[The individual glycolysis enzymes in the enterocytes of the rat small intestine studied during exposure to ionizing radiation].

Activity and isoenzyme composition of certain enzymes of glycolysis of rat small intestine enterocytes was studied in the course of exposure to X radiation. Hexokinase activity was shown to increase significantly throughout the entire period of observation. Activity of pyruvate kinase and lactate dehydrogenase was inhibited at early (days 1-3) and increased at later (days 5-10) times of observation.

[A method of fractionating bone marrow cells in a density gradient using a mixture of Ficoll and Verografin].

The authors suggest a method for hemopoietic tissue cell fractionation into populations enriched for various hemopoietic elements. Centrifugation in a three-layer density gradient of mixtures of ficoll and verografin (d1 = 1.03 g/cm3, d2 = 1.

[The activity of carbohydrate metabolic enzymes in bone marrow cells of rats with radiation injuries].

The influence of ionizing radiation (154.8 mC/kg) on activity of some carbohydrate metabolism dehydrogenases in cells of the whole and fractionated rat bone marrow has been investigated. Different glucose metabolism units differently responded to radiation, the highest radiation response being exhibited by pentosophosphate cycle processes.

[The effect of hypoxic hypoxia on the activity of glycolysis enzymes in rat erythrocytes].

The oxygen-binding properties of hemoglobin, concentration of 2,3-diphosphoglycerate, activity of carbohydrate metabolism enzymes and kinetics of rat erythrocyte hemolysis have been studied at high altitudes. The hemoglobin affinity to oxygen, glycolysis enzyme activity and erythrocyte membrane resistance are established to increase at the initial period of adaptation. The activation of the pentose-phosphate pathway of the glucose, transformation and inhibition of the glycolytic process in these cells are observed on the 10th day.

[Amino acid sequence of Ondatra myoglobin (Ondatra zibethica). Characteristic features of myoglobins from semiaquatic animals].

Based on the amino acid composition of globin, amino acid analysis and N-terminal sequencing of peptides as well as a comparative analysis of the primary structure of beaver, coypu rat and otter myoglobins with the use of the fingerprinting technique, the amino acid sequence of the major component of ondatra myoglobin including 153 amino acid residues was reconstructed. The results of a comparative analysis of the primary structure of myoglobin and the peculiarities of the functional morphology of myoglobins from semi-aquatic animals and sperm whale and the role of amino acid substitutions in the spatial structure of ondatra myoglobin are discussed.

[Effect of low-level chronic x-rays on the hemolytic stability and population composition of peripheral blood erythrocytes].

A study was made of the haemolytic stability and population composition of peripheral blood erythrocytes of rats chronically exposed to X-radiation of 0.258 mC/kg/day during 30 days. Haemolytic stability of erythrocytes changed at cumulative doses of 2.

[Oxygen-transport function and carbohydrate metabolism in rat erythrocytes during hypoxic hypoxia and treatment with insulin].

Hemoglobin affinity to oxygen, enzyme activity and metabolite concentration of carbohydrate metabolism were determined in erythrocytes of rats which were administered insulin solution. A valid decrease of the hemoglobin value P50 (pressure of hemoglobin half-saturation with oxygen), as well as a decrease of the enzyme activity of 2,3-diphosphoglycerate shunt and increase of the activity of regulatory glycolysis enzymes--hexokinase and pyruvate kinase in erythrocytes with multiple introduction of hormones to animals have been established. Such changes in rat erythrocytes were registered with the simultaneous effect of insulin and hypoxic hypoxia evoked by the "lift" of rats in the altitude chamber to the conditional altitude of 9000 m.

[Cationic blood proteins in suppurative and septic processes].

The level of cationic proteins was studied in 52 patients with peritonitis and purulent processes of soft tissues. The authors have shown its elevation to be dependent on the degree of the disease. The improved state of the patients and recovery was followed by a decreased content of cationic proteins in blood, their fractional composition being normalized.

[Comparative analysis of the peroxidase activity of myoglobins in mammals].

Studies have been made on the peroxidase activity of metmyoglobins in animals from various ecological groups--the horse Equus caballus, cattle Bos taurus, beaver Castor fiber, otter Lutra lutra, mink Mustela vison and dog Canis familiaris. It was found that the level of this activity in diving animals depends on the duration of their diving, whereas in terrestrial species--on the strength of muscular contraction.

[Primary structure of otter (Lutra lutra L.) myoglobin. II. Pepsin peptides of trypsin hydrolysate. Reconstruction of the polypeptide chain of the otter myoglobin globin component].

13 peptic peptides have been isolated from the insoluble (at pH 5.0) fraction of the tryptic hydrolysate of main chromatographic component of otter myoglobin and their amino acid composition and N-terminal amino acid sequences have been determined. The isolated peptides contain in total 40 amino acid residues.

[(ADP-ribose)n-transferase activity of enterocyte nuclei during radiation injury of small intestine mucosa in rats].

The (ADP-ribose)n transferase activity of enterocyte nuclei of small intestine is studied in rats in normal and under radiation injury of intestine mucosa. It is shown that kinetics of [14C]-NAD incorporation into the acid-insoluble fraction of enterocyte nuclei is of a two-step character when the medium contains 1-15 microM of NAD. The X-ray irradiation of animals (a dose of 0.

[Effect of thyroxine on the hemoglobin affinity to oxygen and 2,3-diphosphoglycerate level in rat erythrocytes].

Affinity of hemoglobin to oxygen was studied in rat erythrocytes under conditions of intraperitoneal administration of thyroxin in a dose of 0.4 mg per 100 g of animal weight each 24 h for five days. A significant right shift of the oxyhemoglobin dissociation curve is observed only on the 6th day simultaneously with the increase of the 2,3-diphosphoglycerate level in erythrocytes in the period under study.

[The effect of ionizing radiation on the functional properties of hemoglobin from fractionated cells of dog bone marrow].

Cell populations of dog bone marrow enriched by erythroid cells were obtained by fractionation in a sucrose density gradient. Functional properties of the haemoglobin isolated from nucleate erythrocyte precursors were studied during the radiation injury development. The haemoglobin of later nucleate forms of the erythroid series of irradiated dog bone marrow exhibited an increase in its affinity to oxygen.

[Primary structure of otter (Lutra lutra L.) myoglobin. 1. Soluble peptides of a tryptic hydrolysate].

The amino acid sequence of 24 soluble tryptic peptides has been determined for the main chromatographic component of otter myoglobin. Fractionation, identification and peptide isolation have been made by fingerprints. The isolated peptides contain in total 111 amino acid residues.

[Method of isolation of intact enterocytes from rat duodenal mucosa].

A procedure is suggested for obtaining intact enterocytes from rat duodenum mucosa by the tissue trypsinization in the isotonic Krebs solution (pH 7.9). The maximum yield of certain enterocytes is reached at the enzyme concentration of 0.

[Regulatory mechanisms of oxygen transport function of hemoglobin in irradiated animals].

The oxygen affinity of hemoglobin of both common and 2,3-diphosphoglycerate concentration-purified erythrocyte hemolysates, hemoglobin stability, 2,3-diphosphoglycerate concentration in erythrocytes, morphological blood content were studied in rabbits irradiated with X-rays in a dose of 0.155 C/kg. An increase in the oxygen affinity of common hemolysate hemoglobin at a higher 2,3-diphosphoglycerate concentration on the 1st, 4th day after irradiation and a decrease in the oxygen affinity of both common and purified hemolysate hemoglobin on the 18th-30th day after irradiation were observed.

[Structural anomalies of dog hemoglobin after ionizing irradiation].

Heterogeneity of dog hemoglobin is established with application of chromatographic analysis. Ionizing radiation (4 Grey) induces no changes in the ratio of hemoglobin components. The comparative dactylographical analysis of the hemoglobin components in norm and in different periods after irradiation revealed differences in responses to tryptophan in peptide T-25.

[Effect of diethylstilbestrol on conformation of the hemoglobin molecule].

The paper deals with the results obtained while measuring the surface presssure of hemoglobin monolayers containing diethylstilbestrol. It is shown that insignificant additions of diethylstilbestrol change the shape of the surface hemoglobin pressure isoterm. The addition of diethylstilbestrol to hemoglobin causes a considerable increase in the limiting value of the area corresponding to the substance mass unit in the monolayer.

[Heterogeneity of hemoglobins in hypoxia-resistant animals].

Beaver, otter and nutria hemoglobin chromatographical components were isolated, a comparative analysis of the studied proteins was carried out and their intraspecific heterogeneity was established. The chromatographical components of nutria hemoglobin proved to differ in their physical and chemical properties as well as in the structure.

[Comparative study of the myoglobin of the reindeer Rangifer tarandus, the elk Alces alces and the deer Cervus elaphus xanthopygus].

Myoglobin preparations from muscles of A. alces, R. tarandus and C.