Arbuscular Mycorrhizal Fungi Ameliorate Selenium Stress and Increase Antioxidant Potential of Zea mays in Seleniferous Soil.

The indigenous arbuscular mycorrhizal fungi (AMF) spores were isolated from rhizosphere soil associated with maize plants grown in natural selenium-impacted agricultural soils present in north-eastern region of Punjab, India (32°46' N, 74°46' N), with selenium concentration ranging from 2.1 to 6.1 mg kg dry weight, and their role in plant growth promotion, mitigation of selenium stress and phytochemical and antioxidant potential of host maize plants in natural seleniferous soil were examined.

Two-dimensional Graphene/MoS2 vertical heterostructure for detection of hemoglobin concentration in blood samples.

This study demonstrates the use of computational methods to simulate the molecular dynamics involved in hemoglobin concentration sensing, utilizing Material Studio and the TCAD Silvaco device simulator. A non-invasive and flexible Graphene/MoS2 heterostructure has been proposed for sensing hemoglobin concentration in blood samples. The findings reveal a notable shift in the wavelength-dependent refractive index and extinction coefficient, as well as significant changes in the absorption coefficient and reflectivity of the Graphene/MoS2 heterostructure in response to different hemoglobin concentrations, specifically within an approximate range of 0.

Genome Sequence Analysis of Calcifying Bacteria Bacillus paranthracis CT5 and Its Biomineralization Efficacy to Improve the Strength and Durability Properties of Civil Structures.

Bacteria producing urea amidohydrolases (UA) and carbonic anhydrases (CA) are of great importance in civil engineering as these enzymes are responsible for microbially induced calcium carbonate precipitation (MICCP). In this investigation, genomic insights of Bacillus paranthracis CT5 and the expression of genes underlying in MICCP were studied. B.

Diversity of arbuscular mycorrhizal fungi in seleniferous soils and their role in plant growth promotion.

The present study aimed to investigate the molecular diversity of arbuscular mycorrhizal fungi (AMF) in natural seleniferous soils and their role in protecting plants from Se toxicity. The genomic DNA extracted from maize roots grown in seleniferous and non-seleniferous regions was amplified using AMF-specific primers by nested PCR. The 1.

Removal of cadmium and arsenic from water through biomineralization.

Due to anthropogenic activities, heavy metals such as cadmium (Cd) and arsenic (As) are one of the most toxic xenobiotics contaminating water, thus affecting human health and the environment. The objective of the present investigation was to study the effect of ureolytic bacteria Bacillus paramycoides-MSR1 for the bioremediation of Cd and As from contaminated water. The B.

Canine ocular thelaziosis: first report in Andhra Pradesh.

Thelaziosis is a vector-borne zoonotic disease with worldwide distribution, including in India and reports on canine ocular thelaziosis are very limited. The present case documentation will provide basic information to initiate the geographical epidemiological studies which will help to prevent the spread to these zoonotic diseases in the specified location. This communication reports about the rare case of canine ocular thelaziosis in Andhra Pradesh with the morphological features of the parasite and the management of ocular manifestations with suitable antimicrobial and anti-inflammatory eye drops along with ivermectin injections.

Graphene oxide reinforced chitosan/polyvinyl alcohol antibacterial coatings on stainless steel surfaces exhibit superior bioactivity without human cell cytotoxicity.

The study proposes an alternative therapeutics to diminish bacterial attachment in biomedical implants by modifying their surface with passive coatings. A uniform, thin-film of chitosan/polyvinyl alcohol/graphene oxide (CS/PVA/GO) was coated on 316 L stainless steel (SS) surface through spread casting followed by solvent evaporation. The abundant anchoring sites available at macromolecular interfaces of chitosan/PVA matrix facilitated a smooth, dense loading of GO.

Risk factors, clinical indicators, and pathological findings of abomasal ulcers in tropical dairy buffaloes.

The current study aimed at identifying the risk factors and initial diagnostic aids for abomasal ulcers. The risk factor analysis confirmed a significant association (P < 0.05) of abomasal ulcers with concentrate-rich diets (OR, 4.

Marginal Bone Loss Evaluation Postdental Implants Placed with Platelet-Rich Plasma: An Original Research.

Introduction: Various materials are used to improve the longevity of the dental implants. In our study, we assessed the marginal bone loss around dental implants after implantation with platelet-rich plasma.

Materials And Methods: We conducted a prospective clinical study among 200 subjects who were grouped equally as those with and without the application of PRF in the implantation.

Isolation and characterization of thermostable and alkali-tolerant cellulase from litter endophytic fungus Bartalinia pondoensis.

Endophytic fungi in plant tissues produce a wide range of secondary metabolites and enzymes, which exhibit a variety of biological activities. In the present study, litter endophytic fungi were isolated from a fire-prone forest and screened for thermostable cellulases. Among nine endophytic fungi tested, two isolates, Bartalinia pondoensis and Phoma sp.

evaluation of bioactive properties of banana sap.

Banana sap is currently designated as a waste subsequent to utilization of pseudo stem in pulp and paper industry as well as other applications which is contributing to the environmental pollution. In the present study, banana sap and its crude extracts were evaluated for antimicrobial, antioxidant and anticancer properties. The role of oxidized and un-oxidized banana sap for its antimicrobial potential against a microbial test panel comprising gram positive as well as gram negative bacteria and using micro broth dilution assay.

Abomasal impaction in buffaloes: Risk factors, clinical indicators, necropsy findings, and histology studies.

The present study aims to identify potential risk factors and most possible clinical indicators of abomasal impaction in buffaloes. Among the screened buffaloes (n = 636), the logistic model was fitted for 491 buffaloes because of the non-availability of parity-related data for 145 buffaloes. Third-trimester pregnancy, feeding paddy straw alone, and concurrent disorders were identified as the potential risk factors using multivariate regression analysis.

Biomineralization of cyanobacteria Synechocystis pevalekii improves the durability properties of cement mortar.

Microbially induced calcium carbonate precipitation (MICCP) is considered a novel eco-friendly technique to enhance the structural properties of cementitious-based material. Maximum studies have emphasized using ureolytic bacteria to improve the durability properties of building structures. In this study, the role of photoautotrophic bacteria Synechocystis pevalekii BDHKU 35101 has been investigated for calcium carbonate precipitation in sand consolidation, and enhancing mechanical and permeability properties of cement mortar.

Endophytic fungi: a potential source of industrial enzyme producers.

Microbial enzymes have gained interest for their widespread use in various industries and medicine due to their stability, ease of production, and optimization. Endophytic fungi in plant tissues produce a wide range of secondary metabolites and enzymes, which exhibit a variety of biological activities. The present review illustrates promising applications of enzymes produced by endophytic fungi and discusses the characteristic features of the enzymes, application of the endophytic fungal enzymes in therapeutics, agriculture, food, and biofuel industries.

Clinical, haemato-biochemical, and ultrasonographic findings of abomasal impaction and abomasal ulcers in buffaloes.

The current study evaluated the clinical, ruminal, haemato-biochemical, and ultrasonographic findings of abomasal disorders' affected buffaloes grouped as abomasal impaction (n = 18) and abomasal ulcers (n = 15). The abomasal disorders' screening was based on clinical examination and confirmed by ultrasonography. The most common clinical findings of the buffaloes affected with abomasal disorders include depressed demeanour (96.

Computational prediction of the effects of non-synonymous single nucleotide polymorphisms on the GPI-anchor transamidase subunit GPI8p of Plasmodium falciparum.

Drug resistance is increasingly evolving in malaria parasites; hence, it is important to discover and establish alternative drug targets. In this context, GPI-anchor transamidase (GPI-T) is a potential drug target primarily of its crucial role in the development and survival of the parasite in the GPI anchor biosynthesis pathway. The present investigation was undertaken to explore the plausible effects of nsSNP on the structure and functions of GPI-T subunit GPI8p of Plasmodium falciparum.

Screening of potent drug inhibitors against SARS-CoV-2 RNA polymerase: an in silico approach.

COVID-19 has emerged as a rapidly escalating serious global health issue, affecting every section of population in a detrimental way. Present situation invigorated researchers to look for potent targets, development as well as repurposing of conventional therapeutic drugs. NSP12, a RNA polymerase, is key player in viral RNA replication and, hence, viral multiplication.

Multi-metal tolerance of DHHC palmitoyl transferase-like protein isolated from metal contaminated soil.

The microbiota inhabiting in metal polluted environment develops strong defense mechanisms to combat pollution and sustain life. Investigating the functional genes of the eukaryotic microbiota inhabiting in these environments by using metatranscriptomics approach was the focus of this study. Size fractionated eukaryotic cDNA libraries (library A, < 0.

A robust genetic transformation protocol to obtain transgenic shoots of L. cultivar 'Kufri Chipsona 1'.

The genetic transformation of plants is an important biotechnological tool used for crop improvement for many decades. The present study was focussed to investigate various factors affecting genetic transformation of potato cultivar 'Kufri Chipsona 1'. It was observed that explants pre-cultured for 2 days on MS2 medium (MS medium containing 10 µM silver nitrate, 10 µM BA, 15 µM GA), injured with a surgical blade and co-cultivated with strain EHA105 [O.

Metatranscriptomics: an approach for retrieving novel eukaryotic genes from polluted and related environments.

Metatranscriptomics, a subset of metagenomics, provides valuable information about the whole gene expression profiling of complex microbial communities of an ecosystem. Metagenomic studies mainly focus on the genomic content and identification of microbes present within a community, while metatranscriptomics provides the diversity of the active genes within such community, their expression profile and how these levels change due to change in environmental conditions. Metatranscriptomics has been applied to different types of environments, from the study of human microbiomes, to those found in plants, animals, within soils and in aquatic systems.

Investigation of hub genes and their nonsynonymous single nucleotide polymorphism analysis in for designing therapeutic methodologies using next-generation sequencing approach.

Background: Incidences of resistance to current drugs by is increasing, hence, it is necessary to investigate and explore new drug targets to combat malarial disease.

Objective: Analysis of the transcriptome sequence information to characterize hub genes and their nonsynonymous single nucleotide polymorphisms (nsSNPs) to derive therapeutic objectives for .

Materials And Methods: Differentially expressed genes between Ring and other stages of were identified using Cufflinks tool.

Arsenic toxicity and its mitigation in ectomycorrhizal fungus Hebeloma cylindrosporum through glutathione biosynthesis.

Arsenic (As) contamination is one of the most daunting environmental problem bothering the whole world. Exploring a suitable bioremediation technique is an urgent need of the hour. The present study focusses on scrutinizing the ectomycorrhizal (ECM) fungus for its potential role in As detoxification and understanding the molecular mechanisms responsible for its tolerance.

Rare report of orchitis in a bull due to infection.

A 9 years old bull was presented to the hospital with a history of scrotal swelling for a period of 12 days and progressive emaciation with intermittent fever. Clinical examination revealed the swollen and painful scrotum with fluid thrill, mild abduction and disorientation of hindlimbs were noticed. Ultrasonography of the testes revealed the anechoic fluid in between the visceral and parietal layers of tunica vaginalis of testis with variation in the echogenicity of the testes.

Computational screening of potential drug targets for pathogens causing bacterial pneumonia.

Streptococcus pneumoniae is widely recognized as the main cause of bacterial pneumonia among all age groups. Other important gram-positive, gram-negative and atypical bacteria causing pneumonia majorly infect children and infants. Despite abundant occurrence of bacterial pneumonia, there is no specific antibiotic therapy available.

Heavy metal hypertolerant eukaryotic aldehyde dehydrogenase isolated from metal contaminated soil by metatranscriptomics approach.

Constant addition of heavy metal pollutants in soil resulting from anthropogenic activities can prove detrimental to both macro and micro life forms inhabiting the ecosystem. The potential functional roles of eukaryotic microbes in such environment were explored in this study by metatranscriptomics approach. Sized eukaryotic cDNA libraries, library A (<0.