Mickey mouse-shaped laminated paper-based analytical device in simultaneous total cholesterol and glucose determination in whole blood.

The microfluidic paper-based analytical device (μPAD) platform is gaining attention as a low-cost, portable, and disposable detection tool. However, the limitations of traditional fabrication methods include poor reproducibility and the use of hydrophobic reagents. In this study, an in-house computer-controlled X-Y knife plotter and pen plotter were used to fabricate μPADs, resulting in a simple, more rapid, reproducible process that consumes less volume of reagents.