Polyphenols-loaded electrospun nanofibers in bone tissue engineering and regeneration.

Bone is a complex structure with unique cellular and molecular process in its formation. Bone tissue regeneration is a well-organized and routine process at the cellular and molecular level in humans through the activation of biochemical pathways and protein expression. Though many forms of biomaterials have been applied for bone tissue regeneration, electrospun nanofibrous scaffolds have attracted more attention among researchers with their physicochemical properties such as tensile strength, porosity, and biocompatibility.

Bioactive Zinc(II) complex incorporated PCL/gelatin electrospun nanofiber enhanced bone tissue regeneration.

Bone tissue regeneration is augmented by biocompatible nanofiber scaffolds, that supports reliable and enhanced bone formation. Zinc is an essential mineral that is vital for routine skeletal growth and it emerges to be able to improve bone regeneration. Phytochemicals, particularly flavonoids have achieved prominent interest for their therapeutic ability, they have demonstrated promising effects on bone by encouraging osteoblastogenesis, which finally leads to bone formation.

Biocompatible Porous Scaffolds of Chitosan/Poly(EG--PG) Blends with Tailored Pore Size and Nontoxic to Stem Cells: Preparation by Controlled Evaporation from Aqueous Acetic Acid Solution.

The preparation of porous films (average size variation from 1 to 32 μm) of a 1:1 blend of chitosan with poly(EG--PG) by the controlled evaporation of water from a 2 wt % aqueous acetic acid solution is reported. Interestingly, the blend exhibited porosity that could be tailored from 1 to 32 μm with the temperature of preparation of the blend film. The powder X-ray diffraction, Fourier transform infrared, and differential scanning calorimetry analyses of the films suggested the formation of partially miscible blends.

Green synthesis of copper oxide nanoparticles using sinapic acid: an underpinning step towards antiangiogenic therapy for breast cancer.

Synthesis of copper oxide nanoparticles without any chemical reductant is always a challenging methodology for biological studies. In this study, sinapic acid, a phytochemical, is used for the synthesis of stable copper oxide nanoparticles. The as-synthesized nanoparticles were characterized thoroughly using UV-Visible, IR spectroscopy, Transmission Electron Microscopy (TEM) and X-ray photoelectron spectroscopy (XPS).

Biocompatible hydrogels of chitosan-alkali lignin for potential wound healing applications.

Biocompatible hydrogels were prepared by mixing aqueous-acidic solution of chitosan with alkali lignin, a major by-product of the paper producing industries, for the first time, by sustainable means. Electrostatic interactions between the phenoxide groups in lignin and the ammonium groups on the chitosan backbone were found to be responsible for the ionotropic cross-linking. These gels were non-toxic to Mesenchymal stem cells, in vitro, and to zebrafish up to 100 μg/ml, in vivo.

Synthesis and characterization of silibinin/phenanthroline/neocuproine copper(II) complexes for augmenting bone tissue regeneration: an in vitro analysis.

In the recent decades, flavonoid metal complexes have been widely investigated for their multifaceted role in enabling osteoblast differentiation and bone formation. Silibinin complexed with copper(II) ion has been synthesized along with two mixed ligand complexes, namely copper(II) silibinin-phenanthroline and neocuproine as co-ligands, and their positive role in promoting neovacularization and osteoblast differentiation was investigated. Silibinin mono complex [Cu(sil)(HO)] and [Cu(sil)(phen)] showed similar UV-visible absorption in the region of 315 and 222 nm, whereas Cu(silibinin)(neocuproine) [Cu(sil)(neo)] showed a blueshift in the 320 nm transition.

Synthesis and characterization of zinc-silibinin complexes: A potential bioactive compound with angiogenic, and antibacterial activity for bone tissue engineering.

Zinc silibinin complex [Zn(sil)(HO)] and mixed ligand zinc complexes such as Zn(silibinin)(phenanthroline) [Zn(sil)(phen)], and Zn(silibinin)(neocuproine) [Zn(sil)(neo)] have been synthesized and characterized. The UV-vis spectra of the Zn(II) complexes showed a considerable shift in the intra-ligand transition. From the IR spectra, it is clear that carbonyl group in the C-ring is involved in the metal chelation besides A/C-ring hydroxyl group.

Mixed-ligand copper(II) complex of quercetin regulate osteogenesis and angiogenesis.

Copper(II) complex of quercetin Cu+Q, mixed ligand complexes, quercetin-Cu(II)-phenanthroline [Cu+Q(PHt)] and quercetin-Cu(II)-neocuproine [Cu+Q(Neo)] have been synthesized and characterized. From the FT-IR spectroscopic studies, it was evident that C-ring of quercetin is involved in the metal chelation in all the three copper complexes. C-ring chelation was further proven by UV-Visible spectra and the presence of Cu(II) from EPR spectroscopic investigations.

Oxidative environment causes molecular remodeling in embryonic heart-a metabolomic and lipidomic fingerprinting analysis.

Environmental factors including pollution affect human health, and the unifying factor in determining toxicity and pathogenesis for a wide array of environmental factors is oxidative stress. Here, we created the oxidative environment with 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH) and consequent cardiac remodeling in chick embryos. The metabolite fingerprint of heart tissue was obtained from Fourier transform infrared (FTIR) spectroscopic analysis.

DNA condensation by copper(II) complexes and their anti-proliferative effect on cancerous and normal fibroblast cells.

In our search towards copper(II) based anticancer compounds, copper(II) complexes [Cu(bitpy)2](ClO4)21, [Cu(bitpy)(phen)](NO3)22 and [Cu(bitpy)(NO3)](NO3) 3 were synthesized and characterized. All the three complexes contain the tridentate ligand bitpy, which bears biologically relevant benzimidazolyl head group, as one of the ligands. Because of the presence of the planar benzimidazolyl group in the bitpy ligand, the complexes exhibited intercalative mode of binding with DNA.

Investigation of nuclease, proteolytic and antiproliferative effects of copper(II) complexes of thiophenylmethanamine derivatives.

Four coordinate copper(II) complexes 1, 2 and 3 of ligands based on thiophenemethylamine containing imidazole, benzimidazole and pyridine moiety have been synthesized and characterized. Complex 1 has also been crystallographically characterized. The three complexes bind to DNA non-intercalatively, though partial intercalation in the case of complex 2 cannot be ruled out.

Copper(II) complexes of terpyridine derivatives: a footstep towards development of antiproliferative agent for breast cancer.

Two copper(II) complexes with terpyridyl conjugates, [Cu(meotpy)(dmp)](NO(3))(2) (1) and [Cu(bitpy)(dmp)](NO(3))(2) (2) where meotpy, bitpy and dmp stand for methoxybenzyl terpyridine, benzimidazolyl terpyridine and dimethyl phenanthroline respectively have been synthesized and characterized. Complex 1 has also been characterized crystallographically. Both the complexes have been found to bind CT-DNA intercalatively.

Anomalous behavior of pentacoordinate copper complexes of dimethylphenanthroline and derivatives of terpyridine ligands: Studies on DNA binding, cleavage and apoptotic activity.

Copper(II) complexes with substituted terpyridine ligands, namely [Cu(itpy)(dmp)](NO3)2 (1) and [Cu(ptpy)(dmp)](NO3)2 (2) have been synthesized and characterized. The interaction of the complexes with CT-DNA has been explored using spectroscopic techniques and viscosity. Complexes 1 and 2 bind in the grooves of DNA, interestingly 1 in the minor and 2 in the major groove.