Publications by authors named "Suchan Niroula"
Article Synopsis
- CFTR modulator drugs improve lung function and body mass index in cystic fibrosis patients, but inflammation remains a challenge, indicating a need to target the underlying causes in the lungs of patients with established disease.* -
- The study explores the presence of unique, potentially pathogenic stem cell variants in cystic fibrosis lungs, similar to those found in COPD, and aims to identify which of these variants contribute to ongoing inflammation.* -
- Research utilized advanced stem cell cloning technology on end-stage CF lungs to identify five predominant stem cell variants, three of which are pro-inflammatory, highlighting the complexity of stem cell involvement in cystic fibrosis lung inflammation.*
The plurality of clonogenic cells derived from human lung includes a spectrum of diverse p63+ stem cells responsible for the regeneration of normal epithelial tissue and disease-associated metaplastic lesions. Here, we report protocols for the cloning, expansion, and characterization of these stem cell variants, which in general assist in analyses of stem cell heterogeneity, genome editing, drug screening, and regenerative medicine. For complete details on the use and execution of this protocol, please refer to Kumar et al.
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- * This study utilized single-cell cloning technologies to analyze lung tissue from COPD patients and controls, revealing that COPD lungs have distinct progenitor cells that contribute to disease symptoms and inflammation.
- * The research suggests that while these variant progenitor cells exist in healthy lungs, their excessive proliferation may trigger the damaging processes seen in COPD, implicating them in both normal and pathological lung functions.
'Adult' or 'somatic' stem cells harbor an intrinsic ability to regenerate tissues. Heterogeneity of such stem cells along the gastrointestinal tract yields the known segmental specificity of this organ and may contribute to the pathology of certain enteric conditions. Here we detail technology for the generation of 'libraries' of clonogenic cells from 1-mm-diamter endoscopic biopsy samples from the human gastrointestinal tract.
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