Vps74p controls Golgi size in an Arf1-dependent manner.

The oncogene GOLPH3 is implicated in Golgi size regulation, a function yet to be experimentally linked to its PI4P effector function or the Golgi cisternal maturation in general. Moreover, its yeast homolog, Vps74p is not yet implicated in Golgi size regulation. Our results indicate that VPS74 deletion increases the late Golgi cisternal size and the cisternal maturation frequencies, and destabilizes the Golgi PI4P gradient in budding yeast.