteristic morphological findings and antioxidant enzymatic level of cardiac tissue homogenate.

Comprehensive assessment of phenolics and antiradical potential of Rumex hastatus D. Don. roots.

Background: Roots of Rumex hastatus (Polygonaceae) are traditionally used for the treatment of various ailments including liver and lung diseases. In this study, various solvent extracts of R. hastatus roots, like methanolic, n-hexane, ethyl acetate, chloroform, butanol and aqueous fractions were assessed through their antioxidant properties in vitro and determination of phenolic contents.

Effects of Carissa opaca fruits extracts on oxidative pulmonary damages and fibrosis in rats.

Background: Carissa opaca is a Pakistani fruit, traditionally used in the treatment of various human ailments including asthma and pulmonary damage. The present study investigated the protective effects of Carissa opaca against CCl4-induced oxidative stress in rat lungs.

Methods: To assess the protective effects of Carissa opaca, 42 Sprague-Dawley male rats (170-180 g) were randomly divided into 7 groups.

Estimation of flavoniods, antimicrobial, antitumor and anticancer activity of Carissa opaca fruits.

Background: Carissa opaca Stapf ex Hanes fruits is traditionally used in the treatment of asthma, hepatitis and microbial infections. The present study was arranged to investigate the antimicrobial, cytotoxic and antitumor activity of various fractions of C. opaca extract and its bioactive metabolites responsible for that activity.

Antitumor characterization of various fractions of Launaea procumbens.

The antitumor activity of various six fractions of Launaea procumbens were studied using the potato disk bioassay technique. Three concentrations (10, 100, and 1000 ppm) of all these six fractions were used for characterization of antitumor activity. Among these fractions, methanolic fraction exhibited significant inhibition of crown gall tumors caused by Agrobacterium tumefaciens followed by butanolic fraction.

Effect of Carissa opaca leaves extract on lipid peroxidation, antioxidant activity and reproductive hormones in male rats.

Background: Carissa opaca leaves are traditionally used in the treatment of male dysfunction and hormonal disorder as well as in oxidative stress in Pakistan and Asia. The present study was designed to assess the protective effects of methanolic extract of Carissa opaca leaves (MLC) on carbon tetrachloride (CCl4)-induced reproductive stress in male rats and bioactive constituents responsible for the activity.

Methods: CCl4 was induced in 42 male rats for eight weeks and checked the protective efficacy of methanolic extract of Carissa opaca leaves at various hormonal imbalances, alteration of antioxidant enzymes, DNA fragmentation levels and lipid peroxidation caused testicular fibrosis in testis while High performance Liquid Chromatography (HPLC) was used for detection of bioactive components.

Ameliorating effect of various fractions of Rumex hastatus roots against hepato- and testicular toxicity caused by CCl4.

Effect of methanolic extract of Rumex hastatus roots (MRR) and its derived fractions, n-hexane (HRR), ethyl acetate (ERR), chloroform (CRR), butanol (BRR), and aqueous extract (ARR), was studied against carbon tetrachloride (CCl4) induced hepato and testicular toxicity in rats. Intraperitoneal dose of 20 percent CCl4 (0.5 ml/kg bw) was administered twice a week for eight weeks to a group of rats.

Protective effects of Sonchus asper (L.) against KBrO₃-induced oxidative stress in rat testis.

Sonchus asper is used traditionally in the treatment of kidney inflammation, hormonal imbalance and impotency. Sonchus asper methanolic extract (SAME) was investigated for its possible preventive effect against potassium bromate (KBrO₃) induced oxidative damages in male rats using biochemical, molecular and histopathological markers in this study. 5 groups, each group of 6 rats were taken kept under standard conditions.

Modulation of carbon tetrachloride-induced nephrotoxicity in rats by n-hexane extract of Sonchus asper.

Sonchus asper is traditionally used in the treatment of renal dysfunction. In the present study, protective effects of S. asper against carbon tetrachloride (CCl4)-induced nephrotoxicity of rats were determined.

Carbon tetrachloride-induced lipid peroxidation and hyperglycemia in rat: a novel study.

Launaea procumbens methanol extract (LPME) was evaluated against carbon tetrachloride (CCl4)-induced pancreatic oxidative damage and hyperglycemia in rats. Various doses of the extract were administered to rats after 48 h of CCl4 treatment (3 ml/kg bodyweight (bw); intraperitoneally, 20% CCl4/olive oil) twice a week for 4 weeks. Coadministration of various concentrations of LPME (100, 150 and 200 mg/kg) ameliorated the toxicity of CCl4 and reversed the serum level of enzymes (amylase and lipase), glucose and hormone (insulin).

Protective effects of Sonchus asper against KBrO3 induced lipid peroxidation in rats.

Background: Sonchus asper is traditionally used in Pakistan for the treatment of reproductive dysfunction and oxidative stress. The present investigation was aimed to evaluate chloroform extract of Sonchus asper (SACE) against potassium bromate-induced reproductive stress in male rats.

Methods: 20 mg/kg body weight (b.