Publications by authors named "Sukhdeep Vohra"

Anaplasma marginale, a tick-borne obligate intracellular rickettsia is incriminated to cause heavy economic losses throughout the tropical and subtropical regions, including India. However, studies highlighting the phylogeography and demographic dynamics of A. marginale are very scant from India.

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  • Bovicola caprae, an ectoparasite affecting goats globally, was studied in India to confirm its molecular characteristics and analyze its genetic diversity.
  • The study identified two distinct lineages of B. caprae, with Indian sequences clustering with archived sequences from China and Iran.
  • Results showed low nucleotide diversity but high haplotype diversity, indicating recent population expansion, along with significant genetic differentiation and limited gene flow among populations.
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  • Hyalomma anatolicum is a tick species known for spreading diseases to both animals and humans, and this study focuses on its genetic structure using mitochondrial gene sequences.
  • Analysis of 75 tick sequences revealed 24 different haplotypes, with Haplotype 1 being the most common across various countries, indicating a recent increase in population size.
  • The study's findings suggest low genetic differentiation among populations and highlight the significance of H. anatolicum in disease transmission, reinforcing its status as an economically important species.
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Introduction: Equine theileriosis, an economically important disease that affects horses and other equids worldwide, is caused by a tick-borne intracellular apicomplexan protozoa . Genotyping of based on the 18S rRNA gene revealed the presence of two, three, four or five genotypes. In previous published reports, these genotypes have been labelled either alphabetically or numerically, and there is no uniformity in naming of these genotypes.

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  • - The study focused on analyzing the genetic diversity of *Theileria annulata* using the cytochrome b gene, examining samples from various countries, including India, to assess population structure and buparvaquone resistance.
  • - A maximum likelihood tree showed all analyzed *T. annulata* sequences grouped in one clade, but most haplotypes were unique to specific countries, indicating significant genetic differentiation with low gene flow between populations.
  • - While all *T. annulata* isolates remained sensitive to buparvaquone, two new mutations in the cyt b gene associated with drug resistance were uncovered for the first time, highlighting potential concerns for treatment efficacy.
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Purpose: Echinococcus granulosus sensu stricto (s. s.) consists of the most widespread genotypes (G1, G3) implicated in human cystic echinococcosis worldwide.

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Rhipicephalus microplus, a hematophagous vector prevalent in the tropics and subtropics, is responsible for huge economic losses throughout the globe. However, the taxonomy of the tick species, especially prevalent in north India and south China has been challenged in the recent past. The present study attempted to assess the cryptic status of R.

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  • Around 100 species of Physaloptera infect various animals, making identification, especially of larvae, challenging based on physical characteristics alone.
  • This study aimed to molecularly identify Physaloptera larval infections in northern palm squirrels, using the nuclear 18S rRNA gene for confirmation and phylogenetic analysis.
  • Histopathological examinations showed structural features of the parasites and highlighted the northern palm squirrel as a likely second intermediate host for P. praeputialis.
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Current chemotherapy against the Surra organism, Trypanosoma evansi has several limitations in terms of efficacy, toxicity, availability and emerging resistance. These reasons make the search of new chemo-preventive and chemo-therapeutic agent with high potency and low toxicity. Alkaloid phyto-molecules, berberine has shown promising anti-kinetoplastid activity against T.

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The present study was aimed at the identification, molecular characterization, and risk factor assessment of infection among sheep of Haryana province, north India. A total of 402 blood samples were collected from three different climatic zones of Haryana from March 2020 to September 2021. Light microscopy of blood smears revealed spp.

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  • The study investigated the genetic diversity of T. annulata using nearly complete 18S rRNA gene sequences from GenBank™, identifying 70 relevant sequences among 312 total entries.
  • A maximum likelihood phylogenetic analysis revealed two main clades; one included true T. annulata sequences while two isolates were closely related to T. lestoquardi.
  • The findings suggest a broader diversity of Theileria species in the region, including T. annulata and T. orientalis, highlighting potential misidentifications in previous studies.
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The present study had two aims: molecular characterization of Hyalomma dromedarii infesting one-humped camels of Haryana (North India), and assessment of the acaricidal potential of herbal methanolic extracts against H. dromedarii larvae in comparison to synthetic acaricides. Phylogenetics and population neutrality indices were assessed by targeting partial amplification of mitochondrial 16S rDNA sequences.

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  • Trypanosoma evansi is a protozoan parasite that causes a serious disease called Surra in horses, significantly impacting the equine industry globally, and currently treated with quinapyramine methyl sulfate (QPS).
  • The study aimed to understand how QPS affects the mRNA expression of 13 key genes in T. evansi, using in vitro cultures to measure changes after drug exposure.
  • Results showed that while some genes remained unchanged, several important genes were significantly up-regulated or down-regulated, suggesting that arginine kinase 1 and calcium ATPase I could be key to understanding QPS's anti-trypanosomal action.
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High cytotoxicity and increasing resistance reports of existing chemotherapeutic agents against T. evansi have raised the demand for novel, potent, and high therapeutic index molecules for the treatment of surra in animals. In this regard, repurposing approach of drug discovery has provided an opportunity to explore the therapeutic potential of existing drugs against new organism.

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  • * Genetic analysis using mitochondrial gene sequencing showed that Indian isolates are closely related to those from other countries, but one isolate from Himachal Pradesh formed a distinct subgroup.
  • * The research indicated low genetic diversity among metacestODES in North India, suggesting demographic expansion and a lack of genetic differentiation among the populations.
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Purpose: Theileriosis is an economically important tick-borne pathogen with a serious impact on livestock health and productivity. Despite the fact that bovine theileriosis has been widely investigated, there exists a paucity of information on these infections in small ruminants, especially in India. The present study was carried out to detect and differentiate different Theileria spp.

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Ovine theileriosis is an important tick-borne haemoprotozoan disease of sheep in tropical and subtropical regions, causing severe productivity and economic loss. There is a paucity of information related to molecular studies of ovine theileriosis from India. The present study identified different Theileria spp.

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  • - The study investigated the genetic diversity and haplotype network of *Theileria annulata* using 52 nearly complete 18S rRNA gene sequences from GenBank™, identifying 34 haplotypes with Haplotype 4 being the most widespread across countries like India, China, Turkey, and Iran.
  • - India had the highest number of haplotypes (25) while Turkey, China, Iran, and Italy had fewer; the analysis found no geographical clustering of haplotypes, indicating high gene flow among populations.
  • - The overall genetic analysis showed low nucleotide diversity but high haplotype diversity, suggesting recent population expansion and greater genetic variation within countries compared to between them.
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The present investigation was aimed to study the presence of Babesia caballi clades upon phylogenetic analysis of all available V4 hypervariable 18S rRNA gene sequences in GenBank in addition to the intra- and interclade genetic diversity in B. caballi and the distribution of parasite clades in different countries. Out of altogether 155 small-subunit ribosomal RNA gene sequences of B.

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Purpose: Hydatid disease is one of the neglected and challenging (for diagnosis as well as for treatment) parasitic diseases. Along with adverse effect on animal's health leading to production losses, hydatidosis is also associated with huge economic losses. The present study was envisaged with an aim to assess the phylogeny and pathological changes due to natural hydatid cysts in lungs and liver of slaughtered buffaloes in north India.

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