Defining spatial nonuniformities of all ipRGC types using an improved Opn4 recombinase mouse line.

Intrinsically photosensitive retinal ganglion cells (ipRGCs) play a crucial role in several physiological light responses. In this study, we generate an improved Opn4 knockin allele (Opn4), which faithfully reproduces endogenous Opn4 expression and improves compatibility with widely used reporters. We evaluated the efficacy and sensitivity of Opn4 for labeling in retina and brain and provide an in-depth comparison with the extensively utilized Opn4 line.

A new recombinase mouse line to target intrinsically photosensitive retinal ganglion cells (ipRGCs).

Intrinsically photosensitive retinal ganglion cells (ipRGCs) play a crucial role in several physiological light responses. In this study we generate a new knock-in allele (), in which cre is placed immediately downstream of the start codon. This approach aims to faithfully reproduce endogenous expression and improve compatibility with widely used reporters.