Coumarin-Based Noncytotoxicity Fluorescent Dye for Tracking Actin Protein in In-Vivo Imaging.

shares maximum homology with the human disease-causing genes and thus has been employed to evaluate the toxicity of numerous compounds. Further, its distinguishable developmental stages, easy rearing, and short lifespan make it a perfect model organism to study toxicological properties of any new compound. The current study evaluates the toxic effect of a coumarin-based organic fluorescent dye, 7-hydroxy-4-methyl-8-((4-(2-oxo-2-chromen-3-yl)thiazol-2-ylimino)methyl)-2-chromen-2-one (), using as a model organism by studying different behavioral, screening, and staining techniques using flies.

Synthesis of First Coumarin Fluorescent Dye for Actin Visualization.

Selective fluorescence imaging of actin protein hugely depends on the fluorescently labeled actin-binding domain (ABD). Thus, it is always a challenging task to image the actin protein ( or ) directly with an ABD-free system. To overcome the limitations of actin imaging without an ABD, we have designed a facile and cost-effective red fluorescent coumarin dye 7-hydroxy-4-methyl-8-(4-(2-oxo-2-chromen-3-yl)thiazol-2-ylimino)methyl-2-chromen-2-one () for actin binding.

Through bond energy transfer (TBET)-operated fluoride ion sensing spirolactam ring opening of a coumarin-fluorescein bichromophoric dyad.

The detection of fluoride ions in a competitive environment often poses several challenges. In this work, we have designed and synthesized a coumarin functionalized fluorescein dyad (R3) which represents an ideal through bond energy transfer (TBET) fluorophore with the coumarin unit as donor and fluorescein unit as acceptor. The bichromophoric dyad demonstrates the detection of fluoride ions in the parts per billion (ppb) concentration level (22.

Butterfly pea (Clitoria ternatea) extract as a green analytical tool for selective colorimetric detection of bisulphate (HSO) ion in aqueous medium.

The blue color of butterfly pea (Clitoria ternatea) was extracted by Milli-Q water and evaluated for selective detection of bisulphate (HSO) ions. The stability of the Clitoria ternatea extract was established by UV-visible and fluorescence techniques. The blue water extract from Clitoria ternatea selectively recognizes HSO ions over various anions via a distinct visual color change from blue to purple with a significant hypsochromic shift of 68 nm in the UV-visible absorption spectra.

Fluorescence-based ion sensing in lipid membranes: a simple method of sensing in aqueous medium with enhanced efficiency.

Detection of ions in chemical, biological and environmental samples has gathered tremendous momentum considering the beneficial as well as adverse effects of the ions. Generally, most of the ions are beneficial up to an optimum concentration, beyond which they are toxic to human health. However, most of the fluorescence-based ion sensors are only active in non-aqueous solution because of the low solubility of the sensor molecules in aqueous buffer medium.

Sequential displacement strategy for selective and highly sensitive detection of Zn, Hg and S ions: An approach toward a molecular keypad lock.

A thiocarbonohydrazone locked salicylidene based macrocycle ligand L has been synthesized and its ion sensing properties were examined by UV-visible and fluorescence spectroscopy. The macrocycle serves as a highly selective colorimetric sensor for Hg ions while it acts as an excellent fluorescent sensor for Zn ions by exhibiting a green fluorescence at 498 nm even in the presence of interfering ions. A detailed analysis of binding characteristics such as complex stoichiometry, association constant and detection limits of L toward Hg and Zn ions were evaluated by UV-visible and fluorescence experiments which revealed a stronger binding affinity and higher detection limit of L toward the mercury ions.

Chromo-luminescent selective detection of fluoride ions by a copper(ii) bis(terpyridine) complex solution via a displacement approach.

Herein, for the first time, we have reported a copper(ii) bis(terpyridine) complex solution for instant 'naked eye' chromo-luminescent selective detection of fluoride ions in an acetonitrile medium at micromolar concentration. The copper complex [Cu(ii) (L)2] (NO3)2 [where L = 4'-(4-N,N'-dimethylaminophenyl)-2,2':6',2''-terpyridine] was characterized by mass spectroscopy and the terpyridine ligand by 1H NMR spectroscopy. The complex solution selectively discriminates F- ions from other anions such as AcO-, Br-, Cl-, CN-, H2PO4-, HSO4-, and I- in acetonitrile media via exceptional optical changes.