High-mobility group box 1 as a surrogate prognostic marker in dogs with systemic inflammatory response syndrome.

Objective: To evaluate various surrogate markers associated with the inflammatory and counter-inflammatory responses with respect to mortality in dogs with systemic inflammatory response syndrome (SIRS).

Design: Prospective observational study.

Setting: Veterinary Teaching Hospital.

Apoptosis-like change, ROS, and DNA status in cryopreserved canine sperm recovered by glass wool filtration and Percoll gradient centrifugation techniques.

The ability of glass wool filtration (GWF) and Percoll density gradient centrifugation (PDGC) to remove spermatozoa with phosphatidylserine (PS) translocation of membrane, high levels of ROS, and DNA fragmentation was assessed by flow cytometry in frozen-thawed canine sperm. Ejaculates from 5 dogs were cryopreserved and thawed. Thawed sperm was processed by GWF and PDGC.

Effects of cryopreservation on phosphatidylserine translocation, intracellular hydrogen peroxide, and DNA integrity in canine sperm.

Evaluating cryoinjury of canine spermatozoa is crucial to improving the probability of fertilization. Recently, studies on sperm ROS production, phospholipid scrambling, and DNA damage induced by cryopreservation have been reported. However, the consequences of cryopreservation on these crucial factors are lacking with respect to canine semen.

A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen.

The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen. Sephadex, glass wool and Percoll gradient separation techniques were used for sperm separation and sperm motility, morphology and membrane integrity were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique.

The suppression of zfpm-1 accelerates the erythropoietic differentiation of human CD34+ cells.

Erythropoiesis is a complex multistage process for the differentiation of mature erythrocytes from hematopoietic stem cells. The function of several transcription factors has been reported in hematopoietic stem cell differentiation. However, the molecular basis governing its functional behavior is unclear.

Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice.

Peroxiredoxins (Prxs) are a family of antioxidant proteins that reduce peroxide levels by using reducing agents such as thioredoxin. These proteins were characterized to have a number of cellular functions, including cell proliferation and differentiation and protection of specific proteins from oxidative damage. However, the physiological roles of the peroxiredoxins have not been determined.