evaluation of bioactivity of alginate/chitosan based osteoplastic nanocomposites loaded with inorganic nanoparticles.

The influence of two nanostructured osteoplastic materials with different compositions: i) alginate (Alg) matrix, loaded with Zn ions and nanostructured hydroxyapatite (HA) - S1/HA-Zn, and ii) chitosan (CS) matrix loaded with brushite nanoparticles (NPs, dicalcium phosphate dihydrate, DCPD) - S2/DCPD on the healing of an experimental femoral diaphysis defect was investigated. The structure of cellular elements and the lacunar tubular system of the regenerated bone tissue were studied by electron microscopy. Osteogenic cells on the surface and inside S1/HA-Zn formed bone tissue.

In Vivo feature of the regenerative potential of chitosan and alginate based osteoplastic composites doped with calcium phosphates, zinc ions, and vitamin D2.

Purpose: In vivo comparison of the regenerative potential of two calcium phosphate-biopolymer osteoplastic composites: а) based on alginate (Alg) and hydroxyapatite (HA) - Alg/HA/CS/Zn/D2, b) based on chitosan (CS) and brushite (DCPD) - CS/DCPD/D2.

Materials And Methods: 36 white male laboratory rats aged six months were used. A defect to the bone marrow canal in the middle of the femur diaphysis was made with a dental bur of 2 mm.

Metal ions doping effect on the physicochemical, antimicrobial, and wound healing profiles of alginate-based composite.

The alginate (Alg) matrix with immobilized hydroxyapatite (HAp) and zinc oxide (ZnO), cross-linked by chitosan (CS) and metal ions (Me) Ca, Zn, and Cu was created as a wound dressing. The effect of Me and their concentrations on water vapor transition, fluid handling, dehydration, drug release, and healing are shown. Me-containing samples have a lower sorption capacity, than a commercial Kaltostat, however, a much lower degree of their dehydration provides a longer wound wet.

Hydroxyapatite-biopolymers-ZnO composite with sustained Ceftriaxone release as a drainage system for treatment of purulent cavities.

Composite based on nano-sized hydroxyapatite (HA), zinc oxide (ZnO), chitosan (CS), alginate (Alg) with the function of sustained Ceftriaxone (CF) release was created as molecular sorption-aspiration drainage system (SADS), designed for the treatment of purulent cavities of various genesis, including peritonitis. ZnO stabilizes the composite structurally, reducing the swelling by 1.5 and porosity by 1.

Single-walled carbon nanotubes loaded hydroxyapatite-alginate beads with enhanced mechanical properties and sustained drug release ability.

Single-walled carbon nanotubes (SWCNTs) containing biomaterial with enhanced mechanical properties for the potential orthopedic application were synthesized and investigated. X-ray diffraction and X-ray fluorescence analysis were indications of the formation of calcium-deficient (Ca/P = 1.65) hydroxyapatite (HA) with a small carbonate content under influence of microwave irradiation.

Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.

The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride. The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P.

C fullerene loaded hydroxyapatite-chitosan beads as a promising system for prolonged drug release.

Bioactive composite material in the form of beads, based on natural polysaccharide Chitosan (CS), hydroxyapatite (HA), and C fullerene (C), was synthesized under influence of microwave irradiation (MW). The bead is a 3D matrix consisting of CS macromolecules cross-linked with sodium tripolyphosphate with HA and C particles immobilized in its structure. XRD and FTIR data confirmed the formation of calcium deficient carbonate substituted HA with needle-shaped nanocrystallites of about 80 nm.

C fullerene and its nanocomplexes with anticancer drugs modulate circulating phagocyte functions and dramatically increase ROS generation in transformed monocytes.

Background: C fullerene-based nanoformulations are proposed to have a direct toxic effect on tumor cells. Previous investigations demonstrated that C fullerene used alone or being conjugated with chemotherapeutic agents possesses a potent anticancer activity. The main aim of this study was to investigate the effect of C fullerene and its nanocomplexes with anticancer drugs on human phagocyte metabolic profile in vitro.

Composite material based on hydroxyapatite and multi-walled carbon nanotubes filled by iron: Preparation, properties and drug release ability.

The novel bioactive composite material based on hydroxyapatite and multi-walled carbon nanotubes filled by iron was synthesized by the "wet chemistry" method and characterized in detail by various experimental techniques including the X-ray diffraction, Fourier transform infrared and energy-dispersive X-ray fluorescence spectroscopy, thermogravimetric and differential thermal analysis. The swelling behaviour was quantified by measuring the changes in sample weight as a function of sample immersion time in a phosphate buffered saline (PBS). Bioactivity test was carried out by soaking the samples in PBS.

Structural features of hydroxyapatite and carbonated apatite formed under the influence of ultrasound and microwave radiation and their effect on the bioactivity of the nanomaterials.

The samples of hydroxyapatite and carbonate substituted hydroxyapatite (CHA) were obtained under the influence of physical factors, namely ultrasound (US) and microwave (MW) radiations. The results of Fourier transform infrared spectroscopy and X-ray diffraction analysis have proved the formation of the calcium deficient hydroxyapatite and B-type CHA with the Ca/P ratio in the ranges 1.62-1.

Controllability of brushite structural parameters using an applied magnetic field.

The paper studies the influence of low intensity static magnetic field on brushite structural and microstructural parameters using the X-ray diffraction and the transmission electron microscopy. This effect was shown to have various influences on DCPD (Dicalcium Phosphate Dihydrate) structure depending on a magnetic field configuration or time of synthesis, which allows achieving controllability of the main properties of an obtained material. The influence of the magnetic field leads mostly to the decrease of crystallite sizes with no impact on the crystal lattice parameters.

Formation of Solution-derived Hydroxyapatite Coatings on Titanium Alloy in the Presence of Magnetron-sputtered Alumina Bond Coats.

Hydroxyapatite Ca10(PO4)6(OH)2 (HAp) and calcium phosphate ceramic materials and coatings are widely used in medicine and dentistry because of their ability to enhance the tissue response to implant surfaces and promote bone ingrowth and osseoconduction processes. The deposition conditions have a great influence on the structure and biofunctionality of calcium phosphate coatings. Corrosion processes and poor adhesion to substrate material reduce the lifetime of implants with calcium phosphate coatings.

[Interaction of DNA nucleotide bases with anticancer drug ThioTEPA: molecular docking and quantum-mechanical analysis].

Using modern methods of molecular docking, quantum chemistry and quantum theory of atoms in molecules the interaction of anticancer drug ThioTEPA with isolated nucleotide bases and deoxyribonucleosidemonophosphates of DNA has been studied. Physical properties and some trends of binding have been established for the complexes of "nucleotide base + ThioTEPA" and "deoxyribonucleosidemonophosphate + ThioTEPA" types. It has been shown that strong hydrogen bonds of NH.

Silver-doped hydroxyapatite coatings formed on Ti-6Al-4V substrates and their characterization.

Coatings with antibacterial components for medical implants are recommended to reduce the risk of bacterial infections. Therefore hydroxyapatite (HA) coatings with addition of chitosan (CS) and silver (Ag) are proposed in this work in an attempt to resolve this problem. Ti-6Al-4V substrates were modified by a chitosan film to study the influence of surface modification on the formation of the HA-Ag and HA-CS-Ag coatings.

N-isopropylacrylamide-based fine-dispersed thermosensitive ferrogels obtained via in-situ technique.

Thermosensitive hydrogels with magnetic properties (ferrogels) are very promising for medical application, first of all, for the design of targeted delivery systems with controlled release of drugs and for magnetic hyperthermia and chemotherapy treatment of cancer. These magnetic hydrogels could be obtained using diverse techniques: ex- and in-situ syntheses. The present work is devoted to the study of magnetite (Fe(3)O(4)) formation inside the nanoreactors of (co)polymeric hydrogels.

A study of brushite crystallization from calcium-phosphate solution in the presence of magnesium under the action of a low magnetic field.

The paper discusses the crystallization of dicalcium phosphate dehydrate (DCPD) with subsequent transformation to nanocrystalline hydroxyapatite (НA) under the permanent magnetic field in the presence of magnesium. It was found that the presence of magnesium in the initial solution in concentrations of 0.01-0.

Characterization and in vivo evaluation of chitosan-hydroxyapatite bone scaffolds made by one step coprecipitation method.

Chitosan/hydroxyapatite scaffolds could be used for bone regeneration in case the application of auto- or allografts is impossible. The objective of the present work was to characterize and study in vivo biodegradation of simple chitosan/hydroxyapatite scaffolds. For this purpose, a series of chitosan/hydroxyapatite composites has been synthesized in aqueous medium from chitosan solution and soluble precursor salts by a one step coprecipitation method.

252Cf plasma desorption mass spectrometric study of interactions of steroid glycosides with amino acids.

252Cf Plasma desorption with time-of-flight mass spectrometry (TOF-PDMS) has been applied to comparative studies of the interactions of steroid glycosides (SGs) of the spirostan series with amino acids. SGs can interact with amino acids to form heteroclusters of the type [SG + aminoacid + H](+) and [SG + aminoacid + K](+). It is shown how the affinity of SGs for amino acids depends on the structures of both the SG carbohydrate chain and the SG aglycone, and on the nature of the amino acid side chain.

[Measurement of trace elements in blood serum by atomic absorption spectroscopy with electrothermal atomization].

Describes a method for measuring trace elements Cr, Mn, Co, Fe, Cu, Zn, and Mo in the blood serum using non-flame atomization (KAC 120.1 complex). Optimal conditions for preparing the samples were defined, temperature regimens for analysis of each element selected, and original software permitting automated assays created.

[Mass spectrometric and quantum chemical study of dimeric associates of nucleosides].

Deoxyribonucleosides H-bonded pairs were investigated using fast atom bombardment mass spectrometry and MNDO/H quantum chemistry method. It was shown that "rare" (enol or imin) forms of the nitrogen bases could form pairs with energy comparable with "canonical" base pair energy. It was shown that pair stability rows, which are measured using different experimental techniques, were in conformity each with other.

[Use of time-of-flight mass spectrometry with ionization division fragments of californium-252 for studying the mechanisms of action of drugs on DNA and its components].

Using soft-ionization mass spectrometry (252-Cf particle desorption mass spectrometry, PDMS) a minor adduct of anticancer drug prospidine and deoxyguanosine-5-phosphate (pdG) has been found. It has been shown experimentally that PDMS is very useful for study of biological mixtures as well as mechanisms of interactions between drugs and biomolecules.

Study of anticancer drug interaction with DNA by means of particle-induced desorption mass spectrometry: prospydine and deoxyguanosine-5'-monophosphate.

Using 252CF particle desorption mass spectrometry, the interaction between an antitumour drug prospydine (Pro) and deoxyguanosine-5'-monophosphate (pdG) has been studied. The adduct which corresponds to a peak at m/z 524.5 and which occurs as a result of the particular degradation of Pro during its alkylating of pdG has been found.