In vivo immunostimulatory effect of the amoebocyte lysate and plasma of Asian horseshoe crab, in a piscine model.

Antimicrobial proteins/peptides are becoming a new generation of immunostimulants for prevention and disease control in human and animals, including aquatic animals. As the haemolymph of horseshoe crabs ( contains broad ranges of bioactive compounds, we have explored the in vivo immunostimulating potential of amoebocyte lysate and plasma using a fish model. Indian major carp, yearlings were injected intraperitoneally with two doses of lysate and plasma at 50 and 100 µg protein per fish.

Current prospects and challenges in fish vaccine development in India with special reference to Aeromonas hydrophila vaccine.

Infectious diseases are adversely affecting aquaculture practices throughout world and Asian countries are no exception. Indian aquaculture practices are facing serious setback due to a variety of infectious agent's which are responsible for severe mortality and morbidity of all the cultured freshwater fish species leading to severe economic losses. The emergence of antibiotic resistant pattern, residual effect and environmental degradation due to indiscriminate use of antibiotics has necessitates the development of suitable alternate prophylaxis measures for better protection.

Immune responses to live and inactivated Nocardia seriolae and protective effect of recombinant interferon gamma (rIFN γ) against nocardiosis in ginbuna crucian carp, Carassius auratus langsdorfii.

Looking into the fact that substantial mortality and morbidity is associated with intracellular Gram +ve bacterium, Nocardia seriolae infection, an effective vaccine against this pathogen is necessary to control the significant losses in aquaculture practices. Therefore, an attempt was made to evaluate the effect of live (sub-lethal) and inactivated (antigenic form) N. seriolae on cellular and humoral immunity in ginbuna crucian carp, Carassius auratus langsdorfii as well as the therapeutic potency of recombinant interferon gamma (rIFN γ) against N.