Lectin-based estimation of glycated hemoglobin in diabetes mellitus.

This study was undertaken to distinguish between normal and diabetic subjects by lectin-glycated hemoglobin interaction. The quantitative precipitin method was performed for the interaction between glucose-specific lectin Concanavalin A (Con A) and the glucose-containing RBC-lysate for the estimation of calculated HbA1c% from a standard curve. The standard curve was prepared by plotting the optical density of the precipitin for the interaction of standard HbA1c concentration with Con A against HbA1c reference standard.