ALKBH5 regulates cardiomyocyte proliferation and heart regeneration by demethylating the mRNA of YTHDF1.

N-methyladenosine (mA) RNA modification, a dynamic and reversible process, is essential for tissue development and pathogenesis. However, the potential involvement of mA in the regulation of cardiomyocyte (CM) proliferation and cardiac regeneration remains unclear. In this study, we aimed to investigate the essential role of mA modification in heart regeneration during postnatal and adult injury.

Targeting LncDACH1 promotes cardiac repair and regeneration after myocardium infarction.

Neonatal mammalian heart maintains a transient regeneration capacity after birth, whereas this regeneration ability gradually loses in the postnatal heart. Thus, the reactivation of cardiomyocyte proliferation is emerging as a key strategy for inducing heart regeneration in adults. We have reported that a highly conserved long noncoding RNA (lncRNA) LncDACH1 was overexpressed in the failing hearts.

MicroRNA-92b-5p modulates melatonin-mediated osteogenic differentiation of bone marrow mesenchymal stem cells by targeting ICAM-1.

Osteoporosis is closely associated with the dysfunction of bone metabolism, which is caused by the imbalance between new bone formation and bone resorption. Osteogenic differentiation plays a vital role in maintaining the balance of bone microenvironment. The present study investigated whether melatonin participated in the osteogenic commitment of bone marrow mesenchymal stem cells (BMSCs) and further explored its underlying mechanisms.

[Identification of the apomixis in Poa pratensis L. using SDS-electrophoresis of endosperm reserve proteins].

To determine the characteristics of variability of seed reproduction in the Kentucky bluegrass (Poa pratensis), individual seed variability with respect to the composition of endospermal reserve proteins was studied. Comparative analysis of caryopses obtained by self-fertilization and free fertilization of plants I1 (no. 2-4) and I2 (no.

[The embryotoxic action of fragment C-I-6 of human interleukin-2].

The peptide C-I-6 is a modified fragment (sequence 59-72) of human interleukin-2 and stimulates regeneration of hepatic cells and tissues. Study of the possible embryotoxic and teratogenic properties of C-I-6 showed that in some cases it induces the following statistically significant changes: reduces the number of viable fetus, increases the occurrence of hydronephrosis and the number of external and internal hematomas, increases the weight and size of the embryo. It is concluded that intraperitoneal administration of C-I-6 causes a mild embryotoxic and teratogenic effect in rats.

[The use of preparations of protozoan origin as agents for the prevention and early therapy of combined radiation-thermal injuries].

Radioprotective and therapeutic effects of astazian and astazilide, the drugs of protozoan origin, were studied in comparison with known modifiers of biological reactions (bacterial polysaccharides, synthetic polypeptides) in combined radiation-thermal injuries (CRTI). The efficiency of drugs under examination was found to be time- and dose-dependent. Application of the drugs of protozoan nature 24 hours prior to or 1 hour following CRTI provided 30 to 50% survival of experimental animals for 30 days as compared to 4.

[The suppression of the chemiluminescence of mammalian cells by the metabolites of Crithidia oncopelti].

Effect of water-soluble (polypeptides) fraction (PF) of Crithidia oncopelti on the chemiluminescence of human blood cells and murine splenocytes was studied. The study has shown that PF possessed a capacity to inhibit oxygen-dependent killer system of polymorphonuclear leucocytes in dose-dependent manner. This effect was of a complex nature.

[New biologically active substances from protozoa and an evaluation of their action].

Novel biologically active substances were obtained from nonpathogenic for humans and free-living protozoa: Crithidia oncopelti, Trypanosoma lewisi, and Astasia longa. There were studied conditions of biosynthesis, composition and biological activity of the total lipid fraction from the protozoa species, sucrose ethers and fatty acids, the latter were isolated from A. longa--(astazilide preparation), reserve beta-1,3 glucan from A.

[The trypanocidal action of fluorouracil on Crithidia oncopelti in the presence of lipid metabolic modifiers].

Effect of fluorouracil in combination with ascorbate and L-valin, modifiers of lipid metabolism was studied in cultured protozoa Crithidia oncopelti. The inhibitory effect of preparation in a concentration of 100 mcg/ml gradually decreased in the course of cultivation. Its readdition to a 96-hour culture did not increase its effect on protozoa cells.

[Lactoferrin and fibrinogen degradation products in saliva].

The authors suggest a simple and convenient procedure for measuring acute-phase proteins, lactoferrin, fibrinogen and its degradation products, in mixed human saliva by the agar immunodiffusion test with appropriate monospecific antisera. Examinations of 1205 salivary samples have revealed that salivary fibrinogen degradation products and lactoferrin detection rates do not depend on the subject's sex but are growing with age and intensity of the aggressive admixture effects in inhaled air; they are also related to the intensity of the inflammatory and proliferative processes in the oral cavity and bronchopulmonary system, the values varying from 4-16 percent in health to 68.8-71.

[The biological activity of complex esters of saccharose and higher fatty acids].

Sucrose/fatty acid ethers from Astasia longa culture have been studied. Biological activity of different ethers has been studied on calf kidney cells. The activity depends on the lipid component of the ethers.

[Composition and immunological activity of membrane-bound surface glycoprotein from Crithidia oncopelti].

A membrane-bound glycoprotein with a molecular weight of 10000-12000 was isolated from Crithidia oncopelti and purified. The glycoprotein contained peptide, carbohydrate and lipid fragments and phosphorus. The peptide fragment was represented by 10 amino acids.

[The membrane-bound surface glycoprotein of Crithidia oncopelti].

Membrane-associated surface glycoprotein from Crithidia oncopelti (MAG) has been isolated and studied. Its molecular weight constitutes 10-12 kDa. MAG contains 12% protein, 29% carbohydrates, 1.

[Effect of astasilid on the stimulation of peritoneal macrophages].

The effect of astasilid, a sucrose monoester and the effect of mainly unsaturated fatty acids from the lipid fraction of Astasia longa on immunocompetent cells--macrophages of the mouse peritoneal cavity were studied. It was shown that astasilid increased 7.5-8.

[Astasian: A new stimulator of peritoneal macrophages].

Immunological properties of astasin, a novel substance isolated from Astasia longa was studied. 3-4 days after intraperitoneal administration of astasin in a dose of 1000 micrograms per a mouse (50 mg/kg) in the form of suspension in physiological solution or gel in dimethylsulfoxide there was observed maximum increase in the number of the Fc-receptor cells of the macrophage plasmatic membranes and intensity of sheep red blood cell phagocytosis (3.6-4 and 3.

[Rubro-caudate projections in the cat].

A few neurons projecting to the caudate nucleus were found in the cat red nucleus by the horse-radish peroxidase (HRP) method. Rubro-caudate neurons were observed both in the magnocellular and parvocellular parts of the red nucleus. Organization of the reciprocal connections of red nucleus and striopallidal system is discussed.

[Trypanosome sensitivity to polyene antibiotics].

Swelling of the plasma membrane is one of the mechanisms of resistance to damages in pathogenic Protozoa. Polyenic antibiotics induce reconstruction of the cytoplasma membrane of unicellular eukaryotic organisms, i. e.

[Effect of lipid preparations isolated from protozoa on experimental leishmaniasis in golden hamsters].

The effect of lipid preparations Nos. 59 and 60 isolated from Protozoa on experimental leishmaniosis of golden hamsters was studied. Preparations Nos.