Genetic microevolution of clinical with reduced Amphotericin B sensitivity in China.

The global rate of Amphotericin B (AmB) resistance in has surpassed 12%. However, there is limited data on available clinical treatments and microevolutionary analyses concerning reduced AmB sensitivity. In this study, we collected 18 isolates from five patients between 2019 and 2022.

Evolutionary accumulation of 1 mutations from clinical echinocandin-resistant .

Introduction: Drug resistance to echinocandins, first-line drugs used to treat infection, is rapidly emerging. However, the accumulation of mutations in genes other than 1 (before an isolate develops to resistance via 1 mutations), remains poorly understood. Four clinical cases and 29 isolates associated with the incremental process of echinocandin resistance were collected and analyzed using antifungal drug susceptibility testing and genome sequencing to assess the evolution of echinocandin resistance.

The first rare case of infection reported in China and its genomic evolution in a human host environment.

Introduction: is a rare human pathogenic fungus, which has been poorly characterized at the genome level. In this study, we reported the first fatal case of infection in China and investigate the microevolution of in the human host environment.

Methods: A series of stains were collected from the patient at different time points for routine microbial and drug sensitivity testing.

Risk factors and cerebrospinal fluid indexes analysis of intracranial infection by Acinetobacter baumannii after neurosurgery.

Background: Intracranial infection by Acinetobacter baumannii () after neurosurgery has always been a difficult problem for neurosurgeons. This study analyzed risk factors that discriminated from other bacteria causing intracranial infection after neurosurgery. It also examined the differences in the cerebrospinal fluid (CSF) indexes to explore their value in the early diagnosis of intracranial infection by

Methods: We retrospectively reviewed ten years (January 2011 to May 2021) of postoperative central nervous system (CNS) infections in the First Hospital of China Medical University.

Phenotypic and genetic features of a novel clinically isolated rough morphotype .

Introduction: is a newly emerging pathogenic fungus of global concern and has been defined by the World Health Organization (WHO) as a member of the critical group of the most health-threatening fungi.

Methods: This study reveals and reports for the first time that a rough morphotype strain causes urinary tract infections in non-intensive care unit (ICU) inpatients. Furthermore, the morphology, the scanning electronmicroscopy (SEM), Whole-genome resequencing and RNA sequencing of possessing rough morphotype colonies compared to their smooth morphotype counterparts.

Carbapenem-resistant Enterobacter cloacae complex in a tertiary Hospital in Northeast China, 2010-2019.

Background: Carbapenem-resistant Enterobacter cloacae complex (CREC) is a new emerging threat to global public health. The objective of the study was to investigate the clinical characteristics and molecular epidemiology of CREC infections in the medical center of northeast China.

Methods: Twenty-nine patients were infected/colonized with CREC during a ten-year period (2010-2019) by WHONET analysis.

Genomic epidemiology of in a general hospital in Shenyang, China: a three-year surveillance study.

is an emerging pathogenic fungal species found worldwide. Since April 2016, colonization/infection cases have been found in a general hospital in Shenyang, China. The genome-based phylogenetic studies of these isolates remain undefined.

Is the superbug fungus really so scary? A systematic review and meta-analysis of global epidemiology and mortality of Candida auris.

Background: Candida auris is a new pathogen called "superbug fungus" which caused panic worldwide. There are no large-scale epidemiology studies by now, therefore a systematic review and meta-analysis was undertaken to determine the epidemic situation, drug resistance patterns and mortality of C. auris.

Clinical Characteristics and Outcomes of Candidemia Caused by Meyerozyma guilliermondii Complex in Cancer Patients Undergoing Surgery.

Although Meyerozyma guilliermondii complex is an uncommon cause of invasive candidiasis worldwide, reported cases, mainly regarding bloodstream infections, increased over years, and patients with cancer who have undergone recent surgery are most commonly affected. However, the clinical characteristics and outcomes of candidemia caused by M. guilliermondii complex remain poorly understood.

Antimicrobial resistance, genotypic characterization and pulsed-field gel electrophoresis typing of extended spectrum β-lactamases-producing clinical Escherichia coli strains in Macao, China.

Background: The rise of the production of CTX-M class extended spectrum β-lactamases (ESBLs) has been well documented in traveling countries but no data are found for Macao, an international travel city. The objectives of this study were to identify the antimicrobial resistance pattern, and determine the prevalence, genotype and clonal relationship of ESBLs in 209 clinical Escherichia coli strains from Macao, China.

Methods: Antimicrobial susceptibility test was performed to determine the resistance patterns of the isolates using the disk diffusion method with 17 antimicrobial agents.

[Molecular epidemiological study of multi-drug resistant Acinetobacter baumannii].

Objective: To determine the possible genetic background and the source of our hospital's 43 clinical isolates of multidrug-resistant Acinetobacter baumannii, and the category of gene cassettes in type 1 integrons of all strains.

Methods: Restriction enzyme Apa I was chosen for all strains in pulsed-field gel electrophoresis (PFGE) methods. Multilocus sequence typing (MLST) was used to compare the allelic profiles of all the strains.

[Novel gene sequence variants of pbp2b in penicillin-nonsusceptible Streptococcus pneumonia isolates].

Objective: To investigate the alternations in gene/amino acid sequence of penicillin-binding protein (PBP)2b from clinical isolates of penicillin-nonsusceptible Streptococcus pneumonia (PNSP) in this region.

Methods: 24 strains of Streptococcus pneumonia were collected from January to December 2006. The antibiotics susceptibility of these strains was detected.