Preparation of dental resin composites with anti-bacterial adhesion against Streptococcus mutans using fluorinated and silicon containing dimethacrylates.

Objective: The purpose of this study was to enhance the anti-bacterial adhesion effect against Streptococcus mutans (S. mutans) of fluorinated dimethacrylate (DF MA) based dental resin composites (DRCs) by using silicone dimethacrylate (SMA-MEO).

Method: The SMA-MEO was added into mixture of DFMA and tricyclo (5.

Performance of low shrinkage Bis-EFMA based bulk-fill dental resin composites.

Objectives: The purpose of this study was evaluating the performance of new Bis-EFMA based bulk-fill composites with common methacrylate based composites and commercial dental composites.

Methods: The Bis-EFMA monomer was synthesized and the novel Bis-EFMA based bulk-fill composites were prepared. The resin composite samples were co-cultured with human gingival epithelial cells and human dental pulp stem cells to test the biocompatibility.

Odontogenic and anti-inflammatory effects of magnesium-doped bioactive glass in vital pulp therapy.

This study aimed to investigate the effects of magnesium-doped bioactive glass (Mg-BG) on the mineralization, odontogenesis, and anti-inflammatory abilities of human dental pulp stem cells (hDPSCs). Mg-BG powders with different Mg concentrations were successfully synthesized via the sol-gel method and evaluated using x-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy. Apatite formation was observed on the surfaces of the materials after soaking in simulated body fluid.

A low-shrinkage-stress and anti-bacterial adherent dental resin composite: physicochemical properties and biocompatibility.

Polymerisation shrinkage and biofilm accumulation are the two main problems associated with dental resin composites (DRCs) that induce secondary caries, which can cause restoration failure. Polymerisation shrinkage can lead to microleakage gaps between the tooth and the DRCs, causing the aggregation of bacteria and development of secondary caries. Reducing the shrinkage stress (SS) and improving the resistance to bacterial adhesion have always been the focus of this field in modifying DRCs.

The mechanism of biomineralization: Progress in mineralization from intracellular generation to extracellular deposition.

Biomineralization is a highly regulated process that results in the deposition of minerals in a precise manner, ultimately producing skeletal and dental hard tissues. Recent studies have highlighted the crucial role played by intracellular processes in initiating biomineralization. These processes involve various organelles, such as the endoplasmic reticulum(ER), mitochondria, and lysosomes, in the formation, accumulation, maturation, and secretion of calcium phosphate (CaP) particles.

Low shrinkage bulk-filled dental resin composites with non-estrogenic dimethacrylate.

This study synthesized and characterized different proportions of nonestrogenic di(meth)acrylate 9,9-bis[4-((2-(2-methacryloyloxy)ethyl-carbamate)ethoxy)phenyl] fluorine (Bis-EFMA)-based resin composite systems to study their physical, chemical, optical and biological characteristics, and adhesive properties after bonding to a tooth. The estrogenic activity of raw materials was evaluated and compared with estrogen and commercial bisphenol A. After photopolymerization, all resin composite systems were prepared, and their properties were systematically investigated.

New Materials and Their Applications: Perspectives in Restorative Dentistry and Endodontics.

"New Materials and Their Application: Perspectives in Restorative Dentistry and Endodontics" is a new open Special Issue of Materials, which aims to publish original and review articles on novel scientific research that closely relates to new dental materials and their synthesis, function, characteristics, and applications [...

Properties of Bis-GMA free bulk-filled resin composite based on high refractive index monomer Bis-EFMA.

With the aim to prepare Bis-GMA-free bulk-filled dental resin composite (DRC), Bis-GMA-free resin matrix was prepared by mixing Bis-EFMA with TEGDMA at two mass ratios (Bis-EFMA/TEGDMA = 50 wt/50 wt and 60 wt/40 wt), and the bulk-filled resin composites were then obtained by mixing resin matrix with silanated glass fillers at a mass ratio of 30 wt/70 wt. Bis-GMA based resin composites were used as control. Refractive indexes of resin matrixes were measured.

Promotion Effect of Carboxymethyl Chitosan on Dental Caries via Intrafibrillar Mineralization of Collagen and Dentin Remineralization.

Objective: To observe ultrastructural changes during the process of carboxymethyl chitosan (CMC)-mediated intrafibrillar mineralization, we evaluated the biomimetic remineralization potential of CMC in type-I collagen fibrils and membranes, and further explored the bond strength as well as the bond interfacial integrity of the biomimetic remineralized artificial caries-affected dentin (ACAD).

Methods: A mineralized solution containing 200 μg/mL CMC was used to induce type-I collagen biomimetic remineralization in ACAD, while traditional mineralization without CMC was used as a control. The process and pattern of mineralization were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS) as well as structured illumination microscopy (SIM).

Preparation of Bis-GMA free dental resin composites with anti-adhesion effect against Streptococcus mutans using synthesized fluorine-containing methacrylate (DFMA).

With purpose of preparing Bis-GMA free dental resin composites (DRCs) with anti-adhesion effect against Streptococcus mutans (S. mutans), a new fluorinated dimethacrylate (DFMA) was synthesized and used as base resin of DRCs. Two reactive diluents TEGDMA and SR833s were mixed with DFMA separately to prepare resin matrixes.

Effect of poly (γ-glutamic acid)/tricalcium phosphate (γ-PGA/TCP) composite for dentin remineralization in vitro.

The poly (γ-glutamic acid)/tricalcium phosphate (γ-PGA/TCP) composite was fabricated as a novel biomineralization material function in preventing caries. Demineralized bovine dentin specimens were prepared and randomly divided into 5 groups (i. α-TCP, ⅱ.

Influence of molecular weight and concentration of carboxymethyl chitosan on biomimetic mineralization of collagen.

The objective of the present study was to systematically investigate the influence of molecular weight (MW) and concentration of carboxymethyl chitosan (CMC), which served as non-collagenous protein (NCP) surrogates, on biomimetic mineralization of type I collagen. Supersaturated CMC-stabilized amorphous calcium-phosphate (CMC-ACP) dispersions containing different MWs (20 kDa, 60 kDa, 150 kDa) and concentrations (25, 50, 100, 200, 400 μg ml) of CMC were prepared. After mineralization in the aforementioned dispersions for 7 days, the pattern and extent of biomimetic mineralization of collagen scaffolds were investigated.

A universal adhesive incorporating antimicrobial peptide nisin: effects on Streptococcus mutans and saliva-derived multispecies biofilms.

For purpose of enhancing the antibacterial activity of a universal adhesive, the antimicrobial peptide nisin was incorporated into Single Bond Universal and its antibacterial effect on Streptococcus mutans monospecific biofilms and saliva-derived multispecies biofilms was studied. Nisin was incorporated into Single Bond Universal and the antibacterial activity was examined by confocal laser scanning microscopy (CLSM), reverse transcription-quantitative polymerase chain reaction (qRT-PCR), phenol-sulfuric acid method and lactate dehydrogenase enzymatic method. The bonding properties were tested by microtensile bond strength (μTBS) and degree of conversion (DC).

The inhibitory effect of carboxyl-terminated polyamidoamine dendrimers on dentine host-derived matrix metalloproteinases in an etch-and-rinse adhesive system.

The biomimetic remineralization of collagen fibrils has increased interest in restoring the demineralized dentine generated by dental caries. Carboxyl-terminated polyamidoamine dendrimers (PAMAM-COOH), hyperbranched polymeric macromolecules, can act as non-collagenous proteins to induce biomimetic remineralization on a dentine organic matrix. However, remineralization is an extremely time-consuming process; before complete remineralization, demineralized dentine collagen fibrils are susceptible to degradation by host-derived matrix metalloproteinases (MMPs).

Sodium Fluoride under Dose Range of 2.4-24 μM, a Promising Osteoimmunomodulatory Agent for Vascularized Bone Formation.

Fluoride has essential effects on bone physiological activity and is widely used in bone biomaterials modification. However, this beneficial effect is highly related to the dose range and improper dosing can lead to pathological conditions such as fluorosis of bone. Therefore, this study first investigated the dose dependent effect of fluoride on bone regeneration.

LL-37 inhibits LPS-induced inflammation and stimulates the osteogenic differentiation of BMSCs via P2X7 receptor and MAPK signaling pathway.

Oral diseases, such as periapical periodontitis and periodontitis, are characterized by inflammation-induced bone loss. LL-37, a human antimicrobial peptide (AMP), has multiple biological functions and the potential to promote osteogenesis. Therefore, this study aimed to investigate the regulatory effects of LL-37 within normal and inflammatory microenvironments.

Use of experimental-resin-based materials doped with carboxymethyl chitosan and calcium phosphate microfillers to induce biomimetic remineralization of caries-affected dentin.

This study investigated carboxymethyl chitosan (CMC)-induced biomimetic mineralization of collagen fibrils, with the aim of synthesizing experimental resins doped with CMC and calcium phosphate microfillers to remineralize artificial caries-affected dentin (ACAD) and enhance resin-dentin bonding durability. A size exclusion test provided evidence for the rejection of CMC (M 150 kDa) by collagen fibrils. Transmission electron microscopy and selected area electron diffraction conducted on reconstituted two-dimensional collagen showed typical deposition of needle-like hydroxyapatite crystals within collagen fibrils through CMC-induced biomimetic mineralization.

Antibacterial effect and bond strength of a modified dental adhesive containing the peptide nisin.

This study attempted to incorporate the antibacterial peptide nisin into an etch-and-rinse dental adhesive to evaluate the antibacterial activity of the modified adhesive against Streptococcus mutans and the bond strength. Single Bond 2 was used as a negative control, and nisin was incorporated at 1%, 3%, and 5% (w/v). The antibacterial activity against S.

Extrafibrillar collagen demineralization-based chelate-and-rinse technique bridges the gap between wet and dry dentin bonding.

Unlabelled: Limitations associated with wet-bonding led to the recent development of a selective demineralization strategy in which dentin was etched with a reduced concentration of phosphoric acid to create exclusive extrafibrillar demineralization of the collagen matrix. However, the use of acidic conditioners removes calcium via diffusion of very small hydronium ions into the intrafibrillar collagen water compartments. This defeats the purpose of limiting the conditioner to the extrafibrillar space to create a collagen matrix containing only intrafibrillar minerals to prevent collapse of the collagen matrix.

Intrafibrillar mineralization of polyacrylic acid-bound collagen fibrils using a two-dimensional collagen model and Portland cement-based resins.

The biomimetic remineralization of apatite-depleted dentin is a potential method for enhancing the durability of resin-dentin bonding. To advance this strategy from its initial proof-of-concept design, we sought to investigate the characteristics of polyacrylic acid (PAA) adsorption to desorption from type I collagen and to test the mineralization ability of PAA-bound collagen. Portland cement and β-tricalcium phosphate (β-TCP) were homogenized with a hydrophilic resin blend to produce experimental resins.

Potential applications of antimicrobial peptides and their mimics in combating caries and pulpal infections.

Unlabelled: Antimicrobial peptides (AMPs) are short cationic host-defense molecules that provide the early stage of protection against invading microbes. They also have important modulatory roles and act as a bridge between innate and acquired immunity. The types and functions of oral AMPs were reviewed and experimental reports on the use of natural AMPs and their synthetic mimics in caries and pulpal infections were discussed.

[Biomodifying effect of epigallocatechin-3-gallate on dentine substrate splicing surface].

Objective: To investigate the effect of epigallocatechin-3-gallate (EGCG) on biomodification of demineralized dentine substrate, in its permeability, hydrophobicity, and inhibition ability to collagen enzymatic degradation.

Methods: The dentine substrates were treated with simulated pulpal pressure created by mixtures of 0.02%, 0.

Effects of fluoridation of porcine hydroxyapatite on osteoblastic activity of human MG63 cells.

Biological hydroxyapatite, derived from animal bones, is the most widely used bone substitute in orthopedic and dental treatments. Fluorine is the trace element involved in bone remodeling and has been confirmed to promote osteogenesis when administered at the appropriate dose. To take advantage of this knowledge, fluorinated porcine hydroxyapatite (FPHA) incorporating increasing levels of fluoride was derived from cancellous porcine bone through straightforward chemical and thermal treatments.

[Sealing properties of three resin-based sealers].

Objective: To evaluate the sealing properties of three resin- based sealers, EndoREZ, RealSEAL and RealSEAL SE.

Methods: Forthy-eight extracted human anterior teeth with single root and canal were prepared using ProTaper files with crown-down technique to F3. The teeth were filled with three sealer respectively with hot gutta- percha vertical condensation technique simulating the clinical situation.

Organic anion composition of human whole saliva as determined by ion chromatography.

Background: The majority of studies examining anionic composition in human saliva have focused on inorganic anions only, and accompanying organic anion concentration has often been overlooked in the development of artificial salivas. The purpose of this study was to examine the major organic anionic species profile of human saliva, with a view to gaining further insight into human salivary chemistry.

Methods: Unstimulated whole saliva was collected from 11 healthy volunteers over a period of 18months.