Corrigendum: Polymerase II-associated factor 1 complex-regulated -Clade genes repress flowering in response to chilling.

[This corrects the article DOI: 10.3389/fpls.2022.

Chloroplasts prevent precocious flowering through a GOLDEN2-LIKE-B-BOX DOMAIN PROTEIN module.

The timing of flowering is tightly controlled by signals that integrate environmental and endogenous cues. Sugars produced by carbon fixation in the chloroplast are a crucial endogenous cue for floral initiation. Chloroplasts also convey information directly to the nucleus through retrograde signaling to control plant growth and development.

FLOWERING LOCUS M isoforms differentially affect the subcellular localization and stability of SHORT VEGETATIVE PHASE to regulate temperature-responsive flowering in Arabidopsis.

Temperature is an important environmental cue that affects flowering time in plants. The MADS-box transcription factor FLOWERING LOCUS M (FLM) forms a heterodimeric complex with SHORT VEGETATIVE PHASE (SVP) and controls ambient temperature-responsive flowering in Arabidopsis. FLM-β and FLM-δ, two major splice variants produced from the FLM locus, exert opposite effects on flowering, but the molecular mechanism by which the interaction between FLM isoforms and SVP affects temperature-responsive flowering remains poorly understood.

Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions.

Protein-lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids participate in these interactions remains challenging. assays to assess the physical interaction between a protein of interest and a panel of phospholipids provide crucial information for predicting the functionality of these interactions . In this protocol, which we developed in the context of evaluating protein-lipid binding of the florigen FLOWERING LOCUS T, we describe four independent experiments to determine the interaction of a protein with phospholipids: lipid-protein overlay assays, liposome binding assays, biotin-phospholipid pull-down assays, and fluorescence polarization assays.

Polymerase II-Associated Factor 1 Complex-Regulated -Clade Genes Repress Flowering in Response to Chilling.

RNA polymerase II-associated factor 1 complex (PAF1C) regulates the transition from the vegetative to the reproductive phase primarily by modulating the expression of () and [, also known as ()] at standard growth temperatures. However, the role of PAF1C in the regulation of flowering time at chilling temperatures (i.e.

Florigen sequestration in cellular membranes modulates temperature-responsive flowering.

Plants respond to temperature changes by modulating florigen activity to optimize the timing of flowering. We show that the mobile florigen FLOWERING LOCUS T (FT) interacts with the negatively charged phospholipid phosphatidylglycerol (PG) at cellular membranes and binds the lipid bilayer. Perturbing PG biosynthesis in phloem companion cells leads to temperature-insensitive early flowering.

Nonsense-mediated mRNA decay modulates Arabidopsis flowering time via the SET DOMAIN GROUP 40-FLOWERING LOCUS C module.

The nonsense-mediated mRNA decay (NMD) surveillance system clears aberrant mRNAs from the cell, thus preventing the accumulation of truncated proteins. Although loss of the core NMD proteins UP-FRAMESHIFT1 (UPF1) and UPF3 leads to late flowering in Arabidopsis, the underlying mechanism remains elusive. Here, we showed that mutations in UPF1 and UPF3 cause temperature- and photoperiod-independent late flowering.

Regulation of flowering time by ambient temperature: repressing the repressors and activating the activators.

Plants display remarkable developmental flexibility as they continuously sense and respond to changes in their environment. This flexibility allows them to select the optimal timing for critical developmental decisions such as when to flower. Ambient temperature is a major environmental factor that influences flowering; the mechanisms involved in ambient temperature-responsive flowering have attracted particular attention as a consequence of the effects of global climate change on temperature.

Profiling Protein-DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis.

In plant cells, transcription factors play an important role in the regulation of gene expression, which eventually leads to the formation of complex phenotypes. Although chromatin immunoprecipitation (ChIP) involves a lengthy process that requires up to 4 days to complete, it is a powerful technique to investigate the interactions between transcription factors and their target sequences in vivo. Here, we describe a detailed ChIP protocol, focusing on ChIP-qPCR, from material collection to data analyses.

PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE 1 modulates flowering in a florigen-independent manner by regulating .

PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE 1 (PECT1) regulates phosphatidylethanolamine biosynthesis and controls the phosphatidylethanolamine:phosphatidylcholine ratio in Previous studies have suggested that PECT1 regulates flowering time by modulating the interaction between phosphatidylcholine and FLOWERING LOCUS T (FT), a florigen, in the shoot apical meristem (SAM). Here, we show that knockdown of by artificial microRNA in the SAM () accelerated flowering under inductive and even non-inductive conditions, in which transcription is almost absent, and in double mutants under both conditions. Transcriptome analyses suggested that PECT1 affects flowering by regulating () and ().

Role of , , and in Plant Immunity under Nonsense-Mediated mRNA Decay-Compromised Conditions at Low Temperatures.

Nonsense-mediated mRNA decay (NMD) removes aberrant transcripts to avoid the accumulation of truncated proteins. NMD regulates nucleotide-binding, leucine-rich repeat (NLR) genes to prevent autoimmunity; however, the function of a large number of NLRs still remains poorly understood. Here, we show that three NLR genes (, , and ) are important for NMD-mediated regulation of defense signaling at lower temperatures.

Evolution and functional diversification of FLOWERING LOCUS T/TERMINAL FLOWER 1 family genes in plants.

Plant growth and development, particularly the induction of flowering, are tightly controlled by key regulators in response to endogenous and environmental cues. The FLOWERING LOCUS T (FT)/TERMINAL FLOWER 1 (TFL1) family of phosphatidylethanolamine-binding protein (PEBP) genes is central to plant development, especially the regulation of flowering time and plant architecture. FT, the long-sought florigen, promotes flowering and TFL1 represses flowering.

Psychometric Functions of Vowel Detection and Identification in Long-Term Speech-Shaped Noise.

Purpose The goal of this study was to investigate vowel detection and identification in noise and provide baseline data regarding how vowel perception changed with signal-to-noise ratios. Psychometric functions of vowel detection and identification for 12 American English isolated vowels in long-term speech-shaped noise were examined for young listeners with normal hearing in this study. Method Vowel detection was measured at sensation levels from -10 to +5 dB (re: thresholds of vowel detection from the study of Liu and Eddins, 2008a ) with a 4-interval forced-choice procedure.

English Consonant Identification in Multi-Talker Babble: Effects of Chinese-Native Listeners' English Experience.

The identification of English consonants in quiet and multi-talker babble was examined for three groups of young adult listeners: Chinese in China, Chinese in the USA (CNU), and English-native listeners. As expected, native listeners outperformed non-native listeners. The two non-native groups had similar performance in quiet, whereas CNU listeners performed significantly better than Chinese in China listeners in babble.

TWIN SISTER OF FT (TSF) Interacts with FRUCTOKINASE6 and Inhibits Its Kinase Activity in Arabidopsis.

In flowering plants, the developmental switch to the reproductive phase is tightly regulated and involves the integration of internal and external signals. FLOWERING LOCUS T (FT) and TWIN SISTER OF FT (TSF) integrate signals from multiple pathways. FT and TSF function as florigenic substances, and share high sequence similarity with mammalian Raf kinase inhibitor protein (RKIP).

A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in mesophyll protoplasts.

Background: Binding of transcription factors to their target sequences is a primary step in the regulation of gene expression and largely determines gene regulatory networks. Chromatin immunoprecipitation (ChIP) is an indispensable tool used to investigate the binding of DNA-binding proteins (e.g.

Base-pair opening dynamics of the microRNA precursor pri-miR156a affect temperature-responsive flowering in Arabidopsis.

Internal and environmental cues, including ambient temperature changes, regulate the timing of flowering in plants. Arabidopsis miR156 represses flowering and plays an important role in the regulation of temperature-responsive flowering. However, the molecular basis of miR156 processing at lower temperatures remains largely unknown.

Structural determinants of miR156a precursor processing in temperature-responsive flowering in Arabidopsis.

MicroRNAs originate from primary transcripts (pri-miRNAs) containing hairpin structures. Plant pri-miRNAs have highly variable structures and little is known about the information encoded in their secondary structures. Arabidopsis miR156 is an ambient temperature-responsive miRNA and plays an important role in regulating flowering time.

Asphyxiation Incidents by Hydrogen Sulfide at Manure Storage Facilities of Swine Livestock Farms in Korea.

Livestock workers are involved in a variety of tasks, such as caring for animals, maintaining the breeding facilities, cleaning, and manure handling, and are exposed to health and safety risks. Hydrogen sulfide is considered the most toxic by-product of the manure handling process at livestock facilities. Except for several reports in developed countries, the statistics and cause of asphyxiation incidents in farms have not been collected and reported systematically, although the number of these incidents is expected to increase in developing and underdeveloped countries.

Auditory detection of non-speech and speech stimuli in noise: Effects of listeners' native language background.

This study investigated whether native listeners processed speech differently from non-native listeners in a speech detection task. Detection thresholds of Mandarin Chinese and Korean vowels and non-speech sounds in noise, frequency selectivity, and the nativeness of Mandarin Chinese and Korean vowels were measured for Mandarin Chinese- and Korean-native listeners. The two groups of listeners exhibited similar non-speech sound detection and frequency selectivity; however, the Korean listeners had better detection thresholds of Korean vowels than Chinese listeners, while the Chinese listeners performed no better at Chinese vowel detection than the Korean listeners.

FLOWERING LOCUS T has higher protein mobility than TWIN SISTER OF FT.

In plants, successful reproduction requires the proper timing of flowering under changing environmental conditions. Arabidopsis FLOWERING LOCUS T (FT), which encodes a proposed phloem-mobile florigen, has a close homologue, TWIN SISTER OF FT (TSF). During the vegetative phase, TSF shows high levels of expression in the hypocotyl before FT induction, but the tsf mutation does not have an apparent flowering-time phenotype on its own under long-day conditions.

English vowel identification in quiet and noise: effects of listeners' native language background.

Purpose: To investigate the effect of listener's native language (L1) and the types of noise on English vowel identification in noise.

Method: Identification of 12 English vowels was measured in quiet and in long-term speech-shaped noise and multi-talker babble (MTB) noise for English- (EN), Chinese- (CN) and Korean-native (KN) listeners at various signal-to-noise ratios (SNRs).

Results: Compared to non-native listeners, EN listeners performed significantly better in quiet and in noise.