Cryostorage of silver barb (Barbodes gonionotus) semen in dry shipper: Efficacy, risk of bacterial cross-contamination and effects of Aeromonas hydrophila on post-thaw sperm quality.

This study was conducted to investigate the efficacy of dry shipper for the cryostorage of silver barb (Barbodes gonionotus) sperm, the subsequent risk of bacterial cross-contamination, and the effects of Aeromonas hydrophila on post-thaw sperm. Semen was diluted with calcium-free Hank's balanced salt solution containing 10% MESO, frozen at -8 °C/min and stored for 14 d in a dry shipper. A significant decline (P < 0.

In vitro inoculation of Aeromonas hydrophila and Pseudomonas fluorescens in cryopreserved silver barb (Barbodes gonionotus) milt: Effect on fertilization capacity and transmission potential to embryos.

Purposive use of cryopreserved sperm contaminated with pathogenic agents has increased the risk of spreading of fish diseases. The objective of this study was to investigate the effects of A. hydrophila subsp.

Influence of Aeromonas hydrophila and Pseudomonas fluorescens on motility, viability and morphometry of cryostored silver barb (Barbodes gonionotus) sperm.

This objective of the study was to evaluate the effects of A. hydrophila subsp. hydrophila and P.

Effect of antibiotic supplementation on the quality of cryopreserved fish sperm of silver barb (Barbodes gonionotus): Sperm motility and viability, bacterial quality and fertilization.

Disease prevention is a key aspect in developing cryopreservation procedures for fish sperm and in improving the reproductive biotechnology for commercially important aquatic species. The purpose of this study was to evaluate the effects of an antibiotic supplementation (0.25% penicillin-streptomycin, PS and 0.

Semen collection methods affect the bacterial composition of post-thawed semen of silver barb (Barbodes gonionotus).

Biosafety issue associated with the risk of pathogenic contamination of cryopreserved semen is a common concern because of associated declines in sperm quality, storage period and disease transmission. This study was conducted to evaluate the effects of methods of semen collection on sperm quality and bacterial composition of post-thawed semen of silver barb (Barbodes gonionotus). Semen collection methods consisted of four treatments: (1) hand-stripping of abdomen without rinsing of urogenital area with water, (2) hand-stripping of abdomen after rinsing of urogenital area with water, (3) catheterization without rinsing of urogenital area with water and (4) catheterization after rinsing of urogenital area with water.

The effect of extenders, cryoprotectants and cryopreservation methods on common carp (Cyprinus carpio) sperm.

The objective of this study was to determine the effects of various extenders, cryoprotectants and cryopreservation methods on post-thaw sperm motility and duration of sperm motility in common carp (Cyprinus carpio). We focused on freezing of common carp sperm utilizing a practical and inexpensive protocol for aquaculture. Sperm were diluted 1:1 in one of six extenders (common carp sperm extenders; CCSE 1-CCSE 6) containing three types of cryoprotectants (dimethyl sulfoxide; DMSO, methanol; MET and propylene glycol; PG) at a final concentration of 10%, and frozen at a rate of 10°C/min from an initial temperature 25 to -40°C before storage in liquid nitrogen.

Human TRP14 gene homologue from amphioxus Branchiostoma belcheri: identification, evolution, expression and functional characterization.

Thioredoxin-related protein of 14 kDa, TRP14, has previously been identified only in humans. Here we report the identification and expression of an amphioxus TRP14 gene, named AmphiTRP14, the first such data in a non-mammalian organism. AmphiTRP14 consists of a 372-bp open reading frame coding for a 123-amino-acid protein with a calculated molecular weight of 14 kDa.