Publications by authors named "Suburamaniam Ramachandran"
An improved syngeneic orthotopic murine model of human breast cancer progression.
Breast Cancer Res Treat
October 2014
Article Synopsis
- * Current breast cancer preclinical models lack consensus, but a new method of orthotopic percutaneous implantation (ODV) into a specific area (chest 2nd mammary fat pad) shows more reliable results and better mimics human cancer progression.
- * ODV leads to tumors with different gene expression profiles, highlighting its potential advantages over subcutaneous (SQ) models, which may help improve breast cancer research efficiency and drug development outcomes.
Background: TWO MOST COMMONLY USED ANIMAL MODELS FOR STUDYING BREAST CANCER LUNG METASTASIS ARE: lung metastasis after orthotopic implantation of cells into the mammary gland, and lung implantations produced after tail vein (TV) injection of cells. Tail vein injection can produce lung lesions faster, but little has been studied regarding the differences between these tumors, thus, we examined their morphology and gene expression profiles.
Methods: Syngeneic murine mammary adenocarcinoma, 4T1-luc2 cells, were implanted either subcutaneously (Sq), orthotopically (OS), or injected via TV in Balb/c mice.