The characteristic of HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1 alleles in Zhejiang Han population.

The Zhejiang Han population, a subgroup of the Southern Han ethnic group, resides in Zhejiang Province, situated on the southeast coast of China. In this study, we conducted HLA genotyping for 813 voluntary umbilical cord blood donors from the Zhejiang Han population, targeting 11 HLA loci, namely HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1, using the next-generation sequencing method. Our analysis of the alleles and haplotypes revealed a high degree of polymorphism within these loci.

Determination for KIR genotype and allele copy number via real-time quantitative PCR method.

Killer cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) play crucial roles in regulating NK cell activity. Here, we report a real-time quantitative PCR (qPCR) to genotype all KIR genes and their copy numbers simultaneously. With 18 pairs of locus-specific primers, we identified KIR genes by Ct values and determined KIR copy number using the 2 method.

Identification of the novel KIR3DP1*00604 allele in a Chinese individual.

KIR3DP1*00604 differs from KIR3DP1*0060101 by one single nucleotide substitution G > C at position 252.

The novel allele, HLA-B*15:627 was identified by next-generation sequencing in a Chinese cord blood donor.

HLA-B*15:627 shows one nucleotide substitution G to A at position 601 compared with HLA-B*15:02:01:01.

Characterization of the novel HLA-C*01:225 allele in a Chinese individual.

HLA-C*01:225 has one nucleotide change compared with HLA-C*01:02:01:01 in codon 110 of exon 3.

Allelic variation of KIR and HLA tunes the cytolytic payload and determines functional hierarchy of NK cell repertoires.

The functionality of natural killer (NK) cells is tuned during education and is associated with remodeling of the lysosomal compartment. We hypothesized that genetic variation in killer cell immunoglobulin-like receptor (KIR) and HLA, which is known to influence the functional strength of NK cells, fine-tunes the payload of effector molecules stored in secretory lysosomes. To address this possibility, we performed a high-resolution analysis of KIR and HLA class I genes in 365 blood donors and linked genotypes to granzyme B loading and functional phenotypes.

Simultaneous genotyping for human platelet antigen systems and and loci by targeted next-generation sequencing.

In order to treat the alloimmunization platelet transfusion refractoriness (PTR), human leukocyte antigen (HLA)-type and/or human platelet antigen (HPA)-type matched platelets between donors and patients are usually used. Therefore, genotyping of and loci, as well as HPA systems, for donors and patients, is of great significance. However, there is a rare report of genotyping for and loci as well as HPA systems at the same time.

Description of two novel HLA alleles: HLA-C*01:02:73 and HLA-C*01:02:75.

Compared with HLA-C*01:02:01:01, the alleles HLA-C*01:02:73 and HLA-C*01:02:75 each show one single nucleotide substitution respectively.

The novel HLA-DRB1*14:222N allele was identified by next-generation sequencing.

HLA-DRB1*14:222N differs from HLA-DRB1*14:03:01 by one single nucleotide substitution at position 262.

The novel HLA-DRB1*15:01:42 allele was identified by next-generation sequencing.

HLA-DRB1*15:01:42 differs from HLA-DRB1*15:01:01:01 by one single nucleotide substitution at position 732 C>T.

Identification of the novel HLA-DRB1*11:271 allele by next-generation sequencing.

HLA-DRB1*11:271 differs from HLA-DRB1*11:01:01:01 by a single nucleotide substitution at position 610G > A.

[Study of the distribution of KIR3DL2 alleles among ethnic Han Chinese from Zhejiang].

Objective: To study the distribution of KIR3DL2 alleles among ethnic Han Chinese from Zhejiang.

Methods: Genomic DNA was extracted by using a magnetic bead method. The full sequence of the KIR3DL2 gene was amplified with four pairs by PCR primers.

The novel HLA-A*26:174 allele was identified in a Chinese individual.

HLA-A*26:174 shows a single nucleotide substitution at position 148 G>T when compared to HLA-A*26:01:01:01.

Identification of the novel HLA-B*40:125:03 allele in a Chinese bone marrow donor.

HLA-B*40:125:03 shows a single nucleotide substitution at position 378 C > T when compared with HLA-B*40:125:02.

Identification of the novel KIR3DL2*00711 allele by sequencing-based typing in a Chinese individual.

KIR3DL2*00711 differs from KIR3DL2*0070101 by a single nucleotide substitution at position 1344G>A.

The combinatorial diversity of KIR and HLA class I allotypes in Peninsular Malaysia.

Killer cell immunoglobulin-like receptors (KIRs) interact with polymorphic human leucocyte antigen (HLA) class I molecules, modulating natural killer (NK) cell functions and affecting both the susceptibility and outcome of immune-mediated diseases. The KIR locus is highly diverse in gene content, copy number and allelic polymorphism within individuals and across geographical populations. To analyse currently under-represented Asian and Pacific populations, we investigated the combinatorial diversity of KIR and HLA class I in 92 unrelated Malay and 75 Malaysian Chinese individuals from the Malay Peninsula.

Identification of the novel HLA-A*02:837 and -A*02:888 alleles by next-generation sequencing in two Chinese individuals.

Compared with HLA-A*02:06:01:01, HLA-A*02:837 and HLA-A*02:888 show one single nucleotide substitution respectively.

KIR Variation in Iranians Combines High Haplotype and Allotype Diversity With an Abundance of Functional Inhibitory Receptors.

Natural killer (NK) cells are innate lymphocytes that eliminate infected and transformed cells. They discriminate healthy from diseased tissue through killer cell Ig-like receptor (KIR) recognition of HLA class I ligands. Directly impacting NK cell function, polymorphism associates with infection control and multiple autoimmune and pregnancy syndromes.

Novel method for simultaneously detecting HPA and HLA antibodies using Luminex microbeads.

Background: Alloantibodies against human platelet antigens (HPAs) and human leukocyte antigen (HLA) are implicated in several immune-mediated platelet disorders. Detection of these antibodies is crucial in the diagnosis and management of these disorders. The aim of this study was to establish a novel method to simultaneously detect HPA-1, HPA-2, HPA-3, HPA-5 and HLA antibodies with Luminex microbeads technology.