Two in One Gram Negative Antibacterial Agent and Organic Dye Photocatalyst from Green Synthesized Ocimum Sanctum-Based N and O Co-Doped Carbon Dot Silver Nanocomposite.

This study reports, successful synthesis of Oxygen(O) and Nitrogen(N) co-doped Ocimum Sanctum plant-based or tulsi carbon dots-silver nanoparticle nanocomposites (TCD-AgNP) for the development of an efficient, highly active, low-cost fingerprint antibacterial agent against gram-negative organisms and a highly efficient photocatalyst for the degradation of methylene blue (MB). Green synthesized, high quantum yield (47 %), intensely blue fluorescent, highly stable N and O co-doped TCDs from carbonization technique of tulsi leaves is achieved without any chemical treatment or surface fascination which could act as an efficient green reducing agent for the development green TCD-AgNP nanocomposites. The novelty and advantage of this study is the development of highly stable, blue fluorescent, high quantum yield (40 %) environmental -friendly TCD-AgNP nanocomposite through reduction method by using green TCDs.

Improved production of alkaline and solvent-stable proteases from a halotolerant Exiguobacterium isolate through heterologous expression.

Halophiles are excellent sources of detergent proteases that are attributed to stability in alkaline pH, salts, surfactants, and hydrophobic solvents. The lower enzymatic yields and tedious downstream processes necessitate the search for newer halophilic sources. We have previously reported a halotolerant Exiguobacterium sp.

Pan-genomic comparison of a potential solvent-tolerant alkaline protease-producing sp. TBG-PICH-001 isolated from a marine habitat.

Unlabelled: The identification and applicability of bacteria are inconclusive until comprehended with genomic repositories. Our isolate, sp. TBG-PICH-001 exhibited excellent halo- and organic solvent tolerance with simultaneous production of alkaline protease/s (0.

Whole genome sequence data of TBG-201, a chitinolytic actinomycete isolated from the Vandanam sacred groves of Alleppey District, Kerala, India.

This study presents the complete genome sequence of TBG-201 isolated from the soil samples of Vandanam sacred groves in Alleppey District, Kerala, India. The organism has potent chitinolytic activity. The genome of TBG-201 was sequenced using the Illumina HiSeq-2500 platform with 2 × 150bp pair-end protocol and assembled using Velvet version 1.

The Anti-Biofilm Potential of Linalool, a Major Compound from , against and Its Toxicity Assessment in .

The anti-biofilm and anti-virulence potential of the essential oil (E.O.) extracted from M.

Genome Sequence Analysis of sp. Strain TBG-PICH-001, Isolated from Pichavaram Mangrove Forest in South India.

The draft genome sequence of sp. strain TBG-PICH-001, isolated from Pichavaram Mangrove Forest (Tamil Nadu, India), is reported here. Paired-end sequencing technology was used to sequence the genome on the Illumina HiSeq X Ten platform.

Evaluation of antibiofilm potential of four-domain α-amylase from Streptomyces griseus against exopolysaccharides (EPS) of bacterial pathogens using Danio rerio.

Biofilm formation is a major issue in healthcare settings as 75% of nosocomial infection arises due to biofilm residing bacteria. Exopolysaccharides (EPS), a key component of the biofilm matrix, contribute to the persistence of cells in a complex milieu and defends greatly from exogenous stress and demolition. It has been shown to be vital for biofilm scaffold and pathogenic features.

A highly divergent α-amylase from Streptomyces spp.: An evolutionary perspective.

The present study deals with the genetic changes observed in the protein sequence of an α-amylase from Streptomyces spp. and its structural homologs from Pseudoalteromonas haloplanktis, invertebrates and mammals. The structural homologs are renowned for their important features such as chloride binding triad and a serine-protease like catalytic triad (a triad which is reported to be strictly conserved in all chloride-dependent α-amylases).

Cloning, expression, homology modelling and molecular dynamics simulation of four domain-containing α-amylase from Streptomyces griseus.

In the present study, α-amylase from TBG19NRA1 was amplified, cloned and successfully expressed in BL21/DE3. Sequence analysis of α-amylase (SGAmy) revealed the presence of four domains (A, B, C and E). Alpha-amylases with E domain (also known as carbohydrate binding module 20 (CBM20)) are capable of degrading raw starch and this property holds great potential for application in starch processing industries.

Fukugiside, a biflavonoid from Garcinia travancorica inhibits biofilm formation of Streptococcus pyogenes and its associated virulence factors.

Purpose: Streptococcus pyogenes, a notorious human pathogen thatis responsible for various invasive and non-invasive diseases, possesses multiple virulence armaments, including biofilm formation. The current study demonstrates the anti-biofilm and anti-virulence potential of fukugiside, a biflavonoid isolated from Garciniatravancorica, against S. pyogenes.

A S52P mutation in the 'α-crystallin domain' of Mycobacterium leprae HSP18 reduces its oligomeric size and chaperone function.

Mycobacterium leprae HSP18 is a small heat shock protein (sHSP). It is a major immunodominant antigen of M. leprae pathogen.

Functional characterization of a small heat shock protein from Mycobacterium leprae.

Background: Small heat shock proteins are ubiquitous family of stress proteins, having a role in virulence and survival of the pathogen. M. leprae, the causative agent of leprosy is an uncultivable organism in defined media, hence the biology and function of proteins were examined by cloning M.

Chemical composition and antibacterial activity of the rhizome oil of Hedychium larsenii.

The composition of essential oil from the rhizomes of Hedychium larsenii M. Dan & Sathish was examined by GC-FID and GC-MS techniques. 99% of the oil consisted of monoterpenoids.